358 zoekresultaten voor “protein” in de Publieke website
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Protein Facility
Het Protein Centre kan u voorzien van gezuiverde eiwitten die essentieel zijn voor vele experimenten, variërend van in-vitro-inhibitorscreening en enzymatische analyse tot manipulatie van levende cellen.
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The advantages and disadvantages of bioorthogonal proteins
This thesis describes the use of bioorthogonal proteins in immunological settings. It provides an introduction towards the field of protein modification, which was used throughout the thesis for the expression of proteins containing unnatural amino acids.
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Morphogenesis and protein production in Aspergillus niger
Promotores: C.A.M.J.J. van den Hondel, V. Meyer, Co-promotor: A.F.J. Ram
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NMR studies of protein-small molecule and protein-peptide interactions
Promotor: M. Ubbink, Co-promotor: G. Siegal
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Spin-label EPR Approaches to Protein Interactions
Promotor: Prof.dr. E.J.J. Groenen, Co-Promotor: M. Huber
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NMR structural studies of protein-small molecule interactions
Promotor: Prof.dr. M. Ubbink, Co-promotor: G. Siegal
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Pharmaceutical Aspects of Subvisible Particles in Protein Formulations
Promotor: W. Jiskoot, Co-promotor: A. Hawe
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Biophysical characterization of membrane protein-small molecule interactions
Promotor: Prof.dr. M. Ubbink
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Activity-based protein profiling of diacylglycerol lipases
Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt
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Activity-based protein profiling in drug discovery
In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.
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Fluorescence Polarization Activity-Based Protein Profiling on Retaining Glycosidases
Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.
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Proteins in harmony: Tuning selectivity in early drug discovery
This thesis describes the importance of being able to control the selectivity of potential drug candidates.
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Classical and paramagnetic NMR spectroscopy applied to different protein systems
Promotor: Prof.dr. M. Ubbink
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Applications of paramagnetic NMR spectroscopy for protein research
The aim of the research presented in this thesis was to develop new methods forchallenging systems in liquid-state NMR using paramagnetic effects generated by thetwo-armed probe CLaNP-5.
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Dynamic organization of bacterial chromatin by DNA bridging proteins
Bacteria often experience external challenges, such as changes in environmental conditions or attacks by bacteriophages.
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Peptide-based probes for protein N-Methyltransferases
The work described in this thesis focuses on the development of linear or cyclized peptide probes against protein N-methyltransferases to characterize their specific binding behavior, providing further binding details for inhibitory activity study.
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PhD candidate to investigate a protein switch in photosynthesis
Wiskunde en Natuurwetenschappen, Leids Instituut voor Chemisch Onderzoek (LIC)
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Functional analysis of agrobacterium tumefaciens virulence protein VirD5
Supervisor: P.J.J. Hooykaas
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Parkinson’s protein α-synuclein: membrane interactions and fibril structure
The thesis describes the use of electron paramagnetic resonance (EPR) spectroscopy, in continuous wave and pulse modes, to address the interaction of α-Synuclein (αS) with membranes and the aggregation of αS.
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Postdoc candidate in biochemistry / cell glycobiology / protein targeted degradation
Wiskunde en Natuurwetenschappen, Leids Instituut voor Chemisch Onderzoek (LIC)
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Activity-based protein profiling of glucosidases, fucosidases and glucuronidases
Promotores: H.S. Overkleeft, G.A. van der Marel, Co-promotor: B.I. Florea
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Molecular engineering of plant development using Agrobacterium-mediated protein translocation
Supervisor: P.J.J. Hooykaas Co-Supervisor: R. Offringa
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Visualization of effector protein translocation from Agrobacterium tumefaciens into host cells
In our research protein translocation from Agrobacterium into yeast and plant cells is studied to obtain fundamental insights in the translocation process
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CRISPR Cas-induced targeted mutagenesis with Agrobacterium mediated protein delivery
The RNA guided endonuclease based on the CRISPR/Cas system of Streptococcus pyogenus is a potent new tool for genome engineering in plants.
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Supercritical carbon dioxide spray drying for the production of stable dried protein formulations
Promotor: W. Jiskoot, Co-promotor: H.A. Every
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Making the invisible visible: paramagnetic NMR and the transient protein complex
Promotor: Prof.dr. M. Ubbink
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Design, synthesis and application of paramagnetic probes for protein structure studies
The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy.
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Mesoporous silica nanoparticle-based protein delivery systems for biomedical applications
Promotor: A. Kros
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crenarchaeal chromatin: A biophysical characterization of chromatin proteins from Sulfolobus solfataricus
Promotor: Jaap Brouwer, Co-promotor: Remus Th. Dame
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Agunt Nisi Fixata: Ligand Receptor Binding Kinetics in G Protein-Coupled Receptors
The present thesis focuses on the pharmacological concept of drug-target interaction, which dates back to the beginning of modern pharmacology.
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Understanding protein complex formation: The role of charge distribution in the encounter complex
Protein–protein complexes are formed via transient states called encounter complexes that greatly influence the formation of the stereospecific complex.
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Structural characterization of bacterial proteins involved in antibiotic resistance and peptidoglycan biosynthesis
This thesis describes the structural and biochemical characterization of the β-lactamase BlaC from Mycobacterium tuberculosis (Mtb), and the Alr and YlmE proteins from Streptomyces coelicolor A3(2).Mtb is the main cause of tuberculosis.
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environmental cues: the adaptive qualities of chromatin compaction proteins
Promotor: M. Ubbink, Co-Promotor: R. T. Dame
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Exploring and exploiting activity-based protein profiling in chemical biology and medicinal chemistry
Overkleeft
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The use of activity based protein profiling to study proteasome biology
Promotor: H.S. Overkleeft, Co-Promotor: B.I. Florea
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SDS-PAGE at the nanoscale: A nanorecorder for single molecule protein sequencing with graphene
Schneider
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The role of the Bone Morphogenetic Protein Pathway in Colorectal Cancer
Promotie
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The activation mechanisms of G protein-coupled receptors: the case of the adenosine A2B and HCA2/3 receptors
Promotor: A.P. IJzerman
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Structural and functional analysis of proteins involved in natural product biosynthesis and morphological differentiation in Streptomyces
Streptomyces present a valuable platform for natural product discovery. Lugdunomycin is a novel angucycline-derived polyketide from Streptomyces sp QL37, with unprecedented skeleton and antimicrobial activity.
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Allosteric modulation by sodium ions and amilorides of G protein-coupled receptors
Promotor: A.P. IJzerman
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Allosteric modulation by sodium ions and amilorides of G protein- coupled receptors
Promotor: A.P. IJzerman
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and crystallographic studies of the Sso10a2 and human C1 inhibitor protein
Promotor: Prof.dr. J.P. Abrahams, Co-Promotor: N.S. Pannu
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space smarter: Artificial intelligence in drug design for G protein-coupled receptors
Over several decades, a variety of computational methods for drug discovery have been proposed and applied in practice. With the accumulation of data and the development of machine learning methods, computational drug design methods have gradually shifted to a new paradigm, i.e. deep learning methods…
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Identification and characterization of small molecule inhibitors of type VII protein secretion systems (1.0 fte)
Wiskunde en Natuurwetenschappen, Leids Instituut voor Chemisch Onderzoek (LIC)
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Essentiality of conserved amino acid residues in β-lactamase
Evolution acts via mutations in amino acid sequences. Substitution of essential amino acids leads to a nonfunctional protein. l.
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Dr. Marcellus Ubbink benoemd tot hoogleraar Protein Chemistry
Dr. Marcellus Ubbink is dit voorjaar bij de Faculteit Wiskunde en Natuurwetenschappen benoemd tot hoogleraar Protein Chemistry. Ubbink kreeg in 2008 een Vici-subsidie van NWO.
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The structure of the cytochrome P450cam-putidaredoxin complex determined by paramagnetic NMR spectroscopy and crystallography
Promotor: Prof.dr. M. Ubbink
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Analysis of sub-visible particles in complex injectable formulations
Promotors: W. Jiskoot; G.J. Witkamp, Co-promotor: M.R. Nejadnik
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Novel analytical approaches to characterize particles in biopharmaceuticals
Particles are omnipresent in biopharmaceutical products. In protein-based therapeutics such particles are generally associated with impurities, either derived from the drug product itself (e.g. protein aggregates), or from extrinsic contaminations (e.g. cellulose fibers).
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A chemical biology approach for targeting of ligand-drug conjugates
Promotores: H.S. Overkleeft, G.A. van der Marel, Co-Promotor: R.G. Boot