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Programme

Metabolomics as a tool to investigate natural products  

  • 10:00 – 11:00 Introduction to metabolomics (Robert Verpoorte) 
  • 11:00 – 12:00 Application of metabolomics to medicinal plant research (Alvaro Viljoen)
  • 13:00 – 14:00 Metabolomics in human health and disease (Martin Giera)
  • 14:00 – 15:00 Revisiting Traditional Chinese Medicine by Metabolomics (Mei Wang)
  • 15:00 – 16:00 Metabolomics in Industry (Doris Jacobs)

How to extract and basic of data processing and multivariate data analysis

  • 10:00 – 11:00 Sample preparation (Hye Kyong Kim)
  • 11:00 – 12:30 Basic chromatography theory for metabolomics (Erica G. Wilson)
  • 14:00 – 15:00 Gas Chromatography-based metabolomics: theory and applications (Joachim Kopka)
  • 15:00 – 16:00 LC-MS Metabolomics: Theory and Applications (J. William Allwood)

How to detect metabolites by chromatography and spectroscopy

  • 10:00 – 11:30 Chromatography and spectroscopy data processing for metabolomics (Jeroen Jansen)
  • 11:30 – 13:00 Basic of multivariate data analysis (Harald van Mil)
  • 14:00 – 15:00 TLC application to medicinal plant metabolic fingerprinting (Tien Do)
  • 15:00 – 16:30 NMR based metabolomics: Theory and Applications (Jan Schripsema)

Metabolomics applications in life sciences

  • 10:00 – 11:00 Integrating metabolomic data from multiple analytical platforms for a comprehensive characterization of lemon essential oil(Serge Rudaz)
  • 11:00 – 12:30 Strategies for unambiguous identification of biomarkers (Jean-Luc Wolfender)
  • 14:00 – 15:00 NMR-based profiling of intra – and extracellular metabolome (Sarantos Kostidis)
  • 15:00 – 16:30 Metabolomics applications in agriculture and marine research (David Overy)

Techniques in detail

  • 10:00 – 11:00 Ambient mass spectrometry a useful tool for metabolomics (Teris van Beek)
  • 11:00 – 12:00 Identification of Metabolites by MS spectrometry (Rob van der Heijden)
  • 14:00 – 15:00 Application of in-vivo NMR for plant physiology (François Mesnard)

‘Sample Preparation and Extraction‘ and ‘LC-MS and NMR Measurements’

  • 11:00 – 12:00 Sample Preparation and Extraction (pre-record Video)
  • 12:00 – 12:30 Q & A for sample preparation and extraction
  • 14:00 – 15:00 LC-MS and NMR measurements (pre-record Video)
  • 15:00 – 16:00 Q & A for LC-MS and NMR measurements

Data processing of LC-MS

  • 11:00 – 12:00 LC-MS data processing (Ozlem Erol)
  • 12:00 – 12:30 Q & A for LC-MS data processing
  • 14:00 – 15:00 NMR data processing based on Mnova NMR suite (Young Hae Choi)
  • 15:00 – 15:30 Q & A for NMR data processing

Identification by LC-MS and multivariate data analysis

  • 11:00 – 12:00 MetaboScape (Bruker) application to LC-MS metabolomics (Rob van der Heijden)
  • 12:00 – 12:30 Q & A for the application of MetaboScape
  • 14:00 15:00 Multivariate data analysis (PCA and PLS) based on SIMCA (Young Hae Choi)
  • 15:00 – 15:30 Q & A for Multivariate data analysis

Discussion

  • 11:00 – 13:00 Discussion the homework of multivariate data analysis (Young Hae Choi)

Structural elucidation of amino acids I

  • 10:00 – 12:00 Structural elucidation of amino acids I-1 (Young Hae Choi)
  • 14:00 – 16:00 Structural elucidation of amino acids I-2 (Young Hae Choi)

Structural elucidation of amino acids II

  • 10:00 – 12:00 Structural elucidation of amino acids II-1 (Young Hae Choi)
  • 14:00 – 16:00 Structural elucidation of amino acids II-2 (Young Hae Choi)

Structural elucidation of amino acids III

  • 10:00 – 12:00 Structural elucidation of amino acids III-1 (Young Hae Choi)
  • 14:00 – 16:00 Structural elucidation of amino acids III-2 (Young Hae Choi)

Structural elucidation of sugars

  • 10:00 – 12:00 Structural elucidation of sugars (Young Hae Choi)
  • 14:00 – 16:00 Structural elucidation of sugars (Young Hae Choi)
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