Research project
Protein tyrosine kinases and cancer progression
We use a combination of different approaches to unravel the role of protein tyrosine kinases in the development of breast cancer and the onset of metastasis.
- Contact
- Bob van de Water
Our main interest is on focal adhesion kinase (FAK), Src kinase, epidermal growth factor receptor (EGFR) and their downstream targets. The role of these proteins in mammary gland development and the early steps in mammary tumorigenesis is determined by three-dimensional cell culture and MEC transplantation and experimental metastasis models. Their role in metastasis is determined via migration and invasion assays as well as in vivo lung metastasis models. To identify novel drug targets we use different high-throughput microscopy-based RNA interference screens. Such screens are combined with different read-outs, such as cell migration, focal adhesion turnover, and Fluorescence Resonance Energy Transfer (FRET) imaging.
Related research
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Protein tyrosine kinases and cancer progression
- Oncogenic protein tyrosine kinases and Annexin family members in breast cancer formation and metastasis
- Validation of relevant in vitro and in vivo models for evaluating efficacy of estrogen receptor antagonists
- A functional screen to identify kinases that affect ER-α responsiveness to tamoxifen
- The role of focal adhesion kinase in mammary gland development and mammary tumorigenesis
- Focal adhesion kinase (FAK)-mediated signaling in breast cancer cell migration and metastasis