Dissertation
Enzyme-substrate interactions in the processive enzyme xylanase
The primary goal of this research is to deepen our understanding of substrate-enzyme interactions in Glycoside hydrolases. For this purpose, Bacillus circulans xylanase (BcX) has been selected as a well-established model to examine the Michaelis complex and glycosyl-enzyme adduct.
- Author
- M. Saberi
- Date
- 14 November 2024
- Links
- Thesis in Leiden Repository
The characteristic cleft shape of the active site of this enzyme family, equipped with six subsites, and presence of a SBS on the surface, in conjunction with the polymeric nature of their substrates, leads to complex binding patterns. Through detailed analyses, including NMR monitored titrations and leveraging pNMR effects, in combination with crystallography, we seek to provide a thorough investigation of substrate binding within the BcX active site.