Promotor: Prof.dr. E.J.J. Groenen, Co-Promotor: M. Huber

Promotor: M. Ubbink, Co-promotor: G. Siegal

Promotor: Prof.dr. M. Ubbink, Co-promotor: G. Siegal

Promotor: Prof.dr. M. Ubbink

The thesis describes the use of electron paramagnetic resonance (EPR) spectroscopy, in continuous wave and pulse modes, to address the interaction of α-Synuclein (αS) with membranes and the aggregation of αS.

Protein–protein complexes are formed via transient states called encounter complexes that greatly influence the formation of the stereospecific complex.

The studies presented in the work show the potential of the integrative use of biophysical data in defining the structural basis of protein interactions.

The lipid membrane is a basic structural component of all living cells. Embedded in this nanometer-thin barrier, membrane proteins shape the membrane and at the same time respond to the shape of the membrane.

Het Protein Centre kan u voorzien van gezuiverde eiwitten die essentieel zijn voor vele experimenten, variërend van in-vitro-inhibitorscreening en enzymatische analyse tot manipulatie van levende cellen.

This ecological community has long been known to affect host biology, and their diverse roles have been further clarified in recent years following numerous studies of animal:microbiota interactions in diverse systems.

In this thesis I describe the results of Pulsed Interleaved Excitation and Fluorescence (Cross) Correlation Spectroscopy (PIE-F(C)CS) combined with single-pair Förster Resonance Energy Transfer (spFRET) used to study dynamics in single nucleosomes, which depends on subtle differences in the length of…

This thesis describes the use of bioorthogonal proteins in immunological settings. It provides an introduction towards the field of protein modification, which was used throughout the thesis for the expression of proteins containing unnatural amino acids.

Promotor: Prof.dr. M. Ubbink

Promotores: C.A.M.J.J. van den Hondel, V. Meyer, Co-promotor: A.F.J. Ram

In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.

The present thesis focuses on the pharmacological concept of drug-target interaction, which dates back to the beginning of modern pharmacology.

Promotor: Prof.dr. M. Ubbink

Promotor: W. Jiskoot, Co-promotor: A. Hawe

Mycoheterotrophy is a particular mode of life in which plants obtain carbohydrates from their associated fungal partners, instead of by using photosynthesis.

Supervisor: P.J.J. Hooykaas Co-Supervisor: R. Offringa

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

In our research protein translocation from Agrobacterium into yeast and plant cells is studied to obtain fundamental insights in the translocation process

Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.

The aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds.

Streptomyces are bacteria abundant in soil that participate in diverse and complex interactions. These bacteria are the main producers of the antibiotics we currently use in the clinic.

The RNA guided endonuclease based on the CRISPR/Cas system of Streptococcus pyogenus is a potent new tool for genome engineering in plants.

Promotor: W. Jiskoot, Co-promotor: H.A. Every

This thesis describes the importance of being able to control the selectivity of potential drug candidates.

The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy.

Promotor: Prof.dr. M. Ubbink

The aim of the research presented in this thesis was to develop new methods forchallenging systems in liquid-state NMR using paramagnetic effects generated by thetwo-armed probe CLaNP-5.

Bacteria often experience external challenges, such as changes in environmental conditions or attacks by bacteriophages.

In this thesis, the researcher developed a nanosystem based on the metallophilic Interaction between cyclometalated complexes.

The work described in this thesis focuses on the development of linear or cyclized peptide probes against protein N-methyltransferases to characterize their specific binding behavior, providing further binding details for inhibitory activity study.

Supervisor: P.J.J. Hooykaas

Promotor: A. Kros

Promotor: Jaap Brouwer, Co-promotor: Remus Th. Dame

Promotor: A.P. IJzerman Co-promotor: L.H. Heitman

In photoactivated chemotherapy (PACT), a biologically active compound is caged by a light-cleavable protecting group.

Acute cardiovascular clinical events such as myocardial infarction and cerebral stroke represent the major cause of death in Western societies. These pathologies are primarily resulting from atherosclerosis, a progressive condition characterized by the accumulation of lipids, immune cells, and fibrous…

In this thesis, the role of plant-mediated soil legacy effects in shaping aboveground plant-insect interactions was investigated.

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-promotor: B.I. Florea

Cardiovascular disease is the leading cause of death worldwide.

Soil biodiversity is of great importance for the maintenance of multifunctionality in terrestrial ecosystems.

Promotor: A.P. IJzerman

Solar energy is used by photosynthetic organisms to drive energy required cellular processes. Is absorbed by two groups of pigments, located in the LHCs.

Marcellus Ubbink, hoogleraar Eiwitchemie, heeft een ECHO-subsidie van NWO ter waarde van 260 duizend euro in de wacht gesleept. Met het geld gaat hij onderzoek doen naar enzymremmers.

Low-mass main-sequence stars like our Sun are continuous sources of outflowing hot magnetised plasma. In the case of the Sun, this is known as the solar wind, whereas for other stars they are called stellar winds.

Synthesis and application of chemical biology tools to study immunomodulatory signaling lipids.