Universiteit Leiden

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Dissertation

CRISPR Cas-induced targeted mutagenesis with Agrobacterium mediated protein delivery

The RNA guided endonuclease based on the CRISPR/Cas system of Streptococcus pyogenus is a potent new tool for genome engineering in plants.

Author
Schmitz, D.J.
Date
20 September 2018
Links
Thesis in Leiden Repository

The RNA guided endonuclease based on the CRISPR/Cas system of Streptococcus pyogenus is a potent new tool for genome engineering in plants. Delivery of this system is usually done by transforming plants (and other organisms) with Agrobacterium tumefaciens which transfers a T-DNA encoding the required genes through its Type IV Secretion System (T4SS). Instead, here we report the delivery of the Cas9 protein of the CRISPR/Cas system of Streptococcus pyogenus through the T4SS of Agrobacterium to Nicotiana benthamiana and Saccharomyces cerevisiae.

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