Mark Arentshorst
Lab- en Projectmanager
- Naam
- Ing. M. Arentshorst
- Telefoon
- +31 71 527 4745
- m.arentshorst@biology.leidenuniv.nl
Lab- en Projectmanager
- Wiskunde en Natuurwetenschappen
- Instituut Biologie Leiden
- IBL Microbial Sciences
- Arentshorst M., Kooloth Valappil P., Mózsik L., Regensburg-Tuïnk A.J.G., Seekles S.J., Tjallings G., Fraaije M.W., Visser J. & Ram A.F.J. (2023), A CRISPR/Cas9‐based multicopy integration system for protein production in Aspergillus niger, FEBS Journal 290(21): 5127-5140.
- Seekles S.J., Brule T. van den, Punt M., Dijksterhuis J., Arentshorst M., Ljadpanahsaravj M., Roseboom W., Meuken G., Ongenae V.M.A., Zwerus J., Ohm R.A., Kramer G.J., Wösten H.A.B., Winde J.H. de & Ram A.F.J. (2023), Compatible solutes determine the heat resistance of conidia, Fungal Biology and Biotechnology 10: 21.
- Tjallinks G., Boverio A., Maric I., Rozeboom H., Arentshorst M., Visser J., Ram A.F.J., Mattevi A. & Fraaije M.W. (2023), Structure elucidation and characterization of patulin synthase, insights into the formation of a fungal mycotoxin, FEBS Journal 290(21): 5114-5126.
- Forn Cuni G., Welvaarts L., Stel F.M., Hondel C.A.M.J.J. van den, Arentshorst M., Ram A.F.J. & Meijer A.H. (2023), Stimulating the autophagic-lysosomal axis enhances host defense against fungal infection in a zebrafish model of invasive Aspergillosis, Autophagy 1986(1): 324-337.
- Seekles S.J., Dam J. van, Arentshorst M. & Ram A.F.J. (2022), Natural variation and the role of Zn2Cys6 transcription factors SdrA, WarA and WarB in sorbic acid resistance of aspergillus niger, Microorganisms 10(2): 221.
- Escobar N., Keizer E.M., Neer J.F. van, Arentshorst M., Strijp J.A.G. van, Haas P.J.A., Ram A.F.J., Punt P.J., Wösten H.A.B. & Cock H. de (2022), LaeA-dependent production of small molecules of Aspergillus niger that compete with specific antibodies that bind to human immune receptors bioRxiv. [Working paper].
- Demirci E., Arentshorst M., Yilmaz B., Swinkels A., Reid I.D., Visser J., Tsang A. & Ram A.F.J. (2021), Genetic characterization of mutations related to conidiophore stalk length development in aspergillus niger laboratory strain N402, Frontiers in Genetics 12: 666684.
- Arentshorst M., Di Falco M., Moisan M.C., Reid I.D., Spaapen T.O.M., Dam J. van, Demirci E., Powlowski J., Punt P.J., Tsang A. & Ram A.F.J. (2021), Identification of a conserved transcriptional activator-repressor module controlling the expression of genes involved in tannic acid degradation and gallic acid utilization in aspergillus niger, Fungal Biology 2: 681631.
- Reijngoud J., Arentshorst M., Ruijmbeek C., Reid I., Alazi E., Punt P.J., Tsang A. & Ram A.F.J. (2021), Loss of function of the carbon catabolite repressor CreA leads to low but inducer-independent expression from the feruloyl esterase B promoter in Aspergillus niger, Biotechnology Letters 43(7): 1323-1336.
- Leeuwe T.M. van , Arentshorst M., Punt P.J. & Ram A.F.J. (2020), Interrogation of the cell wall integrity pathway in Aspergillus niger identifies a putative negative regulator of transcription involved in chitin deposition, Gene: X 5: 100028.
- Leeuwe T.M. van, Gerritsen A., Arentshorst M., Punt P.J. & Ram A.F.J. (2020), Rab GDP-dissociation inhibitor gdiA is an essential gene required for cell wall chitin deposition in Aspergillus niger, Fungal Genetics and Biology 136: 103319.
- Jørgensen T.R., Burggraaf A.M., Arentshorst M., Schutze T., Lamers G.E.M., Niu J., Kwon M.J., Park J., Frisvad J.C., Nielsen K.F., Meyer V., Hondel C.A.M.J.J. van den, Dyer P.S. & Ram A.F.J. (2020), Identification of SclB, a Zn(II)2Cys6 transcription factor involved in sclerotium formation in Aspergillus niger, Fungal Genetics and Biology 139: 103377.
- Leeuwe T.M. van, Wattjes J., Niehues A., Forn-Cuní G., Geoffrion N., Mélida H., Arentshorst M., Molina A.,Tsang A., Meijer A.H., Moerschbacher B.M., Punt P.J. & Ram A.F.J. (2020), A seven-membered cell wall related transglycosylase gene family in Aspergillus niger is relevant for cell wall integrity in cell wall mutants with reduced α-glucan or galactomannan, Cell Surface 6: 100039.
- Leeuwe T.M. van, Arentshorst M., Forn-Cuní G., Geoffrion N., Tsang A., Delvigne F., Meijer A.H., Ram A.F.J. & Punt P.J. (2020), Deletion of the Aspergillus niger pro-protein processing protease gene kexB results in a pH-dependent morphological transition during submerged cultivations and increases cell wall chitin content , Microorganisms 8(12): 1918.
- Reijngoud J., Deseke M., Halbesma E.T.M., Alazi E., Arentshorst M., Punt P.J. & Ram A.F.R. (2019), Mutations in AraR leading to constitutive of arabinolytic genes in Aspergillus niger under depressing conditions, Applied Microbiology and Biotechnology 103(10): 4125-4136.
- Arentshorst M., Lange D. de, Park J., Lagendijk E.L., Alazi E., Hondel C.A.M.J.J. van den & Ram A.F.J. (2019), Functional analysis of three putative galactofuranosyltransferases with redundant functions in galactofuranosylation in Aspergillus niger, Archives of Microbiology 202(1): 197-203.
- Reijngoud J., Deseke M., Halbesma E.T.M., Alazi E., Arentshorst M., Punt P.J. & Ram A.F.J. (2019), Correction to: Mutations in AraR leading to constitutive of arabinolytic genes in Aspergillus niger under derepressing conditions, Applied Microbiology and Biotechnology 103(12): 5063-5063.
- Leeuwe T.M. van, Gerritsen A., Arentshorst M., Punt P.J. & Ram A.F.J. (2019), Rab GDP-dissociation inhibitor gdiA is an essential gene required for cell wall chitin deposition in aspergillus niger, Fungal Genetics and Biology 136: 103319.
- Leeuwe T.M. van, Arentshorst M., Ernst T., Alazi E., Punt P.J. & Ram A.F.J. (2019), Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi, Fungal Biology and Biotechnology 6: 13.
- Aerts D., Bergh S.G. van den, Post H., Altelaar M.A.F., Arentshorst M., Ram A.F.J., Ohm R.A. & Wösten H.A.B. (2019), FlbA-regulated gene rpnR is involved in stress resistance and impacts protein secretion when Aspergillus niger is grown on xylose, Applied and Environmental Microbiology 85(2): e02282-18.
- Aerts D., Hauer E.E., Ohm R.A., Arentshorst M., Teertstra W.R., Phippen C., Ram A.F.J., Frisvad J.C. & Wösten H.A.B. (2018), The FlbA-regulated predicted transcription factor Fum21 of Aspergillus niger is involved in fumonisin production, Antonie van Leeuwenhoek 111(3): 311-322.
- Alazi E., Knetsch T.G.J., Di Falco M., Reid I.D., Arentshorst M., Visser J., Tsang A. & Ram A.F.J. (2018), Inducer-independent production of pectinases in Aspergillus niger by overexpression of the D-galacturonic acid-responsive transcription factor gaaR, Applied Microbiology and Biotechnology 102(6): 2723–2736.
- Alazi E., Niu J., Otto S.B., Arentshorst M., Pham T.T.M., Tsang A. & Ram A.F.J. (2018), W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger, MicrobiologyOpen 8(5): e00732.
- Arentshorst M. & Ram A.F.J. (2018), Parasexual crossings for bulk segregant analysis in aspergillus niger to facilitate mutant identification via whole genome sequencing. In: Vries R. de, Tsang A. & Grigoriev I. (red.), Fungal Genomics. Methods in Molecular Biology nr. MMB 1775. New York, NY, U.S.A.: Humana Press. 277-287.
- Niu J., Alazi E., Reid I.D., Arentshorst M., Punt P.J., Visser J., Tsang A. & Ram A.F.J. (2017), An Evolutionarily Conserved Transcriptional Activator-Repressor Module Controls Expression of Genes for D-Galacturonic Acid Utilization in Aspergillus niger, Genetics 205(1): 169-183.
- Wanka F., Arentshorst M., Cairns T.C., Jorgensen T., Ram A.F.J. & Meyer V. (2016), Highly active promoters and native secretion signals for protein production during extremely low growth rates in Aspergillus niger, Microbial Cell Factories 15: 145.
- Patyshakuliyeva A., Arentshorst M., Allijn I.E., Ram A.F.J., Vries R.P. de & Gelber I.B. (2016), Improving cellulase production by Aspergillus niger using adaptive evolution, Biotechnology Letters 38(6): 969-974.
- Niu J., Arentshorst M., Seelinger F., Ram A.F.J. & Ouedraogo J.P. (2016), A set of isogenic auxotrophic strains for constructing multiple gene deletion mutants and parasexual crossings in Aspergillus niger, Archives of Microbiology 198(9): 861-868.
- Ouedraogo J., Arentshorst M., Nikolaev I., Barends S. & Ram A.F.J. (2016), I-Scel enzyme mediated integration (SEMI) for fast and efficient gene targeting in Trichoderma reesei, Journal of Biotechnology 222: 25-28.
- Rocha M.C., Tadini M.F.J.H., Godoy K.F. de, Castro P.A. de, Hori J.I., Cunha A.F. da, Arentshorst M., Ram A.F.J., Hondel C.A.M.J.J. van den, Goldman G.H. & Malavazi I. (2016), Aspergillus fumigatus MADS-Box Transcription Factor rlmA Is Required for Regulation of the Cell Wall Integrity and Virulence, G3: Genes | Genomes | Genetics 6(9): 2983-3002.
- Niu J., Arentshorst M., Nair P.D., Dai Z., Baker S., Frisvad J.C., Nielsen K.F., Punt P.J. & Ram A.F.J. (2016), Identification of a classical mutant in the industrial host Aspergillus niger by systems genetics: LaeA Is required for citric acid production and regulates the formation of some secondary metabolites.G3 (Bethesda), G3: Genes | Genomes | Genetics 6(1): 193-204.
- Park J., Hulsman M., Arentshorst M., Breeman M., Alazi E., Lagendijk E.L., Rocha M.C., Malavazi I., Nitsche B.M., Hondel C.A.M.J.J. van den, Meyer V. & Ram A.F.J. (2016), Transcriptomic and molecular genetic analysis of the cell wall salvage response of Aspergillus niger to the absence of galactofuranose synthesis, Cellular Microbiology 18(9): 1268-1284.
- Ouedraogo J.P., Arentshorst M., Nikolaev I., Barends S. & Ram A.F.J. (2015), I-SceI-mediated double-strand DNA breaks stimulate efficient gene targeting in the industrial fungus Trichoderma reesei, Applied Microbiology and Biotechnology 99(23): 10083-10095.
- Arentshorst M., Lagendijk E.L. & Ram A.F.J. (2015), A new vector for efficient gene targeting to the pyrG locus in Aspergillus niger, Fungal Biology and Biotechnology 2: 2.
- Arenthorst M., Jing N. & Ram A.F.J. (2015), Efficient generation of Aspergillus niger knock out strains by combining NHEJ mutants and a split Marker Approach. In: Berg M.A. van den & Maruthachalam K. (red.), Genetic Transformation Systems in Fungi. Fungal Biology nr. 1. Cham: Springer. 263-272.
- Kwon M.J., Arentshorst M., Fiedler M., Groen F.L. de, Punt P.J., Meyer V. & Ram A.F.J. (2014), Molecular genetic analysis of vesicular transport in Aspergillus niger reveals partial conservation of the molecular mechanism of exocytosis in fungi, Microbiology 160(14-01): 316-329.
- Park J.P., Tefsen B., Arentshorst M., Lagendijk E., Hondel C.A.M.J.J. van den, Die I. van & Ram A.F.J. (2014), Identification of the UDP-glucose-4-epimerase required for galactofuranose biosynthesis and galactose metabolism in A. niger, Fungal Biology and Biotechnology 1: 6.
- Arentshorst M., Jing N. & Ram A.F.J. (2014), Efficient Generation of Aspergillus niger Knock Out Strains by Combining NHEJ Mutants and a Split Marker Approach. Genetic Transformation Systems in Fungi. In: , Genetic Transformation Systems in Fungi I.
- Kwon M.J., Nitsche B.M., Arentshorst M., Jørgensen T.R., Ram A.F.J. & Meyer V. (2013), The transcriptomic signature of RacA activation and inactivation provides new insights into the morphogenetic network of Aspergillus niger, PLoS ONE 8(7): e68946.
- Jørgensen T.R., Park J.-H., Arentshorst M., Welzen A.M. van, Lamers G.E.M., VanKuyk P.A., Damveld R.A., Hondel C.A.M.J.J. van den, Nielsen K.F., Frisvad J.C. & Ram A.F.J. (2011), The molecular and genetic basis of conidial pigmentation in Aspergillus niger, Fungal Genetics and Biology 48(5): 544-553.