Gideon Kersten
Extraordinary professor of Vaccine development
- Name
- Prof.dr. G.F.A. Kersten
- Telephone
- +31 71 527 4570
- g.f.a.kersten@lacdr.leidenuniv.nl
Dr. Gideon Kersten is professor of Vaccine Delivery at the Division of BioTherapeutics.
More information about Gideon Kersten
PhD Candidates
Former PhD candidates
Biography
Gideon Kersten is a vaccinologist and department head at the Institute for Translational Vaccinology, InTraVacc, in Bilthoven, The Netherlands. He is responsible for the analytical development and vaccine formulation development at InTraVacc. He has a master in Biochemistry (University of Leiden) and holds a PhD in Pharmacy on subunit vaccines (University of Utrecht). His research interests are in the development of antigen delivery systems, adjuvants and needle free delivery systems for vaccines, systems vaccinology and stabilisation of vaccines. Needle free delivery comprises dermal and mucosal vaccine delivery. Stabilisation comprises lyophilisationand spraydrying. With regard to analytics his interest is focused on the characterisation of (protein) antigens with immunochemical and physico-chemical techniques and the development of in vitro alternatives for animal testing.
Extraordinary professor of Vaccine development
- Science
- Leiden Academic Centre for Drug Research
- LACDR/Drug Delivery Technology
- Maas L. van der, Danial M., Kersten G.F.A., Metz B. & Meiring H.D. (2022), Mass spectrometry-based quantification of the antigens in aluminum hydroxide-adjuvanted diphtheria-tetanus-acellular-pertussis combination vaccines, Vaccines 10(7): 1078.
- Kooijman S., Vrieling H., Verhagen L., Ridder J. de, Haan A. de, Riet E. van., Heck A.J.R., Kersten G.F.A., Pennings J.L.A., Metz B. & Meiring H.D. (2022), Aluminum hydroxide and aluminum phosphate adjuvants elicit a different innate immune response, Journal of Pharmaceutical Sciences 111(4): 982-990.
- Westdijk J., Kogelman A., Put R. van der, Eksteen Z., Suarez D., Kersten G.F.A., Metz B. & Danial M. (2022), Immunochemical and biophysical characterization of inactivated Sabin poliovirus products: insights into rapid quality assessment tools, Journal of Pharmaceutical Sciences 111(4): 1058-1069.
- Rösch A.R., Zölls S., Stadler D., Helbig C., Wuchner K., Kersten G.F.A., Hawe A., Jiskoot W. & Menzen T. (2022), Particles in biopharmaceutical formulations, part 2: an update on analytical techniques and applications for therapeutic proteins, viruses, vaccines and cells, Journal of Pharmaceutical Sciences 111(4): 933-950.
- Michiels T.J.M., Veen M.A. van, Meiring H.D., Jiskoot W., Kersten G.F.A. & Metz B. (2021), Common reference-based tandem mass tag multiplexing for the relative quantification of peptides: design and application to degradome analysis of diphtheria toxoid, Journal of the American Society for Mass Spectrometry 32(6): 1490-1497.
- Schoenmaker L., Witzigmann D., Kulkarni J.A., Verbeke R., Kersten G.F.A., Jiskoot W. & Crommelin D.J.A. (2021), mRNA-lipid nanoparticle COVID-19 vaccines: structure and stability, International Journal of Pharmaceutics 601: 120586.
- Michiels TJ.M., Tilstra W., Hamzink M.R.J., Ridder J.W. de, Danial M., Meiring H.D., Kersten G.F.A., Jiskoot W. & Metz B. (2020), Degradomics-based analysis of tetanus toxoids as a quality control assay, Vaccines 8(4): 712.
- Michiels T.J.M., Schöneich C., Hamzink M.R.J., Meiring H.D., Kersten G.F.A., Jiskoot W. & Metz B. (2020), Novel formaldehyde-induced modifications of lysine residue pairs in peptides and proteins: identification and relevance to vaccine development, Molecular Pharmaceutics 17(11): 4375-4385.
- Michiels T.J.M. Meiring H.D. Jiskoot W. Kersten G.F.A. Metz B. (2020), Formaldehyde treatment of proteins enhances proteolytic degradation by the endo-lysosomal protease cathepsin S, Scientific Reports 10(1): 11535.
- Leone M.L., Romeijn S.G., Du G., Dévédec S.E. Le, Vrieling H., O'Mahony C., Bouwstra J.A. & Kersten G.F.A. (2020), Diphtheria toxoid dissolving microneedle vaccination: Adjuvant screening and effect of repeated-fractional dose administration, International Journal of Pharmaceutics 580: 119182.
- Raeven R.H.M. Rockx-Brouwer D. Kanojia G. Maas L. van der Bindels T.H.E. Have R. ten Riet E. van Metz B. Kersten G.F.A. (2020), Intranasal immunization with outer membrane vesicle pertussis vaccine confers broad protection through mucosal IgA and Th17 responses, Scientific Reports 10(1): 7396.
- Leone M., Romeijn S., Slütter B., O'Mahony C., Kersten G. & Bouwstra J.A. (2020), Hyaluronan molecular weight: Effects on dissolution time of dissolving microneedles in the skin and on immunogenicity of antigen, European Journal of Pharmaceutical Sciences 146: 105269.
- Raeven R.H.M., Maas L. van der, Pennings J.L.A., Fuursted K., Jørgensen C.S., Riet E. van, Metz B., Kersten G.F.A. & Dalby T. (2019), Antibody Specificity Following a Recent Bordetella pertussis Infection in Adolescence Is Correlated With the Pertussis Vaccine Received in Childhood, Frontiers in Immunology 10: 1364.
- Metz B., Michiels T., Uittenbogaard J., Danial M., Tilstra W., Meiring H.D., Hennink W.E., Crommelin D.J.A., Kersten G.F.A. & Jiskoot W. (2020), Identification of Formaldehyde-Induced Modifications in Diphtheria Toxin, Journal of Pharmaceutical Sciences 109(1): 543-557.
- Vrieling H., Kooijman S., Ridder J.W. de, Thies-Weesie D.M.E., Soema P.C., Jiskoot W., Riet E. van, Heck A.J.R., Philipse A.P., Kersten G.F.A., Meiring H.D., Pennings J.L. & Metz B. (2020), Activation of Human Monocytes by Colloidal Aluminum Salts, Journal of Pharmaceutical Sciences 109(1): 750-760.
- Vrieling H., Espitia Ballestas M., Hamzink M., Willems G.J., Soema P., Jiskoot W., Kersten G.F.A. & Metz B. (2019), Stabilised aluminium phosphate nanoparticles used as vaccine adjuvant, Colloids and Surfaces B: Biointerfaces 181: 648-656.
- Leone M., Priester M.I., Romeijn S., Nejadnik M.R., Mönkäre J., O'Mahony C., Jiskoot W., Kersten G. & Bouwstra J.A. (2019), Hyaluronan-based dissolving microneedles with high antigen content for intradermal vaccination: Formulation, physicochemical characterization and immunogenicity assessment, European Journal of Pharmaceutics and Biopharmaceutics 134: 49-59.
- Raeven R.H.M., Riet E. van, Meiring H.D., Metz B. & Kersten G.F.A. (2019), Systems vaccinology and big data in the vaccine development chain, Immunology 156(1): 33-46.
- Kooijman S., Brummelman J., Els C.A.C.M. van, Marino F., Heck A.J.R., Riet E. van, Metz B., Kersten G.F.A., Pennings J.L.A. & Meiring H.D. (2018), Vaccine antigens modulate the innate response of monocytes to Al(OH)3, PLoS ONE 13(5): e0197885.
- Kanojia G., Have R. ten, Brugmans D., Soema P.C., Frijlink H.W., Amorij J.P. & Kersten G.F.A. (2018), The effect of formulation on spray dried Sabin inactivated polio vaccine, European Journal of Pharmaceutics and Biopharmaceutics 129: 21-29.
- Du G., Woythe L., Maaden K. van der, Leone M., Romeijn S.G., Kros A., Kersten G.F.A., Jiskoot W. & Bouwstra J.A. (2018), Coated and Hollow Microneedle-Mediated Intradermal Immunization in Mice with Diphtheria Toxoid Loaded Mesoporous Silica Nanoparticles, Pharmaceutical Research 35(10): 189.
- Kanojia G., Raeven R.H.M., Maas L. van der, Bindels T.H.E., Riet E. van, Metz B., Soema P.C., Have R. ten, Frijlink H.W., Amorij J.P. & Kersten G.F.A. (2018), Development of a thermostable spray dried outer membrane vesicle pertussis vaccine for pulmonary immunization, Journal of Controlled Release 286: 167-178.
- Du G., Leone M., Romeijn S.G., Kersten G.F.A., Jiskoot W. & Bouwstra J.A. (2018), Immunogenicity of diphtheria toxoid and poly(I:C) loaded cationic liposomes after hollow microneedle-mediated intradermal injection in mice, International Journal of Pharmacological Research 547(1-2): 250-257.
- Raeven R.H.M., Brummelman J., Pennings J., Maas L. van der, Helm K., Tilstra W., Ark A. van der, Sloots A., Ley P. van der, Eden W. van, Jiskoot W., Riet E. van, Els C.A. van, Kersten G.F.A., Han W.G. & Metz B. (2018), Molecular and cellular signatures underlying superior immunity against Bordetella pertussis upon pulmonary vaccination, Mucosal Immunology 11(3): 979-993.
- Kooijman S., Brummelman J., Els C.A.C.M. van, Marino F., Heck A.J.R., Mommen G.P.M., Metz B., Kersten G.F.A., Pennings J.L.A. & Meiring H.D. (2018), Novel identified aluminum hydroxide-induced pathways prove monocyte activation and pro-inflammatory preparedness, Journal of Proteomics 175: 144-155.
- Soema P.C., Rosendahl Huber S.K., Willems G.J., Jacobi R., Hendriks M., Soethout E., Jiskoot W., Jonge J. de, Beek J. van, Kersten G.F.A. & Amorij J.-P. (2018), Whole-Inactivated Influenza Virus Is a Potent Adjuvant for Influenza Peptides Containing CD8+ T Cell Epitopes, Frontiers in Immunology 9: 525.
- Leone M., Oorschot B.H. van, Nejadnik M.R., Bocchino A., Rosato M., Kersten G.F.A., O’Mahony C., Bouwstra J.A. & Maaden K. van der (2018), Universal applicator for digitally-controlled pressing force and impact velocity insertion of microneedles into skin, Pharmaceutics 10(4): 211-219.
- Kanojia G., Have R. ten, Soema P.C., Frijlink H., Amorij J.-P. & Kersten G.F.A. (2017), Developments in the formulation and delivery of spray dried vaccines, Human Vaccines and Immunotherapeutics 13(10): 2364-2378.
- Raeven R.H.M., Pennings J.L.A., Riet E. van, Kersten G.F.A. & Metz B. (2017), Meta-Analysis of Pulmonary Transcriptomes from Differently Primed Mice Identifies Molecular Signatures to Differentiate Immune Responses following Bordetella pertussis Challenge, Journal of Immunology Research 2017: 8512847.
- Schipper P., Maaden K. van der, Groeneveld V.G.D., Ruigrok M.J.R., Romeijn S.G., Uleman S., Oomens C., Kersten G.F.A., Jiskoot W. & Bouwstra J.A. (2017), Diphtheria toxoid and N-trimethyl chitosan layer-by-layer coated pH-sensitive microneedles induce potent immune responses upon dermal vaccination in mice, Journal of Controlled Release 262: 28-36.
- Westdijk J., Metz B., Spruit N., Tilstra W., Gun J. van der, Hendriksen C. & Kersten G.F.A. (2017), Antigenic fingerprinting of diphtheria toxoid adsorbed to aluminium phosphate, Biologicals 47: 69-75.
- Kraan H., Soema P., Amorij J.-P. & Kersten G.F.A. (2017), Intranasal and sublingual delivery of inactivated polio vaccine, Vaccine 35(20): 2647-2653.
- Leone M., Monkare J.T., Bouwstra J.A. & Kersten G.F.A. (2017), Dissolving microneedle patches for dermal vaccination, Pharmaceutical Research 34(11): 2223-2240.
- Raeven R.H.M., Brummelman J., Pennings J.L.A., Maas L. van der, Tilstra W., Helm K., Riet E. van, Jiskoot W., Els C.A.C.M. van, Han W.G.H., Kersten G.F.A. & Metz B. (2016), Bordetella pertussis outer membrane vesicle vaccine confers equal efficacy in mice with milder inflammatory responses compared to a whole-cell vaccine, Scientific Reports 6: 38240.
- Raeven R.H.M., Brummelman J., Maas L. van der, Tilstra W., Pennings J.L.A., Han W.G.H., Els C.A.C.M. van, Riet E. van, Kersten G.F.A. & Metz B. (2016), Immunological signatures after Bordetella Pertussis infection demonstrate importance of pulmonary innate immune cells, PLOS ONE 11(10): 0164027.
- Kanojia G., Have R. ten, Bakker A., Wagner K., Frijlink H.W., Kersten G.F.A. & Amorij J.P. (2016), The production of a stable infliximab powder: The evaluation of spray and freeze-drying for production, PLOS ONE 11(10): 0163109.
- Schipper P., Maaden K. van der, Romeijn S.G., Oomens C., Kersten G.F.A., Jiskoot W. & Bouwstra J.A. (2016), Determination of depth-dependent intradermal immunogenicity of adjuvanted inactivated polio vaccine delivered by microinjections via hollow microneedles, Pharmaceutical Research 33: 2269-2279.
- Kanojia G., Willems G.J., Frijlink H.W., Kersten G.F.A., Soema P.C. & Amorij J.P. (2016), A design of experiment approach to predict product and process parameters for a spray dried influenza vaccine, International Journal of Pharmaceutics 511(2): 1098-1111.
- Kraan H., Have R.Ten, Maas L. van der, Kersten G.F.A. & Amorij J.P. (2016), Incompatibility of lyophilized inactivated polio vaccine with liquid pentavalent whole-cell-pertussis-containing vaccine, Vaccine 34(38): 4572-4578.
- Kraan H., Stel W. van der, Kersten G.F.A. & Amorij J.P. (2016), Alternative administration routes and delivery technologies for polio vaccines, Expert Review of Vaccines 15(8): 1029-1040.
- Schipper P., Maaden K. van der, Romeijn S., Oomens C., Kersten G., Jiskoot W. & Bouwstra J. (2016), Repeated fractional intradermal dosing of an inactivated polio vaccine by a single hollow microneedle leads to superior immune responses, Journal of Controlled Release 242: 141-147.
- Have R. ten, Reubsaet K., Herpen P. van, Kersten G.F.A. & Amorij J.-P. (2016), Demonstrating functional equivalence of pilot and production scale freeze-drying of BCG, PLOS ONE 11(3): e0151239.
- Soema P.C., Riet E. van, Kersten G.F.A. & Amorij J.P. (2015), Development of cross-protective influenza a vaccines based on cellular responses, Frontiers in Immunology 2015(6): 237.
- Soema P.C., Kompier R., Amorij J.P. & Kersten G.F.A. (2015), Current and next generation influenza vaccines: Formulation and production strategies, European Journal of Pharmaceutics and Biopharmaceutics 2015(94): 251-263.
- Soema P.C., Willems G.J., Jiskoot W., Amorij J.P. & Kersten G.F. (2015), Predicting the influence of liposomal lipid composition on liposome size, zeta potential and liposome-induced dendritic cell maturation using a design of experiments approach, European Journal of Pharmaceutics and Biopharmaceutics 94: 427-35.
- Maaden K. van der, Sekerdag E., Schipper P., Kersten G.F.A., Jiskoot W. & Bouwstra J.A. (2015), Layer-by-Layer Assembly of Inactivated Poliovirus and N-Trimethyl Chitosan on pH-Sensitive Microneedles for Dermal Vaccination, Langmuir 31(31): 8654-60.
- Raeven R.H., Maas L. van der, Tilstra W., Uittenbogaard J.P., Bindels T.H., Kuipers B., Ark A. van der, Pennings J.L., Riet E. van., Jiskoot W., Kersten G.F. & Metz B. (2015), Immunoproteomic profiling of bordetella pertussis outer membrane vesicle vaccine reveals broad and balanced humoral immunogenicity, Journal of Proteome Research 14(7): 2929-2942.
- Soema P.C., Rosendahl H.S.K., Willems G.J., Jiskoot W., Kersten G.F.A. & Amorij J.-P. (2015), Influenza T-cell Epitope-Loaded Virosomes Adjuvanted with CpG as a Potential Influenza Vaccine, PHARMACEUTICAL RESEARCH 32(4): 1505-1515.
- Hirschberg H.J.H.B., Riet E., Oosterhoff D., Bouwstra J.A. & Kersten G.F.A. (2015), Animal models for cutaneous vaccine delivery, European Journal of Pharmaceutical Sciences 71: 112-122.
- Maaden K. van der, Luttge R., Vos P.J., Bouwstra J.A., Kersten G.F.A. & Ploemen I. (2015), Microneedle-based drug and vaccine delivery via nanoporous microneedle arrays, Drug Delivery and Translational Research 5: 397-406.
- Have R. ten, Westdijk J., Levels L.M., Koedam P., Haan A. de, Hamzink M.R., Metz B. & Kersten G.F.A. (2015), Trypsin diminishes the rat potency of polio serotype 3, Biologicals 43(6): 474-478.
- Kraan H., Herpen P. van, Kersten G.F.A. & Amorij J.-P. (2014), Development of thermostable lyophilized inactivated polio vaccine, Pharmaceutical Research 31(10): 2618-2629.
- Kraan Heleen, Vrieling Hilde, Czerkinsky Cecil, Jiskoot Wim, Kersten Gideon & Amorij Jean-Pierre (2014), Buccal and sublingual vaccine delivery, JOURNAL OF CONTROLLED RELEASE 190: 580-592.
- Raeven R.H.M., Brummelman J., Pennings J.L.A., Nijst O.E.M., Kuipers B., Blok L.E.R., Helm K., Riet E. van, Jiskoot W., Els C.A.C.M. van, Han W.G.H., Kersten G.F.A. & Metz B. (2014), Molecular signatures of the evolving immune response in mice following a Bordetella pertussis infection, PLoS ONE 9(8): e104548.
- Els C. van, Mjaaland S., Naess L., Sarkadi J., Gonczol E., Smith Korsholm K., Hansen J., Jonge J. de, Kersten G.F.A., Warner J., Semper A., Kruiswijk C. & Oftung F. (2014), Fast vaccine design and development based on correlates of protection (COPs) : influenza as a trendsetter, Human Vaccines and Immunotherapeutics 10(7): 1935-1948.
- Riet E. van, Ainai A., Suzuki T., Kersten G.F.A. & Hasegawa H. (2014), Combatting infectious diseases: nanotechnology as a platform for rational vaccine design, Advanced Drug Delivery Reviews 74: 28-34.
- Tonnis W.F., Amorij J-P, Vreeman M.A., Frijlink H.W., Kersten G.F. & Hinrichs W.L.J. (2014), Improved storage stability and immunogenicity of hepatitis B vaccine after spray-freeze drying in presence of sugars, 55: 36-45.
- Ploemen Ivo H.J., Hirschberg Hoang J.H.B., Kraan Heleen, Zeltner Adrian, Van Kuijk Sandra, Lankveld Danielle P.K., Royals Michael, Kersten Gideon F.A. & Amorij Jean-Pierre (2014), Minipigs as an Animal Model for Dermal Vaccine Delivery, COMPARATIVE MEDICINE 64(1): 50-54.
- Bernard M., Wichard T., Put R. van der, Spruit N., IJssel J. van den, Robert J., Hendriksen C. & Kersten G. (2013), Physicochemical and immunochemical assays for monitoring consistent production of tetanus toxoid, Biologicals 41(4): 231-237.
- Westdijk J., Koedam P., Barro M., Steil B.P., Collin N., Vedvick T.S., Bakker W.A.M., Ley P. van der & Kersten G.F.A. (2013), Antigen sparing with adjuvanted inactivated polio vaccine based on Sabin strains, Vaccine 31(9): 1298-1304.
- Amorij J.P., Kersten G.F.A., Saluja V., Tonnis W.F., Hinrichs W.L.J., Slutter B., Bal S.M., Bouwstra J.A., Huckriede A. & Jiskoot W. (2012), Towards tailored vaccine delivery: Needs, challenges and perspectives, JOURNAL OF CONTROLLED RELEASE 161(2): 363-376.
- Hirschberg H., Kuijk S. van, Loch J., Jiskoot W., Bouwstra J., Kersten G. & Amorij J.P. (2012), A combined approach of vesicle formulations and microneedle arrays for transcutaneous immunization against hepatitis B virus, European Journal of Pharmaceutical Sciences 46(1-2): 1-7.
- Verrichten van onderzoek aan en ontwikkeling van vaccins