Bobby Florea
Assistant Professor
- Name
- Dr. B.I. Florea
- Telephone
- +31 71 527 4342
- b.florea@chem.leidenuniv.nl
- ORCID iD
- 0000-0001-7114-2266
More information about Bobby Florea
News
-
From discovery to business: 'In the lab, we often don't realise that we are working to help an immense number of patients' -
Iprotics Wins the Venture Challenge Spring 2022 -
From lab discovery to a new drug: the Venture Challenge makes it possible -
European collaboration shines a light on enzyme discovery for industry
Teaching
Curriculum Vitae
Personal information
Nationality: Dutch
Date of birth: 14 October 1973
Education
2003 PhD, Leiden Amsterdam Center for Drug Research, Leiden University, The Netherlands. Thesis title: “Polymeric carriers for drug and gene delivery”
Promotors: Prof. G. Borchard and Prof. H.E. Junginger
1998 MSc in Bio Pharmaceutical Sciences (BFW), Leiden Amsterdam Centre for Drug Research, Leiden University, The Netherlands
1995 Propedeuse in Chemistry, Leiden Institute for Chemistry, Leiden University, The Netherlands
Current and previous research positions
2022 – now Assistant Professor and Executive Officer Chemical Proteomics Reagens, Leiden Institute for Chemistry, Leiden University, The Netherlands
2010 – 2022 Scientist and Executive Officer Chemical Proteomics Reagens, Leiden Institute of Chemistry, Leiden University, The Netherlands
2004 – 2010 Postdoctoral fellow, Leiden Institute for Chemistry, Leiden University, The Netherlands (Prof. H.S. Overkleeft)
2002 – 2004 Postdoctoral fellow, Cell Biology and Genetics Erasmus Medical Center, Erasmus University (Prof. J.H.J Hoeijmakers)
1997 – 1998 Student assistant, Ciba Geigy (Novartis), Basel, Switzerland (Prof. T. Arvinte)
Contribution to teaching and supervision
- Chemical Analysis Methods course for 1st year BSc Molecular Science and Technology at Leiden University:
- Guest lecturer
- 2012 – 2018 Chemical Genetics master class (Prof. J. Brouwer)
- 2013 – 2019 Honour class (Prof. M. Noteborn)
- 2014 – 2017 Netherlands Research School Chemical Biology (Prof. J. Brouwer)
Co-promoter of 3 PhD theses, co-supervision 9 PhD students, supervision of 22 MSc and 71 BSc students since 2004.
Other current responsibilities since 2016
- Editor of the book: Activity-Based Proteomics, Methods in Biology, 2017, vol 1491, editors HS Overkleeft and BI Florea, Humana New York, NY. DOI: 10.1007/978-1-4939-6439-0
- Organizer and Chair of the 2017 European Activity based proteomics meeting in Leiden
- Reviewer for 21 international journals and 3 European funding agencies
- Executive Officer Chemical Proteomics Reagents
- Setup and daily operation of the high-resolution mass spectrometry expertise lab of the Leiden Institute for Chemistry, Leiden University
- Setup of protein analysis and quantification methods and training of young researchers in these
- Co-supervisor and co-manager of the Dutch Compound Library repository of the Leiden Institute for Chemistry, Leiden University
Publication record including five recent representative publications
115 publications with 2600 citations, H-index 37 (Web of Science)
Full publication record: Leiden University
- Besse L, et al., Florea BI, Overkleeft HS, Driessen C, Treatment with HIV-protease inhibitor Nelfinavir identifies membrane lipid composition & fluidity as therapeutic target in advanced multiple myeloma. Cancer Res. 2021, 81(17): 4581-4593. DOI: 10.1158/0008-5472.CAN-20-3323
- Inagawa T, et al., Florea BI, Verkaik NS, van Gent DC, C-terminal extensions of Ku70 and Ku80 differentially influence DNA end binding properties, Int. J. Molec. Sci. 2020, 21(18): 6725. DOI: 10.3390/ijms21186725
- Armstrong Z, et al., Florea BI, Overkleeft HS, Davies GJ, Manno-epi-cyclophellitols enable activity-based protein profiling of human α-mannosidases and discovery of new golgi mannosidase II inhibitors, J. A. C. S. 2020, 142(30): 13021-13029. DOI: 10.1021/jacs.0c03880
- Rooden EJ, Florea BI, et al., Aerts JMFG, van der Stelt M, Chemical proteomic analysis of serine hydrolase activity in Niemann-Pick type C mouse brain, Front. Neurosci. 2018, 12: 440. DOI: 10.3389/fnins.2018.00440
- de Bruin G. de, Xin BT, Florea BI, Overkleeft HS, Proteasome subunit selective activity-based probes report on proteasome core particle composition in a native polyacrylamide gel electrophoresis fluorescence-resonance energy transfer assay, J. A. C. S. 2016, 138(31): 9874-9880. DOI: 10.1021/jacs.6b04207
Assistant Professor
- Science
- Leiden Institute of Chemistry
- LIC/Chemical Biology
- LIC/CB/Bio-organic Synthesis
- Bakker A.T., Kotsogianni A.I., Mirenda L., Straub V.M., Avalos Garcia M., Berg R.J.B.H.N. van den, Florea B.I., Wezel G.P. van, Janssen A.P.A., Martin N.I. & Stelt M. van der (2023), Chemical proteomics reveals antibiotic targets of oxadiazolones in MRSA, Journal of the American Chemical Society 145(2): 1136-1143.
- Jiang M., Huizenga M.C.W., Wirth J.L., Paloczi J., Amedi A., Berg R.J.B.H.N. van den, Benz J., Collin L., Deng H., Di X., Driver W.F., Florea B.I., Grether U., Janssen A.P.A., Hankemeier T., Heitman L.H., Lam T.W., Mohr F., Pavlovic A., Ruf I., Hurk H. van den, Stevens A.F., Vliet D. van der, Wel T. van der, Wittwer M.B., Boeckel C.A.A. van, Pacher P., Hohmann A.G. & Stelt M. van der (2023), A monoacylglycerol lipase inhibitor showing therapeutic efficacy in mice without central side effects or dependence, Nature Communications 14(1): 8039.
- Reichelt J., Sachs W., Frombling S., Fehlert J., Studencka-Turski M., Betz A., Loreth D., Blume L., Witt S., Pohl S., Brand J., Czesla M., Knop J., Florea B.I., Zielinski S., Sachs M., Hoxha E., Hermans-Borgmeyer I., Zahner G., Wiech T., Kruger E. & Meyer-Schwesinger C. (2023), Non-functional ubiquitin C-terminal hydrolase L1 drives podocyte injury through impairing proteasomes in autoimmune glomerulonephritis, Nature Communications 14(1): 2114.
- Kuo C.L., Su Q., Nieuwendijk A.M.C.H. van den, Beenakker T.J.M., Offen W.A., Willems L.I., Boot R.G., Sarris A.J.C., Rosa Alcalde Marques A.R.A., Codée J.D.C., Marel G.A. van der, Florea B.I., Davies G.J., Overkleeft H.S. & Aerts J.M.F.G. (2023), The development of a broad-spectrum retaining β-exo-galactosidase activity-based probe, Organic and Biomolecular Chemistry 21(38): 7813-7820.
- Zhou X., Besse A., Peter J., Steinhardt M.J., Vogt C., Nerreter S., Teufel E., Stanojkovska E., Xiao X., Hornburger H., Haertle L., Lopez M.M., Munawar U., Riedel A., Han S., Maurits E., Overkleeft H.S., Florea B.I., Einsele H., Kortüm K.M., Driessen C., Besse L. & Rasche L. (2023), High-dose carfilzomib achieves superior anti-tumor activity over lowdose and recaptures response in relapsed/refractory multiple myeloma resistant to low-dose carfilzomib by co-inhibiting the β2 and β1 subunits of the proteasome complex, Haematologica 108(6): 1628-1639.
- Leeuwen T. van, Doelman W., Kieboom R.W.R. van den, Florea B.I. & Kasteren S.I. van (2023), Bioorthogonal peptide enrichment from complex samples using a rink-amide-based catch-and-release strategy, ChemBioChem 24(8): e202300082.
- Bakker A.T., Kotsogianni A.I., Mirenda L., Straub V.M., Avalos Garcia M., Berg R.J.B.H.N. van den, Florea B.I., Wezel G.P. van, Janssen A.P.A., Martin N.I. & Stelt M. van der (2022), Chemical proteomics reveals antibiotic targets of oxadiazolones in MRSA, Journal of the American Chemical Society 145(2): 1136-1143.
- McGregor N.G.S., Kuo C.L., Beenakker T.J.M., Wong C.S., Offen W.A., Armstrong Z.W.B., Florea B.I., Codee J.D.C., Overkleeft H.S., Aerts J.M.F.G. & Davies G.J. (2022), Synthesis of broad-specificity activity-based probes for exo-beta-mannosidases, Organic and Biomolecular Chemistry 20(4): 877-886.
- Gagestein B., Stevens A.F., Fazio D., Florea B.I., Wel T. van der, Bakker A.T., Fezza F., Dulk J. den, Overkleeft H.S., Maccarrone M. & Stelt M. van der (2022), Chemical proteomics reveals off-targets of the anandamide reuptake inhibitor WOBE437, ACS Chemical Biology 17(5): 1174-1183.
- Beerkens B.L.H., Koc C., Liu R., Florea B.I., Le Dévédec S.E., Heitman L.H., IJzerman A.P. & Es D. van der (2022), A chemical biological approach to study G protein-coupled receptors: labeling the adenosine A1 receptor using an electrophilic covalent probe, ACS Chemical Biology 17(11): 3131-3139.
- Gagestein B., von Hegedus J.H., Kwekkeboom J.C., Heijink M., Blomberg N., Wel T. van der, Florea B.I., Elst H. van der, Wals K., Overkleeft H.S., Giera M., Toes R.E.M., Ioan-Facsinay A. & Stelt M. van der (2022), Comparative photoaffinity profiling of omega-3 signaling lipid probes reveals prostaglandin reductase 1 as a metabolic hub in human macrophages, Journal of the American Chemical Society 144(41): 18938–18947.
- Boer C. de, McGregor N.G.S., Peterse E., Schröder S.P., Florea B.I., Jiang J., Reijngoud J., Ram A.F.J., Wezel G.P. van, Marel G.A. van der, Codee J.D.C., Overkleeft H.S. & Davies G.J. (2021), Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases, RSC Chemical Biology 2(6): 1701.
- Mendez M., Andrej B., Florea B.I., Christian Z., Overkleeft H.S., Lenka B. & Driessen C. (2021), Carfilzomib-induced acute cardiotoxicity is mediated through angiotensin and caused by cardiomyocyte energy depletion, Clinical Lymphoma, Myeloma and Leukemia 21(2): S122.
- Marcoux G., Laroche A., Hasse S., Bellio M., Mbarik M., Tamagne M., Allaeys I., Zufferey A., Lévesque T., Rebetz J., Karakeussian-Rimbaud A., Turgeon J., Bourgoin S.G., Hamzeh-Cognasse H., Cognasse F., Kapur R., Semple J.W., Hebert M.J., Pirenne F., Overkleeft H.S., Florea B.I., Dieude M., Vingert B. & Boilard E. (2021), Platelet EVs contain an active proteasome involved in protein processing for antigen presentation via MHC-I molecules, Blood 138(25): 2607-2620.
- Schroder S.P., Offen W.A., Males A., Jin Y., Boer C. de, Enotarpi J., Marino L., Marel G.A. van der, Florea B.I., Codee J.D.C., Overkleeft H.S. & Davies G.J. (2021), Development of Non-Hydrolysable Oligosaccharide Activity-Based Inactivators for Endoglycanases: A Case Study on alpha-1,6 Mannanases, Chemistry: a European Journal 27(37): 9519-9523.
- Besse L., Besse A., Stolze S.C., Sobh A., Zaal E.A., Ham A.J. van der, Ruiz M., Phuyal S., Büchler L., Sathianathan M., Florea B.I., Borén J., Ståhlman M., Huber J., Bolomsky A., Ludwig H., Hannich J.T., Loguinov A., Everts B., Berkers C.R., Pilon M., Farhan H., Vulpe C.D., Overkleeft H.S. & Driessen C. (2021), Treatment with HIV-protease inhibitor nelfinavir identifies membrane lipid composition and fluidity as a therapeutic target in advanced multiple myeloma, Cancer Research 81(17): 4581-4593.
- Chen Y., Armstrong Z., Artola M., Florea B.I., Kuo C.L., Boer C. de, Rasmussen M.S., Abou Hachem M., Marel G.A. van der, Codee J.D.C., Aerts J.M.F.G., Davies G.J. & Overkleeft H.S. (2021), Activity-based protein profiling of retaining alpha-amylases in complex biological samples, Journal of the American Chemical Society 143(5): 2423-2432.
- Rayo J., Gregor R., Jacob N.T., Dandela R., Dubinsky L., Yashkin A., Aranovich A., Thangaraj M., Ernst O., Barash E., Malitsky S., Florea B.I., Krom B.P., Wiemer E.A.C., Kickhoefer V.A., Rome L.H., Mathison J.C., Kaufmann G.F., Overkleeft H.S., Cravatt B.F., Zor T., Janda K.D., Ulevitch R.J., Kravchenko V.V. & Meijler M.M. (2021), Immunoediting role for major vault protein in apoptotic signaling induced by bacterial N-acyl homoserine lactones, Proceedings of the National Academy of Sciences 118(12 ): e2012529118.
- Leeuwen T. van, Araman C., Pournara L.P., Kampstra A.S.B., Bakkum T., Marqvorsen M.H.S., Nascimento C.R., Groenewold G.J.M., Wulp W. van der, Camps M.G.M., Janssen G.M.C., Veelen P.A. van, Westen G.J.P. van Janssen A.P.A., Florea B.I., Overkleeft H.S., Ossendorp F.A., Toes R.E.M. & Kasteren S.I. van (2021), Bioorthogonal protein labelling enables the study of antigen processing of citrullinated and carbamylated auto-antigens, RSC Chemical Biology 2(3): 855-862.
- Voorneveld J., Florea B.I., Bakkum T., Mendowicz R.J., Veer M.S. van der, Gagestein B., Kasteren S.I. van, Stelt M. van der, Overkleeft H.S. & Filippov D.V. (2020), Olaparib-Based Photoaffinity Probes for PARP-1 Detection in Living Cells, ChemBioChem 21: 1-5.
- Selkrig J., Li N., Hausmann A., Mangan M.S.J., Zietek M., Mateus A., Bobonis J., Sueki A., Imamura H., El Debs B., Sigismondo G., Florea B.I., Overkleeft H.S., Kopitar-Jerala N., Turk B., Beltrao P., Savitski M.M., Latz E., Hardt W.D., Krijgsveld J. & Typas A. (2020), Spatiotemporal proteomics uncovers cathepsin-dependent macrophage cell death during Salmonella infection, Nature Microbiology 5: 1119-1133.
- Overkleeft H.S., Xin B.T., Espinal C.G.A., Bruin G. de, Filippov D.V., Marel G.A. van der & Florea B.I. (2020), Two-step bioorthogonal activity-based protein profiling of individual human proteasome catalytic sites, ChemBioChem 21(1-2): 248-255.
- Maurits E., Graaff M.J. van de, Maiorana S., Wander D.P.A., Dekker P.M., Zanden S.Y. van der, Florea B.I., Neefjes J.J.C., Overkleeft H.S. & Kasteren S.I. van (2020), Immunoproteasome inhibitor-doxorubicin conjugates target multiple myeloma cells and release doxorubicin upon low-dose photon irradiation, Journal of the American Chemical Society 142(16): 7250-7253.
- Pattipeiluhu R., Crielaard S., Klein-Schiphorst I., Florea B.I., Kros A. & Campbell F. (2020), Unbiased identification of the liposome protein corona using photoaffinity-based chemoproteomics, ACS Central Science 2020(6): 535-545.
- Armstrong Z., Kuo C.L., Lahav D., Liu B., Johnson R., Beenakker T.J.M., Boer C. de, Wong C.S., Rijssel E.R. van, Debets M.F., Florea B.I., Hissink C., Boot R.G., Geurink P.P., Ovaa H., Stelt M. van der, Marel G.A. van der, Codée J.D.C., Aerts J.M.F.G., Wu L., Overkleeft H.S. & Davies G.J. (2020), Manno-epi-cyclophellitols enable activity-based protein profiling of human α-mannosidases and discovery of new golgi mannosidase II inhibitors, Journal of the American Chemical Society 142(30): 13021-13029.
- Mock E.D., Mustafa M., Gunduz-Cinar O., Cinar R., Petrie G.N., Kantae V., Di X., Ogasawara D., Varga Z.V., Paloczi J., Miliano C., Donvito G., Esbroeck A.C.M. van, Gracht A.M.F. van der, Kotsogianni I., Park J.K., Martella A., Wel T. van der, Soethoudt M., Jiang M., Wendel T.J., Janssen A.P.A., Bakker A.T., Donovan C.M., Castillo L.I., Florea B.I., Wat J., Hurk H. van den, Wittwer M., Grether U., Holmes A., Boeckel C.A.A. van, Hankemeier T., Cravatt B.F., Buczynski M.W., Hill M.N., Pacher P., Lichtman A.H. & Stelt M. van der (2020), Discovery of a NAPE-PLD inhibitor that modulates emotional behavior in mice, Nature Chemical Biology 16(6): 667-675.
- Wel T. van der Hilhorst R Dulk H. den Hooven T. van den Prins N.M. Wijnakker J.A.P.M. Florea B.I. Lenselink E.B. Westen G.J.P. van Ruijtenbeek R. Overkleeft H.S. Kaptein A. Barf T. Stelt M. van der (2020), Chemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis, Nature Communications 11(1): 3216.
- Maurits E. Degeling C.G. Kisselev A.F. Florea B.I. Overkleeft H.S. (2020), Structure-based design of fluorogenic substrates selective for human proteasome subunits, ChemBioChem 21(22): 3220-3224.
- Koenders S.T.A., Rooden E.J. van, Elst H. van den, Florea B.I., Overkleeft H.S. & Stelt M. van der (2020), STA-55, an Easily Accessible, Broad-Spectrum, Activity-Based Aldehyde Dehydrogenase Probe, ChemBioChem 21: 1911-1917.
- Inagawa T., Wennink T., Lebbink J.H.G., Keijzers G., Florea B.I., Verkaik N.S. & Gent D.C. van (2020), C-terminal extensions of Ku70 and Ku80 differentially influence DNA end binding properties, International Journal of Molecular Sciences 21(18): 6725.
- Besse A., Besse L., Kraus M., Mendez-Lopez M., Bader J., Xin B., Bruin G. de, Maurits E., Overkleeft H.S. & Driessen C. (2019), Proteasome inhibition in multiple myeloma: head-to-head comparison of currently available proteasome inhibitors, Cell chemical biology 26(3): 340-351.
- Koenders S.T.A., Wijaya L.S., Erkelens M.N., Bakker A.T., Noord V.E. van der, Rooden E.J. van, Burggraaff L., Putter P.C., Botter E., Wals K., Elst H. van den, Dulk H. den, Florea B.I., Water B. van de, Westen G.J.P. van, Mebius R.E., Overkleeft H.S., Le Dévédec S.E. & Stelt M. van der (2019), Development of a Retinal-Based Probe for the Profiling of Retinaldehyde Dehydrogenases in Cancer Cells, ACS Central Science 5(12): 1965-1974.
- Mendez M., Besse A., Besse L., Florea B.I., Christian Z., Overkleeft H.S. & Driessen C. (2019), Proteasome inhibition by Carfilzomib, but not by Bortezomib, induces a specific, proteasome subunit-dependent impairment in cardiac contractility, Clinical Lymphoma, Myeloma and Leukemia 19(10): E119.
- Baggelaar M.P., Dulk H. den, Florea B.I., Fazio D., Bernab Ograve N., Raspa M., Janssen A.P.A., Scavizzi F., Barboni B., Overkleeft H.S., Maccarrone M. & Stelt M. van der (2019), ABHD2 inhibitor identified by activity-based protein profiling reduces acrosome reaction, ACS Chemical Biology 14(10): 2295-2304.
- Moiola M., Bova A., Crespi S., Memeo M.G., Mella M., Overkleeft H.S., Florea B.I. & Quadrelli P. (2019), Fluorescent Probes from Aromatic Polycyclic Nitrile Oxides: Isoxazoles versus Dihydro-1 lambda(3),3,2 lambda(4)-Oxazaborinines, ChemistryOpen 8(6): 770-780.
- Moiola M., Crespi S., Memeo M., Collina S., Overkleeft H.S., Florea B.I. & Quadrelli P. (2019), Scope and Limitations of Boron Fluorescent Complexes from Stable Nitrile Oxides in ABPP Assays, ACS Omega 4(4): 7766-7774.
- Nykiel M., Lisik P., Debski J., Florea B.I. & Rybka K. (2019), Chl a fluorescence and proteomics reveal protection of the photosynthetic apparatus to dehydration in tolerant but not in susceptible wheat cultivars, Biologia Plantarum 63(1): 287-297.
- Zhou J., Mock E.D., Martella A., Kantae V., Di X., Burggraaff L., Baggelaar M.P., Al-Ayed K., Bakker A., Florea B.I., Grimm S.H., Dulk H. den, Li C.T., Mulder L., Overkleeft H.S., Westen G.J.P. van, Hankemeier T. & Stelt M. van der (2019), Activity-based protein profiling identifies α-ketoamides as inhibitors for Phospholipase A2 Group XVI, ACS Chemical Biology 14(2): 164-169.
- Steverding D., Florea B.I. & Overkleeft H.S. (2019), Trypanosoma brucei: β2-selective proteasome inhibitors do not block the proteasomal trypsin-like activity but are trypanocidal, Molecular and Biochemical Parasitology 227: 1-4.
- Xin B.T., Huber E.M., Bruin G. de, Heinemeyer W., Maurits E., Espinal C., Du Y., Janssens M., Weyburne E.S., Kisselev A.F., Florea B.I., Driessen C., Marel G.A., Groll M. & Overkleeft H.S. (2019), Structure-Based Design of Inhibitors Selective for Human Proteasome β2c or β2i Subunits, Journal of Medicinal Chemistry 62(3): 1626-1642.
- Schröder S.P., Boer C. de, McGregor N.G.S., Rowland R.J., Moroz O., Blagova E., Reijngoud J., Arentshorst M., Osborn D., Morant M.D., Abbate E., Stringer M.A., Krogh K.B.R.M., Raich L., Rovira C., Wezel G.P. van, Berrin J.G., Florea B.I., Ram A.F.J., Codée J.D.C., Marel G.A. van der, Wu L., Wilson K.S., Overkleeft H.S. & Davies G.J. (2019), Dynamic and functional profiling of Xylan-degrading enzymes in aspergillus secretomes using activity-based probes, ACS Central Science 5(6): 1067-1078.
- Schipper-Krom S., Sanz A.S., Bodegraven E.J. van, Speijer D., Florea B.I., Ovaa H. & Reits E.A. (2019), Visualizing Proteasome Activity and Intracellular Localization Using Fluorescent Proteins and Activity-Based Probes, Frontiers in Molecular Biosciences 6: 56.
- Skorda A., Sklirou A.D., Sakellaropoulos T., Gianniou D.D., Kastritis E., Terpos E., Tsitsilonis O.E., Florea B.I., Overkleeft H.S., Dimopoulos M.A., Alexopoulos L.G. & Trougakos I.P. (2019), Non-lethal proteasome inhibition activates pro-tumorigenic pathways in multiple myeloma cells, Journal of Cellular and Molecular Medicine 23(12): 8010-8018.
- Steverding D., Rushworth S.A., Florea B.I. & Overkleeft H.S. (2019), Trypanosoma brucei: Inhibition of cathepsin L is sufficient to kill bloodstream forms, Molecular and Biochemical Parasitology 235: 111246.
- Esbroeck A.C.M. van, Kantae V., Di X., Wel T. van der, Dulk J. den, Stevens A.F., Singh S., Bakker A.T., Florea B.I., Stella N., Overkleeft H.S., Hankemeier T. & Stelt M. van der (2019), Identification of alpha,beta-Hydrolase Domain Containing Protein 6 as a Diacylglycerol Lipase in Neuro-2a Cells, Frontiers in Molecular Neuroscience 12: 286.
- Esbroeck A.C.M. van, Kantae V., Di X., Wel T. van der, Dulk J. den, Stevens A.F., Singh S., Bakker A.T., Florea B.I., Stella N., Overkleeft H.S., Hankemeier T. & Stelt M. van der (2019), Identification of α,β-Hydrolase Domain Containing Protein 6 as a Diacylglycerol Lipase in Neuro-2a Cells, Frontiers in Molecular Neuroscience 12: 286.
- Artola M., Kuo C.L., McMahon S.A., Oehler V., Hansen T., Lienden M.J.C. van der, He X., Elst H. van den, Florea B.I., Kermode A.R., Marel G.A. van der, Gloster T.M., Codée J.D.C., Overkleeft H.S. & Aerts J.M.F.G. (2018), New Irreversible alpha-l-Iduronidase Inhibitors and Activity-Based Probes, Chemistry: a European Journal 24(71): 19081-19088.
- Rooden E.J. van, Esbroeck A.C.M. van, Baggelaar M.P., Deng H., Florea B.I., Rosa Alcalde Marques A., Ottenhoff R., Boot R.G., Overkleeft H.S., Aerts J.M.F.G. & Stelt M. van der (2018), Chemical Proteomic Analysis of Serine Hydrolase Activity in Niemann-Pick Type C Mouse Brain, Frontiers in Neuroscience 12: 440.
- Eising S., Xin B., Kleinpenning F., Heming J.J.A., Florea B.I., Overkleeft H.S. & Bonger K.M. (2018), Coordination-Assisted Bioorthogonal Chemistry: Orthogonal Tetrazine Ligation with Vinylboronic Acid and a Strained Alkene, ChemBioChem 19(15): 1648-1652.
- Downey-Kopyscinski S., Daily E.W., Gautier M., Bhatt A., Florea B.I., Mitsiades C.S., Richardson P.G., Driessen C., Overkleeft H.S. & Kisselev A.F. (2018), An inhibitor of proteasome beta 2 sites sensitizes myeloma cells to immunoproteasome inhibitors, Blood Advances 2(19): 2443-2451.
- Rooden E.J. van, Florea B.I., Deng H., Baggelaar M.P., Esbroeck A.C.M. van, Zhou J., Overkleeft H.S. & Stelt M. van der (2018), Mapping in vivo target interaction profiles of covalent inhibitors using chemical proteomics with label-free quantification, Nature Protocols 13(4): 752-767.
- Kytidou K., Beekwilder J., Artola M., Meel E. van, Wilbers R.H.P., Moolenaar G.F., Goosen N., Ferraz M.J., Katzy R., Voskamp P., Florea B.I., Hokke C.H., Overkleeft H.S., Schots A., Bosch D., Pannu N. & Aerts J.M.F.G. (2018), Nicotiana benthamiana α-galactosidase A1.1 can functionally complement human α-galactosidase A deficiency associated with Fabry disease, Journal of Biological Chemistry 293(26): 10042-10058.
- Bruin G. de, Rooden E.J. van, Ward D., Wesseling C.M.J., Nieuwendijk A.M.C.H. van den, Boeckel C.A.A. van, Driessen C., Kisselev A.F., Florea B.I., Stelt M. van der & Overkleeft H.S. (2017), Asymmetric synthesis of lysine analogues with reduced basicity, and their incorporation into proteasome inhibitors, European Journal of Organic Chemistry 2017(39): 5921-5934.
- Raz V., Raz Y., Paniagua-Soriano G., Roorda J.C., Olie C., Riaz M. & Florea B.I. (2017), Proteasomal activity-based probes mark protein homeostasis in muscles, Journal of Cachexia, Sarcopenia and Muscle 8(5): 798-807.
- Overkleeft H.S. & Florea B.I. (2017), Activity-Based Proteomics: Methods and Protocols. Methods in Molecular Biology no. 1491. New York: Humana Press.
- Jiang J., Kuo C.L., Wu L., Franke C., Kallemeijn W.W., Florea B.I., Meel H.M.M. van, Marel G.A. van der, Codee J.D.C., Boot R.G., Davies G.J., Overkleeft H.S. & Aerts J.M.F.G. (2017), Correction to "Detection of Active Mammalian GH31 α-Glucosidases in Health and Disease Using In-Class, Broad-Spectrum Activity-Based Probes", ACS Central Science 3(6): 673.
- Wu L., Jiang J., Jin Y., Kallemeijn W.W., Kuo C.-L., Artola M., Dai W., Elk C. van, Eijk M. van, Marel G.A. van der, Codee J.D.C., Florea B.I., Aerts J.M.F.G., Overkleeft H.S. & Davies G.J. (2017), Activity-based probes for functional interrogation of retaining beta-glucuronidases, Nature Chemical Biology 13(8): 867-873.
- Liu Q., Florea B.I. & Filippov D.V. (2017), ADP-Ribosylation Goes Normal: Serine as the Major Site of the Modification, Cell chemical biology 24(4): 431-432.
- Tsakiri E.N., Terpos E., Papanagnou E.D., Kastritis E., Brieudes V., Halabalaki M., Bagratuni T., Florea B.I., Overkleeft H.S., Scorrano L., Skaltsounis A.L., Dimopoulos M.A. & Trougakos I.P. (2017), Milder degenerative effects of Carfilzomib &ITvs&IT. Bortezomib in the Drosophila model: a link to clinical adverse events, Scientific Reports 7: 17802.
- Schröder S.P., Sande J.W. van de, Kallemeijn W.W., Kuo C.L., Artola Perez de Azanza M.E., Rooden E.J. van, Jiang J., Beenakker T.J.M., Florea B.I., Offen W.A., Davies G.J., Minnaard A.J., Aerts J.M.F.G., Codee J.D.C., Marel G.A. van der & Overkleeft H.S. (2017), Towards broad spectrum activity-based glycosidase probes: synthesis and evaluation of deoxygenated cyclophellitol aziridines, Chemical Communications 53(93): 12528-12531.
- Elings W., Tassoni R., Schoot S.A. van der, Luu W., Kynast J.P., Dai L., Blok A.J., Timmer M., Florea B.I., Pannu N.S. & Ubbink M. (2017), Phosphate Promotes the Recovery of Mycobacterium tuberculosis beta-Lactamase from Clavulanic Acid Inhibition, Biochemistry 56(47): 6257-6267.
- Ben Bdira F., Kallemeijn W.W., Oussoren S.V., Scheij S., Bleijlevens B., Florea B.I., Roomen C.P.A.A. van, Ottenhoff R., Kooten M.J.F.M. van, Walvoort M.T.C., Witte M.D., Boot R.G., Ubbink M., Overkleeft H.S. & Aerts J.M.F.G. (2017), Stabilization of Glucocerebrosidase by Active Site Occupancy, ACS Chemical Biology 12(7): 1830-1841.
- Baggelaar M.P., Esbroeck A.C.M. van, Rooden E.J. van, Florea B.I., Overkleeft H.S., Marsicano G., Chaouloff F. & Stelt M. van der (2017), Chemical Proteomics Maps Brain Region Specific Activity of Endocannabinoid Hydrolases, ACS Chemical Biology 12(3): 852-861.
- Marques A.R., Willems L.I., Herrera Moro D., Florea B.I., Scheij S., Ottenhof R., Roomen C. van, Verhoek M., Nelson J.K., Kallemeijn W.W., Biela-Banas A., Martin O.R., Cachón-González M.B., Kim N.N., Cox T.M., Boot R.G., Overkleeft H.S. & Aerts J.M.F.G. (2017), A specific activity-based probe to monitor family GH59 galactosylceramidase - the enzyme deficient in Krabbe disease, ChemBioChem 18(4): 402-412.
- Esbroeck A.C.M. van, Janssen A.P.A., Cognetta III A.B., Ogasawara D., Shpak G., Kroeg M. van der, Kantae V., Baggelaar M.P., Vrij F.M.S. de, Deng H., Allara M., Fezza F., Lin Z., Wel T. van der, Soethoudt M., Mock E.D., Dulk H. den, Baak I.L., Florea B.I., Hendriks G., Petrocellis L. de, Overkleeft H.S., Hankemeier T., Zeeuw C.I. de, Di Marzo V., Maccarrone M., Cravatt B.F., Kushner S.A. & Stelt M. van der (2017), Activity-based protein profiling reveals off-target proteins of the FAAH inhibitor BIA 10-2474, Science 356(6342): 1084-1087.
- Baker Y.R., Hodgkinson J.T., Florea B.I., Alza E., Galloway W.R.J.D., Grimm L., Geddis S.M., Overkleeft H.S., Welch M. & Spring D.R. (2017), Identification of new quorum sensing autoinducer binding partners in Pseudomonas aeruginosa using photoaffinity probes, Chemical Science 8(11): 7403-7411.
- Gubbens J., Wu C., Zhu H., Filippov D.V., Florea B.I., Rigali S., Overkleeft H.S. & Wezel G.P. van (2017), Intertwined Precursor Supply during Biosynthesis of the Catecholate-Hydroxamate Siderophores Qinichelins in Streptomyces sp MBT76, ACS Chemical Biology 12(11): 2756-2766.
- Kasteren S.I. van, Florea B.I. & Overkleeft H.S. (2017), Activity-Based Protein Profiling: From Chemical Novelty to Biomedical Stalwart. In: Florea B.I. & Overkleeft H.S. (Eds.), Activity-based Proteomics: Methods and Protocols. Methods in Molecular Biology. New York: Humana Press. 1-8.
- Widjaja C.E., Olvera J.G., Metz P.J., Phan A.T., Savas J.N., Bruin G. de, Leestemaker Y., Berkers C.R., Jong A. de, Florea B.I., Fisch K., Lopez J., Kim S.H., Garcia D.A., Searles S., Bui J.D., Chang A.N., Yates J.R.III, Goldrath A.W., Overkleeft H.S., Ovaa H. & Chang J.T. (2017), Proteasome activity regulates CD8(+) T lymphocyte metabolism and fate specification, Journal of Clinical Investigation 127(10): 3609-3623.
- Paniagua Soriano G., Overkleeft H.S. & Florea B.I. (2017), Two-Step Activity-Based Protein Profiling with the Proteasome System as Model of Study. In: Overkleeft H.S. & Florea B.I. (Eds.), Activity-based Proteomics: Methods and Protocols. Methods in Molecular Biology no. 1491. New York: Humana Press. 205-215.
- Baumann T., Vosyka O., Florea B.I., Overkleeft H.S., Meiners S. & Kammerl I.E. (2016), Activity-based Pull-down of Proteolytic Standard and Immunoproteasome Subunits, Bio-protocol 6(24): e2073.
- Widjaja C.E., Metz P.J., Bruin G. de, Leestemaker Y., Savas J.N., Phan A.T., Goldrath A.W., Yates J.R., Florea B.I., Overkleeft H.S., Ovaa H. & Chang J.T. (2016), Proteasome degradative activity regulates CD8(+) T lymphocyte metabolism and fate specification, Journal of Immunology 196(1): 133.12.
- Xin B.T., Bruin G. de, Plomp J.W., Florea B.I., Marel G.A. van der & Overkleeft H.S. (2016), Incorporation of the Constrained Peptidomimetic, 5-Methylpyridin-2-one into Peptide Vinyl Sulfones and Peptide Epoxy Ketones is Detrimental for Proteasome Inhibition, European Journal of Organic Chemistry (6): 1132-1144.
- Riaz M., Raz Y., Putten M. van, Paniagua-Soriano G., Krom Y.D., Florea B.I. & Raz V. (2016), PABPN1-Dependent mRNA Processing Induces Muscle Wasting, PLOS GENETICS 12(5): .
- Bruin G. de, Xin B.T., Kraus M., Stelt M. van der, Marel G.A. van der, Kisselev A.F., Driessen C., Florea B.I. & Overkleeft H.S. (2016), A Set of Activity-Based Probes to Visualize Human (Immuno)proteasome Activities, Angewandte Chemie (International Edition) 55(13): 4199-4203.
- Ben Bdira F., Jiang J., Kallemeijn W.W., Haan A. de, Florea B.I., Bleijlevens B., Boot R.G., Overkleeft H.S., Aerts J.M.F.G. & Ubbink M. (2016), Hydrophobic interactions contribute to conformational stabilization of endoglycoceramidase II by mechanism-based probes, Biochemistry 55(34): 4823-4835.
- Stolze S.C., Liu N., Wijdeven R.H., Tuin A.W., Nieuwendijk A.M. van den, Florea B.I., Stelt M. van der, Marel G.A. van der, Neefjes J.J. & Overkleeft H.S. (2016), Photo-crosslinking of clinically relevant kinases using H89-derived photo-affinity probes, Molecular Biosystems 12(6): 1809-1817.
- Jiang J., Kuo C.L., Wu L., Franke C., Kallemeijn W.W., Florea B.I., Meel H.M.M. van, Marel G.A. van der, Codée J.D.C., Boot R.G., Davies G.J., Overkleeft H.S. & Aerts J.M.F.G. (2016), Detection of Active Mammalian GH31 α-Glucosidases in Health and Disease Using In-Class, Broad-Spectrum Activity-Based Probes, ACS Central Science 2(5): 351-358.
- Bruin G. de, Xin B.T., Florea B.I. & Overkleeft H.S. (2016), Proteasome Subunit Selective Activity-Based Probes Report on Proteasome Core Particle Composition in a Native Polyacrylamide Gel Electrophoresis Fluorescence-Resonance Energy Transfer Assay, Journal of the American Chemical Society 138(31): 9874-9880.
- Bruin G. de, Mock E.D., Hoogendoorn S., Nieuwendijk A.M.C.H. van den, Mazurek J., Marel G.A. van der, Florea B.I. & Overkleeft H.S. (2016), Enantioselective synthesis of adamantylalanine and carboranylalanine and their incorporation into the proteasome inhibitor bortezomib, Chemical Communications 52(21): 4064-4067.
- Banno A., Garcia D.A., Baarsel E.D. van, Metz P.J., Fisch K., Widjaja C.E., Kim S.H., Lopez J., Chang A.N., Geurink P.P., Florea B.I., Overkleeft H.S., Ovaa H., Bui J.D., Yang J. & Chang J.T. (2016), Downregulation of 26S proteasome catalytic activity promotes epithelial-mesenchymal transition, OncoTarget 7(16): 21527-21541.
- Xin B.T., Schimmack G., Du Y., Florea B.I., Marel G.A. van der, Driessen C., Krappmann D. & Overkleeft H.S. (2016), Development of new Malt1 inhibitors and probes, Bioorganic & Medicinal Chemistry 24(15): 3312-3329.
- Paniagua Soriano G., Besse L., Li N., Kraus M., Besse A., Meeuwenoord N., Bader J., Everts B., Dulk H. den, Overkleeft H.S., Florea B.I. & Driessen C. (2016), Proteasome inhibibor-adapted myeloma cells are largely independent from proteasome activity and show complex proteomic changes, in particular in redox and energy metabolism, Leukemia 30: 2198-2207.
- Xin B.T., Bruin G. de, Huber E.M., Besse A., Florea B.I., Filippov D.V., Marel G.A. van der, Kisselev A.F., Stelt M. van der, Driessen C., Groll M. & Overkleeft H.S. (2016), Structure-Based Design of β5c Selective Inhibitors of Human Constitutive Proteasomes, Journal of Medicinal Chemistry 59(15): 7177-7187.
- Latorre A., Rodriguez S., Gonzalez F.V., Florea B.I. & Overkleeft H.S. (2015), Synthetic Studies on the Preparation of Alanyl Epoxysulfones as Cathepsin Cysteine Protease Electrophilic Traps, Journal of Organic Chemistry (JOC) 80(15): 7752-7756.
- Jiang J., Kallemeijn W.W., Wright D.W., Nieuwendijk A.M.C.H. van den, Coco Rohde V., Folch E.C., Elst H. van den, Florea B.I., Scheij S., Donker-Koopman W.E., Verhoek M., Li N., Schürmann M., Mink D., Boot R.G., Codée J.D.C., Marel G.A. van der, Davies G.J., Aerts J.M.F.G. & Overkleeft H.S. (2015), In vitro and in vivo comparative and competitive activity-based protein profiling of GH29 alpha-L-fucosidases, Chemical Science 6(5): 2782-2789.
- Baggelaar M.P., Chameau P.J.P., Kantae V., Hummel J., Hsu K.L., Janssen F., Wel T. van der, Soethoudt M., Deng H., Den Dulk H., Allara M., Florea B.I., Di Marzo V., Wadman W.J., Kruse C.G., Overkleeft H.S., Hankemeier T., Werkman T.R., Cravatt B.F. & Stelt M. van der (2015), Highly Selective, Reversible Inhibitor Identified by Comparative Chemoproteomics Modulates Diacylglycerol Lipase Activity in Neurons, Journal of the American Chemical Society 137(27): 8851-8857.
- Ouairy C.M.J., Ferraz M.J., Boot R.G., Baggelaar M.P., Stelt M. van der, Appelman M., Marel G.A. van der, Florea B.I., Aerts J.M.F.G. & Overkleeft H.S. (2015), Development of an acid ceramidase activity-based probe, Chemical Communications 51(28): 6161-6163.
- Hsu M.H., Guo C.L., Liou A.Y., Chang T.Y., Ng M.C., Florea B.I., Overkleeft H.S., Wu Y.L., Liao J.C. & Cheng P.L. (2015), Stage-Dependent Axon Transport of Proteasomes Contributes to Axon Development, Developmental Cell 35(4): 418-431.
- Downey S.L., Florea B.I., Overkleeft H.S. & Kisselev A.F. (2015), Use of proteasome inhibitors, Current protocols in immunology 109: 9.10.1-9.
- Wiemhoefer A., Stargardt A., Linden W.A. van der, Renner M.C., Kesteren R.E. van, Stap J., Raspe M.A., Tomkinson B., Kessels H.W., Ovaa H., Overkleeft H.S., Florea B. & Reits E.A. (2015), Tripeptidyl Peptidase II Mediates Levels of Nuclear Phosphorylated ERK1 and ERK2, Molecular and Cellular Proteomics 14(8): 2177-2193.
- Wieber S., Florea B., Wagner A., Overkleeft H., Hecker M. & Drews O. (2014), Towards cell-specific targeting of proteasomes in cardiovascular cells, Acta Physiologica 210: 194-194.
- Li K.-Y., Kallemeijn W.W., Jiang J., Walvoort M.T.C., Willems L.I., Beenakker T., Elst H. van den, Marel G.A. van der, Codée J.D.C., Aerts J.M.F.G., Florea B.I., Boot R.G., Witte M.D. & Overkleeft H.S. (2014), Rational Design of Activity-Based Retaining β-Exoglucosidase Probes. In: Waldmann H. & Janning P. (Eds.), Concepts and Case Studies in Chemical Biology: Wiley. 191-206.
- Thijssen R., Geest C.R., Rooij M.F. de, Liu N., Florea B.I., Weller K., Overkleeft H.S., Oers M.H. van, Spaargaren M., Kater A.P. & Eldering E. (2014), Overcoming resistance to the novel BH3-mimetic ABT-199 in a CLL lymph node model; the role of ABL and BTK, Haematologica 99: 42-42.
- Li Hao, Van der Linden Wouter A., Verdoes Martijn, Florea Bogdan I., McAllister Fiona E., Govindaswamy Kavitha, Elias Joshua E., Bhanot Purnima, Overkleeft Herman S. & Bogyo Matthew (2014), Assessing Subunit Dependency of the Plasmodium Proteasome Using Small Molecule Inhibitors and Active Site Probes, ACS Chemical Biology 9(8): 1869-1876.
- Willems L.I., Beenakker T.J.M., Murray B., Scheij S., Kallemeijn W.W., Boot R.G., Verhoek M., Donker-Koopman W.E., Guimaraes da Lomba Ferraz M.J., Rijssel E.R. van, Florea B.I., Codee J.D.C., Marel G.A. van der, Aerts J.M.F.G. & Overkleeft H.S. (2014), Potent and Selective Activity-Based Probes for GH27 Human Retaining alpha-Galactosidases, Journal of the American Chemical Society 136(33): 11622-11625.
- Soriano Guillem Paniagua, De Bruin Gerjan, Overkleeft Herman S. & Florea Bogdan I. (2014), Toward Understanding Induction of Oxidative Stress and Apoptosis by Proteasome Inhibitors, Antioxidants & Redox Signaling 21(17): 2419-2443.
- Geurink Paul P., Van der Linden Wouter A., Mirabella Anne C., Gallastegui Nerea, De Bruin Gerjan, Blom Annet E.M., Voges Mathias J., Mock Elliot D., Florea Bogdan I., Van der Marel Gijs A., Driessen Christoph, Van der Stelt Mario, Groll Michael, Overkleeft Herman S. & Kisselev Alexei F. (2013), Incorporation of Non-natural Amino Acids Improves Cell Permeability and Potency of Specific Inhibitors of Proteasome Trypsin-like Sites, Journal of Medicinal Chemistry 56(3): 1262-1275.
- Baggelaar M.P., Janssen F.J., Esbroeck A.C.M. van, Dulk J. den, Allara M., Hoogendoorn S., McGuire R., Florea B.I., Meeuwenoord N.J., Elst H. van den, Marel G.A. van der, Brouwer J., Marzo V. di, Overkleeft H.S. & Stelt M. van der (2013), Development of an Activity-Based Probe and In Silico Design Reveal Highly Selective Inhibitors for Diacylglycerol Lipase-alpha in Brain, Angewandte Chemie (International Edition) 52(46): 12081-12085.
- Li N., Kuo C.L., Paniagua G., Elst H. van der, Verdoes M., Willems L.I., Linden W.A. van der, Ruben M., Genderen E. van, Gubbens J., Wezel G.P. van, Overkleeft H.S. & Florea B.I. (2013), Relative quantification of proteasome activity by activity-based protein profiling and LC-MS/MS, Nature Physics 8(6): 1155-1168.
- Kallemeijn W.W., Li K.Y., Witte M.D., Marques A.R.A., Aten J., Scheij S., Jiang J., Willems L.I., Voorn-Brouwer T.M., Roomen C.P.A.A. van, Ottenhoff R., Boot R.G., Elst H. van den, Walvoort M.T.C., Florea B.I., Codee J.D.C., Marel G.A. van der, Aerts J.M.F.G. & Overkleeft H.S. (2012), Novel Activity-Based Probes for Broad-Spectrum Profiling of Retaining beta-Exoglucosidases In Situ and In Vivo, Angewandte Chemie (International Edition) 51(50): 12529-12533.
- Li N., Overkleeft H.S. & Florea B.I. (2012), Activity-based protein profiling: an enabling technology in chemical biology research, Current Opinion in Chemical Biology 16(1-2): .
- Van der Linden W.A., Li N., Hoogendoorn S., Ruben M., Verdoes M., Guo J., Boons G.J., Van der Marel G.A., Florea B.I. & Overkleeft H.S. (2012), Two-step bioorthogonal activity-based proteasome profiling using copper-free click reagents: A comparative study, Bioorganic & Medicinal Chemistry 20(2): .
- Van der Linden W.A., Willems L.I., Shabaneh T.B., Li N., Ruben M., Florea B.I., Van der Marel G.A., Kaiser M., Kisselev A.F. & Overkleeft H.S. (2012), Discovery of a potent and highly beta 1 specific proteasome inhibitor from a focused library of urea-containing peptide vinyl sulfones and peptide epoxyketones, ORGANIC & BIOMOLECULAR CHEMISTRY 10(1): .
- Willems L.I., Li N., Florea B.I., Ruben M., Van der Marel G.A. & Overkleeft H.S. (2012), Triple Bioorthogonal Ligation Strategy for Simultaneous Labeling of Multiple Enzymatic Activities, Angewandte Chemie - International Edition in English 51(18): .
- Lanz H.L., Florea B.I., Noteborn M.H. & Backendorf C. (2012), Development and application of an in vitro apoptin kinase assay, Analytical Biochemistry 421(1): .
- Vermeij W.P., Florea B.I., Isenia S., Alia A., Brouwer J. & Backendorf C.M.P. (2012), Proteomic Identification of in Vivo Interactors Reveals Novel Function of Skin Cornification Proteins, Journal of Proteome Research 11(6): 3068-3076.
- Li J., Girard G., Florea B.I., Geurink P.P., Li N., Marel G.A. van der, Overhand M., Overkleeft H.S. & Wezel G.P. van (2012), Identification and isolation of lantibiotics from culture: a bioorthogonal chemistry approach, Organic and Biomolecular Chemistry 10(43): 8677-8683.
- Gubbens J., Janus M., Florea B.I., Overkleeft H.S. & Wezel G.P. van (2012), Identification of glucose kinase dependent and independent pathways for carbon control of primary metabolism, development and antibiotic production in Streptomyces coelicolor by quantitative proteomics, Molecular Microbiology 86(6): 1490-1507.
- Chang J.T., Ciocca M.L., Kinjyo I., Palanivel V.R., McClurkin C.E., De Jong C.S., Mooney E.C., Kim J.S., Steinel N.C., Oliaro J., Yin C.C., Florea B.I., Overkleeft H.S., Berg L.J., Russell S.M., Koretzky G.A., Jordan M.S. & Reiner S.L. (2011), Asymmetric Proteasome Segregation as a Mechanism for Unequal Partitioning of the Transcription Factor T-bet during T Lymphocyte Division, Immunity 34(4): .
- Clerc J., Li N., Krahn D., Groll M., Bachmann A.S., Florea B.I., Overkleeft H.S. & Kaiser M. (2011), The natural product hybrid of Syringolin A and Glidobactin A synergizes proteasome inhibition potency with subsite selectivity, Chemical Communications 47(1): .
- Hoogendoorn S., Habets K.L., Passemard S., Kuiper J., Marel G.A. van der, Florea B.I. & Overkleeft H.S. (2011), Targeted pH-dependent fluorescent activity-based cathepsin probes, Chemical Communications 47(33): .
- Geurink P.P., Florea B.I. & Overkleeft H.S. (2011), Activity-Based Profiling of 2-Oxoglutarate Oxygenases, Chemistry & Biology 18(5): .
- Mirabella A.C., Pletnev A.A., Downey S.L., Florea B.I., Shabaneh T.B., Britton M., Verdoes M., Filippov D.V., Overkleeft H.S. & Kisselev A.F. (2011), Specific Cell-Permeable Inhibitor of Proteasome Trypsin-like Sites Selectively Sensitizes Myeloma Cells to Bortezomib and Carfilzomib, Chemistry & Biology 18(5): .
- Willems L.I., Van der Linden W.A., Li N., Li K.Y., Liu N., Hoogendoorn S., Van der Marel G.A., Florea B.I. & Overkleeft H.S. (2011), Bioorthogonal Chemistry: Applications in Activity-Based Protein Profiling, Accounts of Chemical Research 44(9): .
- Willems L.I., Verdoes M., Florea B.I., Van der Marel G.A. & Overkleeft H.S. (2010), Two-step labeling of endogenous enzymatic activities by Diels-Alder ligation, ChemBioChem 11(12): 1769–1781.
- Geurink P.P., Florea B.I., Van der Marel G.A., Kessler BM & Overkleeft H.S. (2010), Probing the proteasome cavity in three steps: bio-orthogonal photo-reactive suicide substrates, Chemical Communications (46): 9052-9054.
- Risseeuw M.D., Florea B.I., Van der Marel G.A., Overkleeft H.S. & Overhand M. (2010), Sugar amino acid based peptide epoxyketones as potential proteasome inhibitors, Bioorg Chem 38(5): 202-209.
- Screen M., Britton M., Downey S.L., Verdoes M., Voges M.J., Blom A.E., Guerink P.P., Risseeuw M.D., Florea B.I., Van der Linden W.A., Pletnev A.A., Overkleeft H.S. & Kisselev A.F. (2010), The nature of pharmacophore influences active site specificity of proteasome inhibitors, Journal of Biological Chemistry 285(51): 40125-40134.
- Geurink P.P., Florea B.I., Li N., Witte M.D., Verasdonck J., Kuo C.L., Van der Marel G.A. & Overkleeft H.S. (2010), A cleavable linker based on the levulinoyl ester for activity-based protein profiling, Angewandte Chemie - International Edition in English 49(38): .
- Witte M.D., Kallemeijn W.W., Aten J., Li K.Y., Strijland A., Donker-Koopman W.E., Nieuwendijk A.M.C.H. van den, Bleijlevens B., Kramer G., Florea B.I., Hooibrink B., Hollak C.E.M., Ottenhoff R., Boot R.G., Marel G.A. van der, Overkleeft H.S. & Aerts J.M.F.G. (2010), Ultrasensitive in situ visualization of active glucocerebrosidase molecules, Nature Chemical Biology 6(12): 907-913.
- Geurink P.P., Liu N., Spaans M.P., Downey S.L., Nieuwendijk A.M.C.H. van der, Marel G.A. van der, Kisselev A.F., Florea B.I. & Overkleeft H.S. (2010), Incorporation of Fluorinated Phenylalanine Generates Highly Specific Inhibitor of Proteasome's Chymotrypsin-like Sites, Journal of Medicinal Chemistry 53(5): 2319-2323.
- Verdoes M., Willems L.I., Van der Linden W.A., Duivenvoorden B.A., Van der Marel G.A., Florea B.I., Kisselev A.F. & Overkleeft H.S. (2010), A panel of subunit-selective activity-based proteasome probes, Organic and Biomolecular Chemistry 8(12): .
- Clerc J., Li N., Krahn D., Groll M., Bachmann A.S., Florea B.I., Overkleeft H.S. & Kaiser M. (2010), The natural product hybrid of Syringolin A and Glidobactin A synergizes proteasome inhibition potency with substrate selectivity, Chemical Communications : .
- Florea B.I., Verdoes M., Li N., Van der Linden W.A., Geurink P.P., Van den Elst H., Hofmann T., De Ru A., Van Veelen P.A., Tanaka K., Sasaki K., Murata S., Den Dulk H., Brouwer J., Ossendorp F.A., Kisselev A.F. & Overkleeft H.S. (2010), Activity-based profiling reveals reactivity of the murine thymoproteasome-specific subunit beta5t, Chemistry & Biology 17(8): .
- Van der Linden W.A., Geurink P.P., Oskam C., Van der Marel G.A., Florea B.I. & Overkleeft H.S. (2010), Proteasome selectivity towards Michael acceptor containing oligopeptide-based inhibitors, Organic and Biomolecular Chemistry 8(8): .
- Semrau S., Monster M.W.L., Knaap M. van der, Florea B.I., Schmidt T. & Overhand M. (2010), Membrane lysis by gramicidin S visualized in red blood cells and giant vesicles, Biochimica et Biophysica Acta - Biomembranes 1798(11): .
- Verdoes M., Florea B.I., Hillaert U., Willems L.M., Linden W.A. van der, Sae-Heng M., Filippov D.V., Kisselev A.F., Marel G.A. van der & Overkleeft H.S. (2008), Azido-BODIPY acid reveals quantitative Staudinger-Bertozzi ligation in two-step activity based proteasome profiling, ChemBioChem 9: 1735-1738.
- Verdoes M. & Florea B.I. (2007), Mixing of peptides and electrophilic traps gives rise to potent, broad-spectrum proteasome inhibitors, ORGANIC & BIOMOLECULAR CHEMISTRY 5(9): 1416-1426.
- Verdoes M., Florea B.I., Linden W.A. van der, Renou D., Nieuwendijk A.M.C.H. van den, Marel G.A. van der & Overkleeft H.S. (2007), Mixing of peptides and electrophilic traps gives rise to potent, broad-spectrum proteasome inhibitors, Organic and Biomolecular Chemistry 5(9): 1416-1426.
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