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Anti-microbial and Anti-biofilm compounds From Indonesian Medicinal Plants

Promotor: C.A.M.J.J. van den Hondel, Co-promotor: Sandra de Weert

Sylvia U.T. Pratiwi
08 december 2015
Thesis in Leiden Repository

Microbial biofilms causing elevated resistance to both most anti-microbial drugs and the host defense systems, which often results in persistent and difficult-to-treat infections. The discovery of anti-infective agents which are active against planktonic and biofilm microorganisms are therefore urgently required to deal with these biofilm-mediated infections. Plants are a rich source of new molecules with pharmacological properties for the development of new drugs. Indonesia is one of the countries which has a very diverse flora and a rich tradition in the use of medicinal plants. Since several Indonesian medicinal plants contain anti-microbial compounds it was considered conceivable that they might also be a source of new anti-biofilm compounds. The research present in this thesis has been focused on the screening and identification of Indonesian medicinal plant extracts and essential oils for their anti-microbial and anti-biofilm activity towards P. aeruginosa PAO1, Staphylococcus aureus Cowan I, and Candida albicans ATCC 10231. Preparative thin layer chromatography along with GC-MS and 1H-NMR elucidation were used to isolate and identify the active compound, and the result revealed that massoia lactone, the active compound of massoia oil, displayed potent activity against microbial biofilms in vitro and therefore has potential therapeutic implication for biofilm-associated microbial infections.