PhD defence
Combinatorial testing of viral vector and CRISPR systems for precision genome editing
- Z. Li
- Date
- Wednesday 8 October 2025
- Time
- Location
- Lokhorstkerk
Pieterskerkstraat 1
2311 SV Leiden
Supervisor(s)
Summary
The work present in this dissertation provides the evidence that delivery of well-established CRISPR/Cas9 gene editing machinery rationally allocated in different viral vectors can achieve plausibly ideal results of gene editing in the mammalian cells regarding efficiencies, accuracy and specificity. The use of the CRISPR/Cas9 system makes the gene editing convenient and customized and compared to other delivery options, the viral vectors are outperformed due to natural evolution coffering high compatibility to mammalian cells. However, it would still be a long road with all kinds of obstacles. Finally, we may test in one clinical trial the concept of a combination such as the one investigated in the thesis.
PhD dissertations
Approximately one week after the defence, PhD dissertations by Leiden PhD students are available digitally through the Leiden Repository, that offers free access to these PhD dissertations. Please note that in some cases a dissertation may be under embargo temporarily and access to its full-text version will only be granted later.
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