Universiteit Leiden

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Research project

Efficient gene-targeting during meiosis

Efficient gene-targeting during meiosis

Duration
2013  -   2017
Contact
Sylvia de Pater
Funding
Partnership STW RijkZwaan project 12428 Partnership STW RijkZwaan project 12428

Using CRISPR/Cas9, DSB are induced in target sequences of the cruciferin 3 gene (A) or the protoporphyrinogen oxidase gene (B) for site-specific mutagenesis. Plants expressing these nucleases can be transformed with homologous repair templates to obtain specific mutations via homologous recombination (gene-targeting).

 

  • Shen H, Strunks GD, Klemann BJPM, Hooykaas PJJ, de Pater S (2017) CRISPR/Cas9-induced double-strand break repair in Arabidopsis nonhomologous end-joining mutants. doi: 10.1534/g3.116.035204

Connection with other research

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