Stephan Hacker
Assistant Professor
- Name
- Dr. S.M. Hacker
- Telephone
- +31 71 527 4362
- s.m.hacker@lic.leidenuniv.nl
Stephan Hacker studies the target proteins of covalent inhibitors proteome-wide with resolution of the addressed binding pocket using mass spectrometry, focusing on applications of this technology to identify new druggable targets for antibiotics.
PhD candidates
News
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Stephan Hacker elected into the board of Assistant Professor Network Netherlands -
Antibiotics of the future: looking for a new way to kill bacteria -
Vidi grants for 12 researchers from Leiden University -
Leiden researchers join forces against tuberculosis -
Leiden University strengthens its focus on Chemical Biology and Medicinal Chemistry with seven new group leaders
Assistant Professor
- Science
- Leiden Institute of Chemistry
- LIC/Chemical Biology
- LIC/CB/Molecular Physiology
- Dienemann J.N., Chen S.Y., Hitzenberger M., Sievert M.L., Hacker S.M., Prigge S.T., Zacharias M., Groll M. & Sieber S.A. (2023), A chemical proteomic strategy reveals inhibitors of lipoate salvage in bacteria and parasites, Angewandte Chemie (International Edition) 62(31): e202304533.
- Munoz A.W., Meighen-Berger K.M., Hacker S.M., Feige M.J. & Sieber S.A. (2023), A chemical probe unravels the reactive proteome of health-associated catechols, Chemical Science 14(32): 8635-8643.
- Weerapana E. & Hacker S.M. (2023), Activity-based protein profiling - celebrating the groundbreaking contributions of Benjamin Cravatt, Israel Journal of Chemistry 63(3-4): e202300059.
- Svenningsen E.B., Ottosen R.N., Jorgensen K.H., Nisavic M., Larsen C.K., Hansen B.K., Wang Y., Lindorff-Larsen K., Torring T., Hacker S.M., Palmfeldt J. & Poulsen T.B. (2022), The covalent reactivity of functionalized 5-hydroxy-butyrolactams is the basis for targeting of fatty acid binding protein 5 (FABP5) by the neurotrophic agent MT-21, RSC Chemical Biology 3(10): 1216-1229.
- Demange P., Joly E., Marcoux J., Zanon P.R.A., Listunov D., Rullière P., Barthes C., Noirot C., Izquierdo J.B., Rozié A., Pradines K., Hee R., Brito M.V. de, Marcellin M., Serre R.F., Bouchez O., Burlet-Schiltz O., Oliveira M.C.F., Ballereau S., Bernardes-Génisson V., Maraval V., Calsou P., Hacker S.M., Génisson Y., Chauvin R. & Britton S. (2022), SDR enzymes oxidize specific lipidic alkynylcarbinols into cytotoxic protein-reactive species, eLife 11: e73913.
- Schmidt C., Zollo M., Bonsignore R., Casini A. & Hacker S.M. (2022), Competitive profiling of ligandable cysteines in Staphylococcus aureus with an organogold compound, Chemical Communications 58(36): 5526-5529.
- Hacker S.M. (2022), Reversible protein inhibitors kept on target, Nature 603(7902): 583-584.
- Hacker S.M. & Jessen-Trefzer C. (2022), Chemical biology in drug discovery, Biological Chemistry 403(4): 361-362.
- Weigert Muñoz A., Hoyer E., Schumacher K., Grognot M., Taute K.M., Hacker S.M., Sieber S.A. & Jung K. (2022), Eukaryotic catecholamine hormones influence the chemotactic control of Vibrio campbellii by binding to the coupling protein CheW, Proceedings of the National Academy of Sciences 119(10): e2118227119.
- Abbasov M.E. Kavanagh M.E., Ichu T.A., Lazear M.R., Tao Y.F., Crowley V.M., Ende C.W..A., Hacker S.M., Ho J., Dix M.M., Suciu R., Hayward M.M., Kiessling L.L. & Cravatt B.F. (2021), A proteome-wide atlas of lysine-reactive chemistry, Nature Chemistry 13(11): 1081-+.
- Sima S., Barkovits K., Marcus K., Schmauder L., Hacker S.M., Hellwig N., Morgner N. & Richter K. (2021), HSP-90/kinase complexes are stabilized by the large PPIase FKB-6, Scientific Reports 11.
- Allihn P.W.A., Hackl M.W., Ludwig C., Hacker S.M. & Sieber S.A. (2021), A tailored phosphoaspartate probe unravels CprR as a response regulator in Pseudomonas aeruginosa interkingdom signaling, Chemical Science 12: 4763-4770.
- Hubner I., Shapiro J.A., Hossmann J., Drechsel J., Hacker S.M., Rather P.N., Pieper D.H., Wuest W.M. & Sieber S.A. (2021), Broad spectrum antibiotic xanthocillin X effectively kills acinetobacter baumannii via dysregulation of heme biosynthesis, ACS Central Science 7(3): 488-498.
- Bach K., Beerkens B.L.H., Zanon P.R.A. & Hacker S.M. (2020), Light-Activatable, 2,5-Disubstituted Tetrazoles for the Proteome-wide Profiling of Aspartates and Glutamates in Living Bacteria, ACS CENTRAL SCI 6(4): 546-554.
- Lee K.M., Le P., Sieber S.A. & Hacker S.M. (2020), Degrasyn exhibits antibiotic activity against multi-resistant Staphylococcus aureus by modifying several essential cysteines, Chemical Communications 56: 2929-2932.
- Le P.L., Kunold E., Macsics R., Rox K., Jennings M.C., Ugur I., Reinecke M., Chaves-Moreno D., Hackl M.W., Fetzer C., Mandl F.A.M., Lehmann J., Korotkov V.S., Hacker S.M., Kuster B., Antes I., Pieper D.H., Rohde M., Wuest W.M., Medina E. & Sieber S.A. (2020), Repurposing human kinase inhibitors to create an antibiotic active against drug-resistant Staphylococcus aureus, persisters and biofilms, Nature Chemistry 12: 145-+.
- Zanon P.R.A., Lewald L. & Hacker S.M. (2020), Isotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome, ANGEW CHEM INT EDIT 59(7): 2829-2836.
- Gleissner C.M.L., Pyka C.L., Heydenreuter W., Gronauer T.F., Atzberger C., Korotkov V.S., Cheng W.T., Hacker S.M., Vollmar A.M., Braig S. & Sieber S.A. (2019), Neocarzilin A Is a Potent Inhibitor of Cancer Cell Motility Targeting VAT-1 Controlled Pathways, ACS CENTRAL SCI 5(7): 1170-1178.
- Cravatt B.F., Hacker S.M. & Backus K.M. (2018), Lysine reactive probes and uses thereof ( octrooi nr. US20180372751A1), (Scripps Research Institute). [patent].
- Hacker S.M., Backus K.M., Lazear M.R., Forli S., Correia B.E. & Cravatt B.F. (2017), Global profiling of lysine reactivity and ligandability in the human proteome, Nature Chemistry 9: 1181-1190.
- Gotz K.H., Hacker S.M., Mayer D., Durig J.N., Stenger S. & Marx A. (2017), Inhibitors of the Diadenosine Tetraphosphate Phosphorylase Rv2613c of Mycobacterium tuberculosis, ACS CHEM BIOL 12(10): 2682-2689.
- Lange S., Hacker S.M., Schmid P., Scheffner M. & Marx A. (2017), Small-Molecule Inhibitors of the Tumor Suppressor Fhit, CHEMBIOCHEM 18(17): 1707-1711.
- Ermert S., Marx A. & Hacker S.M. (2017), Phosphate-Modified Nucleotides for Monitoring Enzyme Activity, TOPICS CURR CHEM 375.
- Ermert S., Hacker S.M., Buntru A., Scheffner M., Hauck C.R. & Marx A. (2017), Different Enzymatic Processing of gamma-Phosphoramidate and gamma-Phosphoester-Modified ATP Analogues, CHEMBIOCHEM 18(4): 378-381.
- Hacker S.M., Buntz A., Zumbusch A. & Marx A. (2015), Direct Monitoring of Nucleotide Turnover in Human Cell Extracts and Cells by Fluorogenic ATP Analogs, ACS CHEM BIOL 10(11): 2544-2552.
- Hacker S.M., Welter M. & Marx A. (2015), Synthesis of γ‐Phosphate‐Labeled and Doubly Labeled Adenosine Triphosphate Analogs, Current Protocols in Nucleic Acid Chemistry 60.
- Hacker S.M., Mortensen F., Scheffner M. & Marx A. (2014), Selective Monitoring of the Enzymatic Activity of the Tumor Suppressor Fhit, ANGEW CHEM INT EDIT 53(38): 10247-10250.
- Hacker S.M., Mortensen F., Scheffner M. & Marx A. (2014), Selektive Beobachtung der enzymatischen Aktivität des Tumorsuppressors Fhit, Angewandte Chemie 126(38): 10413-10416.
- Acosta O.B.G., Hardt N., Hacker S.M., Strittmatter T., Schink B. & Marx A. (2014), Thiamine Pyrophosphate Stimulates Acetone Activation by Desulfococcus biacutus As Monitored by a Fluorogenic ATP Analogue, ACS CHEM BIOL 9: 1263-1266.
- Hacker S.M., Hintze C., Marx A. & Drescher M. (2014), Monitoring enzymatic ATP hydrolysis by EPR spectroscopy, Chemical Communications 50: 7262-7264.
- Hardt N., Hacker S.M. & Marx A. (2013), Synthesis and fluorescence characteristics of ATP-based FRET probes, ORG BIOMOL CHEM 11(48): 8298-8305.
- Hacker S.M., Pagliarini D., Tischer T., Hardt N., Schneider D., Mex M., Mayer T.U., Scheffner M. & Marx A. (2013), Fluorogenic ATP Analogues for Online Monitoring of ATP Consumption: Observing Ubiquitin Activation in Real Time, ANGEW CHEM INT EDIT 52(45): 11916-11919.
- Hacker S.M., Pagliarini D., Tischer T., Hardt N., Schneider D., Mex M., Mayer T.U., Scheffner M. & Marx A. (2013), Fluorogene ATP-Analoga zur Detektion von ATP-Verbrauch: Beobachtung der Aktivierung von Ubiquitin in Echtzeit, Angewandte Chemie 125(45): 12133-12137.
- Hacker S.M., Hardt N., Buntru A., Pagliarini D., Mockel M., Mayer T.U., Scheffner M., Hauck C.R. & Marx A. (2013), Fingerprinting differential active site constraints of ATPases, Chemical Science 4: 1588-1596.
- Hacker S.M., Mex M. & Marx A. (2012), Synthesis and Stability of Phosphate Modified ATP Analogues, Journal of Organic Chemistry 77(22): 10450-10454.
- Consultant Drug Discovery Activities