Growth hormone profiles are pulsatile and highly variable between individuals, limiting the implementation of mathemathical models to quantify an individual's secretion.

Promotor: Prof.dr. H.S. Overkleeft

Less than 1 in 10 drug candidates that enter phase 1 clinical trials actually gets approved for human use.

In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

To this day, all cyclophellitol-based inhibitors and ABPs have been close analogues of their natural substrate counterparts. As a result, these probes showed high selectivity towards their target glycosidases.

Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.

Wiskunde en Natuurwetenschappen, Instituut Biologie Leiden (IBL)

Promotor: Prof.dr. T. Hankemeier

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-promotor: B.I. Florea

Wiskunde en Natuurwetenschappen, Leiden Institute of Advanced Computer Science (LIACS)

Wiskunde en Natuurwetenschappen, Instituut Biologie Leiden (IBL)

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

Overkleeft

Promotor: H.S. Overkleeft, Co-Promotor: B.I. Florea

Glycoside hydrolases (glycosidases/GHs) are widely abundant enzymes in all kingdoms of life and are important biocatalysts that catalyze the hydrolysis of glycosidic linkages in oligo/polysaccharides, glycoproteins and glycolipids with tremendous efficiency

Promotor: H.S. Overkleeft, J. Neefjes, Co-promotor: M. van der Stelt

The chemical synthesis of inhibitors and probes targeting endo-glycosidases.

Glycosidases are essential in fundamental biological processes and are responsible for the degradation of most (oligo)saccharides, glycolipids and glycoproteins.

Vitamin A or retinol is essential in embryonic development, the visual cycle and the immune system.

This thesis aimed to investigate the impact of exposure dynamics, relative contributions of ENPs(particle) and ENPs(ion), and dosing regimens on the toxicity of ENPs varying in different physico-chemical properties, on the composition and functioning of soil microbial communities.

1. To what extent do metallic NPs added to soil extractions change the activity, abundance, or community composition of microbes? 2. How do the effects of metallic NPs on soil microbes differ from the effects of the ions shedding from corresponding NPs?

CONSENT (Consumer sentiment regarding privacy on user generated content services in the digital economy) is a research project funded by the European Union under the 7th Framework Programme. The question central to this research is whether recent changes to consumer and commercial practices developed…

Promotie

This thesis describes the use of an activity-based proteomics method to study the endocannabinoid system.

The research profile ‘Asian Modernities and Traditions’ (AMT) aims to raise the strength and visibility of research, teaching and dissemination of Asian studies at Leiden University.

Promotie

Promotie

Promotie

Plants produce an astonishing variety of secondary metabolites (SMs) which are thought to play vital roles in the fitness of plants through ecological interactions.

Synthesis and application of chemical biology tools to study immunomodulatory signaling lipids.

Promotor: Prof.dr. B. van de Water

Promotie

Dark matter is one of the biggest mysteries of the Universe. Its properties cannot be explained with the known laws of physics and elementary particles.

Large parts of the research described in this Thesis aims at the development of oligopeptide-masked toxins and their in situ immunoproteasome-mediated activation.

Promotores: P.G.L. Klinkhamer, P.M. Brakefield, Co-Promotor: M. Bruinsma

Lysosomal glycosidases are acid hydrolases that fragment glycoconjugates in lysosomes.

Promotores: Prof.dr. C.A.M.J.J. van den Hondel, Prof.dr. V. Meyer (Technische Universität Berlin)

The major and ultimate aim of metabolomics is to obtain an answer to a specific biological or clinical question.

Promotor: Prof.dr. M. Schilthuizen

A novel mathematical model describes spatial-temporal drug distribution within one or more brain units, which are cubic representations of a piece of brain tissue with brain capillaries at the edges.

Promotie

Promotie

Promotie

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-Promotor: R.G. Boot

Promotores: Prof.dr. W.J.G.M. Peijnenburg & Dr. M.G. Vijver

The disease acute myeloid leukemia (AML) is characterized by fast progression and low survival rates.

Promotor: K.H. Kuijken