Bacteria often experience external challenges, such as changes in environmental conditions or attacks by bacteriophages.

Phospholipase A/acyltransferase 3 (PLAAT3) and PLAAT4 are enzymes involved in the synthesis of bioactive lipids. Despite sequential and structural similarities, the two enzymes differ in activity and specificity.

BlaC is the β-lactamase of Mycobacterium tuberculosis. We show that it can recover from inhibition by clavulanic acid and that phosphate helps it do so.

Promotor: Prof.dr. E.J.J. Groenen, Co-Promotor: M. Huber

Unfolding potential

Single-Molecule Microscopy (SMM) techniques constitute a group of powerful imaging tools that enable researchers to study the dynamic behavior of individual molecules.

In the thesis the dynamics of strategies is studied from two perspectives.In the first part of the thesis strategies are considered to be opinions present in a community.

Promotor: Prof.dr. M. Ubbink

Het Protein Centre kan u voorzien van gezuiverde eiwitten die essentieel zijn voor vele experimenten, variërend van in-vitro-inhibitorscreening en enzymatische analyse tot manipulatie van levende cellen.

This thesis describes the use of bioorthogonal proteins in immunological settings. It provides an introduction towards the field of protein modification, which was used throughout the thesis for the expression of proteins containing unnatural amino acids.

Veel kinderen leren niet op een niveau dat aansluit bij hun potentieel om te leren. Diagnostiek in de leerlingbeleiding zou daarom inzicht moeten geven in hoe een kind begeleid kan worden in het verder ontwikkelen van zijn of haar sterke kanten terwijl het tegelijkertijd handvatten biedt voor het ondersteunen…

Promotor: Prof.dr. M. Ubbink

Promotores: C.A.M.J.J. van den Hondel, V. Meyer, Co-promotor: A.F.J. Ram

Promotor: Prof.dr. M. Ubbink

Synthetic hydrogels that mimic the natural extracellular matrix in the biophysical and biochemical cues it provides to cells are in high demand, however the cell phenotypes as they are observed in vivo in numerous cases have yet to be attained.

The aim of the research presented in this thesis was to develop new methods forchallenging systems in liquid-state NMR using paramagnetic effects generated by thetwo-armed probe CLaNP-5.

Promotor: M. Ubbink, Co-promotor: G. Siegal

Due to their central physiological roles in living organisms, retaining beta-glycosidases have been the subject of tremendous research efforts to examine their structure/function relation using numerous biophysical and biochemical approaches.

Promotor: Prof.dr. S.F. Portegies Zwart

Promotor: Prof.dr. M. Ubbink, Co-promotor: G. Siegal

Promotor: W.Th.F. den Hollander

Promotor: W. Jiskoot, Co-promotor: A. Hawe

Supervisor: P.J.J. Hooykaas Co-Supervisor: R. Offringa

Promotor: J.W. van Holten

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

Promotor: Prof.dr. H.J.M. de Groot, Co-promotor: Francesco Buda

In our research protein translocation from Agrobacterium into yeast and plant cells is studied to obtain fundamental insights in the translocation process

An assessment approach that tailors to children’s instructional needs

In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.

Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.

Hoe beinvloed de structuur van één enkel gen de activiteit van genen die gevoelig zijn voor hormonen?

In this thesis, we focus on novel proteins/mechanisms that regulate integrin-adhesion mediated mechano-transduction.

Het artikel 'Flexible updating of dynamic knowledge structures' met bijdrage van Franziska Richter van Cognitieve Psychologie is uitgebracht door Nature.

The RNA guided endonuclease based on the CRISPR/Cas system of Streptococcus pyogenus is a potent new tool for genome engineering in plants.

Promotor: H.J. van den Herik, B.R. Katzy, Co-promotor: K. Sailer

Making a dynamic substance stock model in order to conduct scenario analyses and make forecasts.

Promotor: W. Jiskoot, Co-promotor: H.A. Every

This thesis describes the importance of being able to control the selectivity of potential drug candidates.

In the early universe, the dynamics of the Higgs field can give rise to many interesting phenomena.

Promotor: Promotor: W.Th.F. den Hollander, Co-promotor: V. Sidoravicius.

In previous studies at our laboratory it was demonstrated that drug exposure of HepG2 cells can lead to an altered TNFα-induced NF-κB oscillatory phenotype, concurrent with a synergistically increased sensitivity for TNFα-induced apoptosis.

The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy.

When a star gets too close to a supermassive black hole, it is torn apart by strong tidal forces in a tidal disruption event (TDE).

The work described in this thesis focuses on the development of linear or cyclized peptide probes against protein N-methyltransferases to characterize their specific binding behavior, providing further binding details for inhibitory activity study.

Supervisor: P.J.J. Hooykaas

The thesis describes the use of electron paramagnetic resonance (EPR) spectroscopy, in continuous wave and pulse modes, to address the interaction of α-Synuclein (αS) with membranes and the aggregation of αS.

Promotor: A. Kros

Promotor: Jaap Brouwer, Co-promotor: Remus Th. Dame

Hybrid zones occur where two species meet and produce offspring (hybrids). Typically, hybrids show a considerable reduction in fitness. In this thesis two hybrid zones are treated.

The intracluster medium (ICM) is a hot, tenuous and X-ray emitting gas that pervades galaxy clusters.