Cell Observatory

PhD Course Microscopy

This is a yearly event where we move in one week from the physics of light through the composition of the microscope, fluorescence microscopy and end up with some image analysis All the lectures are backed by practicals to really put your obtained knowledge into practice

Topics

• Physics of light
  • Starting with how light is created and which properties of light play a role in microscopy
• Microscope components
• Kohler illumination
  • The second step in the course is to know which components are in the microscope and how to set them up optimally
• Phase contrast
• DIC
• Dark field
  • In the third lecture contrast enhancing techniques are discussed and how they work to improve your images, but also how to set it up properly within the microscope
• Histology and sample preparation
  • In the 4th lecture we move the focus to preparing our samples optimally and as simple as possible
• Fluorescence microscopy
• Confocal microscopy
• Advanced fluorescence microscopy
  • Moving from brightfield to fluorescence to show what fluorescence is capable off and how to apply it optimally
• Image analysis
• Automated image analysis
  • On the last day of the course we focus on what we can do with the obtained images to extract quantifiable data. In the morning we learn how to do this manually in FIJI, in the afternoon we move to automated measurements, and recording of macros
• To obtain a certificate of participation an online quiz has to be completed
  (this will only be accessible during the course)