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Functional analysis of Dof transcription factors controlling heading date and PPDK gene expression in rice

Promotor: P.J.J. Hooykaas, Co-Promotores: P.B.F. Ouwerkerk, M. Wang

Yu Zhang
22 december 2015
Thesis in Leiden Repository

Chapter 1 presents a general introduction to the factors involved in crop domestication and the specific role of transcription factors in this process. Chapter 2 describes the functional analysis of a Dof-type transcription factor, named OsDof24, from rice.chapter 3 describes the role of OsDof25 in the regulation of OsC4PPDK. Interactions between OsDof25 and OsC4PPDK were confirmed in experiments involving EMSA, yeast one-hybrid analysis and transient expression in rice protoplasts. The regulatory role of OsDof25 in OsC4PPDK expression was confirmed using OsDof25 misexpression plants. The results suggested that OsDof25 is a trans-activator of the OsC4PPDK gene in rice. Chapter 4 describes the effects of OsDof24 and OsDof25 on the content of rice seeds storage proteins. First the interaction between both OsDof24 and OsDof25 with the GluB-1 promoter was investigated by a set of in vitro and in vivo experiments. Results showed that both OsDof24 and OsDof25 can affect seed storage protein content probably by activating the transcription of the GluB-1 promoter. Finally, in Chapter 5, a general discussion of the results from Chapters 2, 3 and 4 is presented.