685 zoekresultaten voor “single cell transcriptomics” in de Publieke website
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Genetic-Tracing of CD8+ T cell fate decisions
Promotie
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Gene regulation in embryonic development
The human body consists of hundreds, perhaps thousands of different types of cells, each with different morphologies and functions, despite having the same genome.
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Emerging approaches to study cell-cell interactions
The aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds.
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Visual analytics for spatially-resolved omics data at single cell resolution: Methods and Applications
The deeper understanding of an organism's pathology is important for developing treatments. Over centuries of systematic research, clinical researchers have demonstrated that the more information they acquire about the cellular properties and their organisation in the tissue, the better they can understand…
- Cell Observatory
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Novel Immune Cell-Based Therapies for Atherosclerosis
Promotor: Prof.dr. J. Kuiper
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PhD candidate in Bioinformatics Safety Assessment of Gene and Cell Therapy
Wiskunde en Natuurwetenschappen, Leiden Academic Centre for Drug Research (LACDR)
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Postdoc in the fields of single cell biophysics and biomolecular condensate mechanics
Wiskunde en Natuurwetenschappen, Leiden Academic Centre for Drug Research (LACDR)
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Single-molecule microscopy in zebrafish embryos
Single-Molecule Microscopy (SMM) techniques constitute a group of powerful imaging tools that enable researchers to study the dynamic behavior of individual molecules.
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Anisotropy in cell mechanics
Mechanical interactions between cells and their environment play an important role in many biological processes.
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Towards Optical Detection of a Single Electron
Single-molecule spectroscopy has become a powerful method for using organic fluorescent molecules in numerous applications.
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Two-photon multifocal microscopy for in vivo single-molecule and single-particle imaging
In this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms.
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B cell modulation in atherosclerosis
Cardiovascular disease is a major global burden and atherosclerosis is the main underlying pathological process.
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PhD in Quantitative Cell and Developmental Biology
Wiskunde en Natuurwetenschappen, Instituut Biologie Leiden (IBL)
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Chromatin dynamics in single genes
Hoe beinvloed de structuur van één enkel gen de activiteit van genen die gevoelig zijn voor hormonen?
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enhancement of one-photon- and two-photon-excited single-molecule fluorescence by single gold nanorods
This thesis is a collection of experimental attempts to enhance photoluminescence of fluorescent molecules and quantum dots with single gold nanorods (GNRs) and relevant applications.
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Nucleosome stacking in chromatin fibers probed with single-molecule force- and torque-spectroscopy
In human cells, a meter-long DNA is condensed inside a micrometer-sized cell nucleus. Simultaneously, the genetic code must remain accessible for its replication and transcription to functional proteins.
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Research Technician – tumor cell biology
Wiskunde en Natuurwetenschappen, Leiden Academic Centre for Drug Research (LACDR)
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Assistant/Associate Professor Biochemistry of the Bacterial Cell Wall and Cell Membrane (1.0 FTE)
Wiskunde en Natuurwetenschappen, Instituut Biologie Leiden (IBL)
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Quantitative live cell imaging of glucocorticoid receptor dynamics in the nucleus
In this thesis, the focus lies on studying glucocorticoid receptor dynamics in living cells with the aim of understanding how this transcription factor finds its DNA target sites to regulate transcription.
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Computational modeling of cellular dynamics in tumor cell migration
Epithelial-mesenchymal plasticity (EMP) and tumor cell migration play an important role in cancer progression, and an improved understanding of the mechanisms underlying these concepts is essential for developing new targeted approaches.
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Photothermal circular dichroism studies of single nanoparticles
In this work, we investigate the minute circular dichroism effects of single nanoparticles.To this aim, we apply photothermal imaging with a polarization-modulated heating beam.
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The physics of nanowire superconducting single-photon detectors
Promotores: Prof.dr. D. Bouwmeester, Prof.dr. A. Fiore (TU Eindhoven)
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Squaramide-based supramolecular materials for 3D cell culture applications
A new type of tripodal squaramide-based supramolecular hydrogels is developed and studied.
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Phenotypic screening with 3D cell-based assays
Traditional drug discovery approaches have been hampered by (in vitro) cell-culture models that poorly represent the situation in the human body.
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Multiscale mathematical biology of cell-extracellular matrix interactions during morphogenesis
During embryonic growth, cells proliferate, differentiate, and collectively migrate to form different tissues at the right position and time in the body.
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Off the wall: characterisation and exploitation of a cell wall deficient life style in filamentous actinomycetes
Almost all bacteria are enveloped by a cell wall that provides cellular protection.
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Microfluidic 3D cell culture for high throughput screening
There is an urgent need for more physiologically relevant cell culture methods to guide compound selection in pre-clinical stages of the drug development pipeline.
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Chitin in the fungal cell wall: towards valorization of spent biomass of Aspergillus niger
Aspergillus niger is an important industrial producer of organic acids and enzymes producing large amounts of spent fungal biomass.
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Mast cell-mediated immune modulation in experimental Rheumatoid Arthritis and Atherosclerosis
In this research project, we aimed to obtain more insight in the role of mast cells in the immune driven disorders rheumatoid arthritis and atherosclerosis.
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Another Brick in the Wall: The role of the actinobacterial cell wall in antibiotic resistance, phylogeny and development
Streptomyces are multicellular, Gram-positive bacteria in the phylum of actinobacteria which produce a high amount of bioactive natural products of which the expression is tightly coordinated with the life cycle.
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Insights from modeling metabolism and amoeboid cell motility in the immune system
This thesis focuses on two processes involved in fighting infections: metabolism and immune cell motility and navigation.
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Chemical Synthesis of Fragments of Streptococcal Cell Wall Polysaccharides
This thesis describes the design and synthesis of fragments of various cell wall carbohydrates of the Streptococcus species, including the branched Group B-specific antigen (GBC) of Group B Streptococcus, glycerol phosphate (GroP) modified group A carbohydrate (GAC), and the O-acetylated type 1 capsular…
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Structural changes in single chromatin fibers induced by tension and torsion
Promotor: T. Schmidt, Co-promotor: S. J.T. van Noort
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Exploring structure dependencies of gas-surface interactions with curved single crystals
Curved single crystals provide variable, but well-defined surface structures.
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Single-electrolyte isotachophoresis: on-chip analyte focusing and separation
Promotor: Thomas Hankemeier, Co-promotores: Heiko van der Linden, Paul Vulto
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Stable single molecules for quantum optics and all-optical switches
Promotor: Prof.dr. M.A.G.J. Orrit
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Postdoc candidate in biochemistry / cell glycobiology / protein targeted degradation
Wiskunde en Natuurwetenschappen, Leids Instituut voor Chemisch Onderzoek (LIC)
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Unraveling the mechanism of multicopper oxidases: from ensemble to single molecule
Promotores: Prof.dr. G.W. Canters, Prof.dr. T.J. Aartsma
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Forces and symmetries in cells and tissues
The way organisms develop from the initial single-cellular state to a complex final assembly like the human body, and how the final body is maintained throughout life, is one of the greatest mysteries and it’s understanding one of the biggest scientific challenges.
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Macrophages inhibit human osteosarcoma cell growth after activation with the bacterial cell wall derivative liposomal muramyl tripeptide in combination
CONCLUSION: This study demonstrates that human macrophages can be induced to exert direct anti-tumor activity against osteosarcoma cells. Our observation that the induction of macrophage anti-tumor activity by L-MTP-PE required IFN-gamma may be of relevance for the optimization of L-MTP-PE therapy in…
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Cell-autonomous and host-dependent CXCR4 signaling in cancer metastasis: insights from a zebrafish xenograft model
Promotor: A.H. Meijer, Co-promotor: B.E. Snaar-Jagalska
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RNA splicing in breast cancer progression
In this thesis, we aimed to better understand the underlying mechanisms involved in TNBC progression and metastasis formation and discover new targets to reduce breast cancer related deaths.
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The early stress response of jasmonic acid in cell suspension cultures of Catharanthus roseus
In order to gain a better insight into the basis of the rapid jasmonic acid (JA)-mediated stress response, an integrated approach using a targeted high-resolution mass spectrometry-based (HRMS) platform, was conducted to monitor the metabolism of JA using the model system of cell suspension cultures…
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Plasmonic enhancement of single-molecule fluorescence under one- and two-photon excitation
This thesis aims to improve the detection from ultra-weak single emitter by enhancing their emission properties with plasmonic nanostructures. We exploit the wet-chemically synthesized single crystalline gold nanorods (GNRs) as our basic frameworks in the whole studies, simply because of their unique…
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Structural characterization of the cell envelope of Actinobacteria under changing environments
Bacteria have the ability to alter their morphology in order to adapt to changing environments.
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Two-photon luminescence of gold nanorods: applications to single-particle tracking and spectroscopy
Gold nanorods are biocompatible nanoparticles that present an excellent two-photon signal that can be used to get high spatial resolution inside living cells. Gold nanorods are photostable and therefore can be followed inside cells for long time, with possible applications as trackers in live cells.
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Towards a single-molecule FRET study of Frauenfelder's nonexponential rebinding of CO in myoglobin
Early time-resolved experiments by Frauenfelder on the ensemble of the kinetic rebinding of CO to myoglobin molecules resulted in a stretched exponential relaxa-tion due to a very large spread of the reaction rates of individual molecules.
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Formulation of influenza T cell peptides: In search of a universal influenza vaccine
Promotores: Prof.dr. G.F.A. Kersten, W. Jiskoot, Co-promotor: J-P. Amorij
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hydrogels as synthetic extracellular matrices for three- dimensional cell culture
Synthetic hydrogels that mimic the natural extracellular matrix in the biophysical and biochemical cues it provides to cells are in high demand, however the cell phenotypes as they are observed in vivo in numerous cases have yet to be attained.