The aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds.

In het Cell Observatory zoomen we in op levende cellen. We bestuderen de processen van het leven op moleculair niveau en onderzoeken bijvoorbeeld hoe we de uitzaaiing van kanker tegen kunnen gaan en medicijnen kunnen ontwikkelen tegen ziekten zoals tuberculose. Medicijnontwikkeling is één van de kernactiviteiten…

Promotor: Prof.dr. J. Kuiper

Mechanical interactions between cells and their environment play an important role in many biological processes.

Single-molecule spectroscopy has become a powerful method for using organic fluorescent molecules in numerous applications.

In this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms.

Hoe beinvloed de structuur van één enkel gen de activiteit van genen die gevoelig zijn voor hormonen?

This thesis is a collection of experimental attempts to enhance photoluminescence of fluorescent molecules and quantum dots with single gold nanorods (GNRs) and relevant applications.

Cardiovascular disease is a major global burden and atherosclerosis is the main underlying pathological process.

Wiskunde en Natuurwetenschappen, Leiden Academic Centre for Drug Research (LACDR)

Promotores: Prof.dr. D. Bouwmeester, Prof.dr. A. Fiore (TU Eindhoven)

In this thesis, the focus lies on studying glucocorticoid receptor dynamics in living cells with the aim of understanding how this transcription factor finds its DNA target sites to regulate transcription.

Promotie

Promotie

A new type of tripodal squaramide-based supramolecular hydrogels is developed and studied.

Traditional drug discovery approaches have been hampered by (in vitro) cell-culture models that poorly represent the situation in the human body.

During embryonic growth, cells proliferate, differentiate, and collectively migrate to form different tissues at the right position and time in the body.

There is an urgent need for more physiologically relevant cell culture methods to guide compound selection in pre-clinical stages of the drug development pipeline.

Almost all bacteria are enveloped by a cell wall that provides cellular protection.

Aspergillus niger is an important industrial producer of organic acids and enzymes producing large amounts of spent fungal biomass.

In this research project, we aimed to obtain more insight in the role of mast cells in the immune driven disorders rheumatoid arthritis and atherosclerosis.

Promotor: T. Schmidt, Co-promotor: S. J.T. van Noort

Promotor: Thomas Hankemeier, Co-promotores: Heiko van der Linden, Paul Vulto

This thesis focuses on two processes involved in fighting infections: metabolism and immune cell motility and navigation.

Promotor: Prof.dr. M.A.G.J. Orrit

Streptomyces are multicellular, Gram-positive bacteria in the phylum of actinobacteria which produce a high amount of bioactive natural products of which the expression is tightly coordinated with the life cycle.

Promotores: Prof.dr. G.W. Canters, Prof.dr. T.J. Aartsma

In this thesis, we aimed to better understand the underlying mechanisms involved in TNBC progression and metastasis formation and discover new targets to reduce breast cancer related deaths.

Promotor: A.H. Meijer, Co-promotor: B.E. Snaar-Jagalska

Gold nanorods are biocompatible nanoparticles that present an excellent two-photon signal that can be used to get high spatial resolution inside living cells. Gold nanorods are photostable and therefore can be followed inside cells for long time, with possible applications as trackers in live cells.

In order to gain a better insight into the basis of the rapid jasmonic acid (JA)-mediated stress response, an integrated approach using a targeted high-resolution mass spectrometry-based (HRMS) platform, was conducted to monitor the metabolism of JA using the model system of cell suspension cultures…

Cytotoxic T-cells (CTLs) are involved in the clearance of viruses and killing of tumor cells.

Promotor: C.A.J. Knibbe, Co-promotor: J.J. Boelens, R.G.M. Bredius

Promotor: Prof.dr. R. Verpoorte, Co-Promotores: N.R. Mustafa, A.E. Schulte

Schneider

This thesis consists of two main parts. The first part (Chapters 2 and 3) deals with a class of Markov process called Quasi-Skipfree (QSF) processes.

Light-Harvesting Complex II (LHCII) is responsible for light absorption and excitation energy transfer in plants and photosynthetic algae, while in high light it undergoes conformational changes by which it quenches excitations to prevent photodamage.

The research described in this thesis focused on identifying novel drug targets and synergistic combinations for triple-negative breast cancer (TNBC), a virulent subtype of breast cancer with a dismal prognosis and limited therapeutic options.

Nierfalen is een serieus probleem omdat nieren zichzelf niet kunnen regenereren na ernstige beschadiging. Een mogelijke oplossing bestaat uit het kunstmatig laten groeien van gezond nierweefsel. Daarvoor moeten wetenschappers eerst de ontwikkeling begrijpen tijdens de allereerste fase, in de foetus.…

The focus throughout this thesis will be on gathering fundamental studies of the detailed structure and composition of the electrode/electrolyte interface effect on the rate and mechanism of key electrocatalytic reactions.

All life on earth contains DNA, which is used to store biological information. Organisms compact their DNA in order for it to fit inside their cell(s).

Shape and biological function are tightly connected. Physical descriptions are used to connect the shape of a biological system with its function.

Scattering of light in the presence of nano-structured materials, i.e. with features in the order of the wavelength of the light or smaller, reveals details of how light interacts with matter at the nanoscale.

Met de nodige maatregelen starten onderzoekers hun werkzaamheden in verschillende laboratoria weer deels op. Het Leidse Cell Observatory is één van de plekken waar wetenschappers sinds 11 mei hun labwerk hervatten.

Promotor: H.P. Spaink, A.H. Meijer Co-promotor: R. Marin-Juez

Embryonische stamcellen hebben het opmerkelijke vermogen om zich door te ontwikkelen tot elk type cel. Tijdens hun metamorfose richting bijvoorbeeld een lever- of hartcel, moeten ze steeds weer kiezen tussen verschillende ontwikkelingspaden. Hoe ze deze beslissingen maken is grotendeels onbekend. Een…

Promotor: T. Schmidt

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