Publication

Triple Bioorthogonal Ligation Strategy for Simultaneous Labeling of Multiple Enzymatic Activities

Three at the same time: a ligation strategy combining tetrazine–norbornene cycloaddition, Staudinger–Bertozzi ligation, and copper(I)-catalyzed click reaction was used to label the three catalytic activities of the proteasome simultaneously in a single experiment. The orthogonality of the three ligation reactions enables selective monitoring of multiple targets at the same time in complex biological samples.

Author

Willems L.I., Li N., Florea B.I., Ruben M., Marel G.A. van der, Overkleeft H.S.

Date

21 December 2012

Links

DOI link