Dissertation
Advancing image-based localization of lipid-based nanomedicines for the exploration of targeted drug delivery
Microscopic insight on lipid-based nanomedicine in vivo remains limited to the perception of the knowledge that could be obtained: if we interpret only what we see, then we only believe to know. Although believing to know encapsulates an undefined amount of uncertainty in the exploration of lipid-based nanomedicine, it remains a fundamental requirement to draw hypotheses that could result in major scientific breakthroughs.
- Author
- W.L. van Os
- Date
- 19 November 2024
- Links
- Thesis in Leiden Repository
Knowing the distribution of methylene blue within the body allowed Ehrlich to anticipate on exploring its therapeutic potential for antineuralgic and antimalarial use. In this light, by unravelling the specific locations of lipid-based nanomedicine upon screening of lipid composition, one could design its targeted therapy. Recent work underscores this concept by demonstrating that the evaluation of LNP localization in zebrafish embryos resulted in rationally designed LNP-mRNA delivery to the hepatic reticuloendothelial system in mice. The aim of this thesis was to advance the image-based localization of lipid-based nanomedicine, which could aid in improved understanding of targeted drug delivery. The work in this thesis aims to provide fundamental knowledge on the truthfulness of the source and the location of lipid-based nanomedicine and its in- and ex vivo environment.