Using CRISPR/Cas9, DSB are induced in target sequences of the cruciferin 3 gene (A) or the protoporphyrinogen oxidase gene (B) for site-specific mutagenesis. Plants expressing these nucleases can be transformed with homologous repair templates to obtain specific mutations via homologous recombination (gene-targeting).

 

• Shen H, Strunks GD, Klemann BJPM, Hooykaas PJJ, de Pater S (2017) CRISPR/Cas9-induced double-strand break repair in Arabidopsis nonhomologous end-joining mutants. doi: 10.1534/g3.116.035204

• crispr/cas9
• development & disease
• gene-targeting
• homologous recombination
• host-microbe interactions
• meiosis
• non-homologous end-joining
• talen