Human skin equivalent barrier optimization
The currently available in vitro generated human skin equivalents resemble the human skin in many aspects. However, some essential barrier characteristics do not fully mimic the native barrier. Consequently, the human skin equivalents cannot be used for screening of drugs for skin penetration.
This project aims to improve the barrier function of these models, by optimizing environmental factors during the generation of these human skin equivalents. These environmental factors are considered to play a major role in the formation of a competent barrier. In this project a broad spectrum of analyses will be used.