Triple Bioorthogonal Ligation Strategy for Simultaneous Labeling of Multiple Enzymatic Activities
Three at the same time: a ligation strategy combining tetrazine–norbornene cycloaddition, Staudinger–Bertozzi ligation, and copper(I)-catalyzed click reaction was used to label the three catalytic activities of the proteasome simultaneously in a single experiment. The orthogonality of the three ligation reactions enables selective monitoring of multiple targets at the same time in complex biological samples.
- Willems L.I., Li N., Florea B.I., Ruben M., Marel G.A. van der, Overkleeft H.S.
- 21 December 2012
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