Promotor: Prof.dr. B. van de Water, Co-promotores: L.T.M. van der Ven, A.S. Kienhuis

Single-Molecule Microscopy (SMM) techniques constitute a group of powerful imaging tools that enable researchers to study the dynamic behavior of individual molecules.

Death in all types of melanomas is generally caused by metastasis. Uveal melanoma (UM) is the most common intraocular melanoma, there are currently no (patient-derived) animal models that faithfully recapitulate metastatic dissemination of UM.

One of the major limitations in culturing complex tissues or organs is the lack of vascularization in the cultured tissue. Development of a functional capillary bed could overcome this problem.

Lysosomal storage disorders (LSDs) are a group of orphan diseases characterized by lysosomal dysfunction or impaired lysosomal catabolism and affect collectively about 1 in 5000 live births.

Promotor: Prof.dr. H.P. Spaink, Co-promotor: Dr. B.E. Snaar

Promotor: Prof.dr. M.K. Richardson

Promotores: Prof.dr. P.C.W. Hogendoorn & Prof.dr. H.P. Spaink Co-promotor: Dr. B.E. Snaar-Jagalska

Promotor: A.H. Meijer, Co-promotor: B.E. Snaar-Jagalska

New drugs for use as tuberculosis (TB) treatment are needed due to the constrains of classical antibiotics against TB and the rise of antibiotic-resistant strains, making TB a harder and harder disease to treat.

In this thesis, we have studied the potential of the zebrafish larval model in studying the ECS, as a complementary model to the existing rodent models.

In this thesis, we will utilize embryonic zebrafish tumour models to understand the interaction between engrafted human cancer cells and macrophages from the host, test drug administration modalities and anti-cancer efficacies of newly-developed PDT and PACT compounds, and test a light-triggered liposomal…

Promotor: Prof.dr. J.N. Kok

The research described in this thesis has, using the zebrafish as a model system, shed new light on the intricate relationship between TB and DM2, in particular on the role of leptin, SHP-1 and glucocorticoids.

This thesis focuses on the role of chemokine receptors CXCR3 and CCR2 in the inflammatory process and infection control using the zebrafish model.

Prostate cancer (PCa) is one of the most prevalent cancer in males.

Promotores: Prof.dr. W.J.G.M. Peijnenburg & Dr. M.G. Vijver

The main aim of my Ph.D. thesis is to further explore the value of using early developing zebrafish larvae (up to 5 dpf) as a model to study anxiety-like behaviour and their pharmacological modulation with drugs.

The work described in this dissertation contributes to a better mechanistic understanding of nanoparticles in vivo. To achieve that goal, we used the zebrafish as a highly predictive pre-screening model of nanoparticles.

The zebrafish is a promising vertebrate model organism in early drug discovery and development.

Promotor: Carel J. ten Cate, Co-Promotor: Hans W. Slabbekoorn

Promotor: Prof.dr. H.P. Spaink, Co-promotor: Prof.dr. A.H. Meijer

Promotor: Prof.dr. J. den Hertog

Promotor: H.P. Spaink, A.H. Meijer Co-promotor: R. Marin-Juez

Niet alleen evolutie stuurt verandering in soorten, het bouwplan van embryo’s bepaalt welke variaties echt ontstaan. Dat tonen Leidse biologen aan in een Nature-publicatie over de evolutionaire ontwikkeling van reptielpoten en vogelvleugels.

This thesis focuses on two processes involved in fighting infections: metabolism and immune cell motility and navigation.

We strive to address the challenge of constructing a modeling language to write software which can take advantage of recent hardware developments (multicore, cloud) without compromising in its abstraction levels.

Promotor: Roeland M.H. Merks

Description of Biotic ligand models: making an inventory of available models & assessment of the variability of the predicted HC5 using the BLMs in different Dutch water types (2005 - 2007)

In this thesis, I study 1) metabolic alterations in tuberculosis related to wasting syndrome in human patients as well as in rodent and fish animal models. 2) effects of the mutation of the leptin gene on cachexia and diabetes in rodent and zebrafish animal models.

The effective treatment of tuberculosis (TB) remains a major challenge to global health.

... which builds on self-regulation and stress-regulation theories and considers the modification of cognitions as a mediator of therapeutic change.

In this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms.

Epithelial-mesenchymal plasticity (EMP) and tumor cell migration play an important role in cancer progression, and an improved understanding of the mechanisms underlying these concepts is essential for developing new targeted approaches.

Real-life processes are characterized by dynamics involving time. Examples are walking, sleeping, disease progress in medical treatment, and events in a workflow.

Promotor: Prof.dr. E. Deprettere

Kunst en vrije tijd, Kunst en vrije tijd

This dissertation studies the Asymptotics of Bayesian nonparametric inference for Gaussian linear models.

Leidse moleculair-celbiologen in de onderzoeksgroep van Annemarie Meijer hebben nieuwe genen ontdekt die specifiek tot expressie komen tijdens de vroege ontwikkeling van macrofagen in de zebravis, waaronder een signaaleiwit dat essentieel is voor de migratie van macrofagen tijdens de immuunreactie op…

Industrial manufacturing processes, such as the production of steel or the stamping of car body parts, are complex semi-batch processes with many process steps, machine parameters and quality indicators.

The function of TLRs in innate immunity has aroused worldwide attention soon after its discovery. Because of the broad functions of TLR2 in innate immunity, the drive for the development of TLR2-targeted vaccines or therapeutic treatments has accelerated in the last decades.

Immunotherapies for cancer are an emerging class of therapeutic strategies which aim to treat cancer via augmentation of the immune system.

Control of infectious diseases poses continuous challenges for human health.

Making a dynamic substance stock model in order to conduct scenario analyses and make forecasts.

Promotor: Prof.dr. J.N. Kok, Co-promotor: F.J. Verbeek

The molecular mechanisms that instigate a healthy cell to become malignant are fueled by (epi)genetic alterations in so-called driver genes.

This dissertation focuses on developing new mathematical and statistical methods to properly represent time-varying covariates and model them within the context of time-to-event analysis.

From surface science towards industrial practice using high-pressure scanning tunneling microscopy.

Peter Grunwald

In this thesis, we extend the concept of null models as canonical ensembles of multi-graphs with given constraints and present new metrics able to characterize real-world layered systems based on their correlation patterns.