245 zoekresultaten voor “protein” in de Publieke website
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Protein Facility
Het Protein Centre kan u voorzien van gezuiverde eiwitten die essentieel zijn voor vele experimenten, variërend van in-vitro-inhibitorscreening en enzymatische analyse tot manipulatie van levende cellen.
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Activity-based protein profiling in drug discovery
In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.
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Activity-based protein profiling of diacylglycerol lipases
Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt
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Spin-label EPR Approaches to Protein Interactions
Promotor: Prof.dr. E.J.J. Groenen, Co-Promotor: M. Huber
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Morphogenesis and protein production in Aspergillus niger
Promotores: C.A.M.J.J. van den Hondel, V. Meyer, Co-promotor: A.F.J. Ram
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engineering of plant development using Agrobacterium-mediated protein translocation
Supervisor: P.J.J. Hooykaas Co-Supervisor: R. Offringa
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CRISPR Cas-induced targeted mutagenesis with Agrobacterium mediated protein delivery
The RNA guided endonuclease based on the CRISPR/Cas system of Streptococcus pyogenus is a potent new tool for genome engineering in plants.
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Fluorescence Polarization Activity-Based Protein Profiling on Retaining Glycosidases
Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.
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Pharmaceutical Aspects of Subvisible Particles in Protein Formulations
Promotor: W. Jiskoot, Co-promotor: A. Hawe
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Mesoporous silica nanoparticle-based protein delivery systems for biomedical applications
Promotor: A. Kros
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Parkinson’s protein α-synuclein: membrane interactions and fibril structure
The thesis describes the use of electron paramagnetic resonance (EPR) spectroscopy, in continuous wave and pulse modes, to address the interaction of α-Synuclein (αS) with membranes and the aggregation of αS.
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Visualization of effector protein translocation from Agrobacterium tumefaciens into host cells
In our research protein translocation from Agrobacterium into yeast and plant cells is studied to obtain fundamental insights in the translocation process
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Design and development of conformational inhibitors and activity-based probes for retaining glycosidases
Glycosidases are essential in fundamental biological processes and are responsible for the degradation of most (oligo)saccharides, glycolipids and glycoproteins.
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Applications of paramagnetic NMR spectroscopy for protein research
The aim of the research presented in this thesis was to develop new methods forchallenging systems in liquid-state NMR using paramagnetic effects generated by thetwo-armed probe CLaNP-5.
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Activity-based protein profiling of glucosidases, fucosidases and glucuronidases
Promotores: H.S. Overkleeft, G.A. van der Marel, Co-promotor: B.I. Florea
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Making the invisible visible: paramagnetic NMR and the transient protein complex
Promotor: Prof.dr. M. Ubbink
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Supercritical carbon dioxide spray drying for the production of stable dried protein formulations
Promotor: W. Jiskoot, Co-promotor: H.A. Every
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Design, synthesis and application of paramagnetic probes for protein structure studies
The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy.
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Classical and paramagnetic NMR spectroscopy applied to different protein systems
Promotor: Prof.dr. M. Ubbink
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Functional analysis of agrobacterium tumefaciens virulence protein VirD5
Supervisor: P.J.J. Hooykaas
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Marcellus UbbinkWiskunde en Natuurwetenschappen
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crenarchaeal chromatin: A biophysical characterization of chromatin proteins from Sulfolobus solfataricus
Promotor: Jaap Brouwer, Co-promotor: Remus Th. Dame
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Inhibitor Selectivity: Profiling and Prediction
Less than 1 in 10 drug candidates that enter phase 1 clinical trials actually gets approved for human use.
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Biodiesel production using blue-green cyanobacterium Synechococcus elongatus PCC 7942
Promotores: Prof.dr. C.A.M.J.J. van den Hondel, Prof.dr. V. Meyer (Technische Universität Berlin)
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Analysis of sub-visible particles in complex injectable formulations
Promotors: W. Jiskoot; G.J. Witkamp, Co-promotor: M.R. Nejadnik
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SDS-PAGE at the nanoscale: A nanorecorder for single molecule protein sequencing with graphene
Schneider
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Natural and non-natural factors influencing Alzheimer´s Aβ
Promotor: Prof.dr. J.P. Abrahams
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Cyclophellitol analogues for profiling of exo- and endo-glycosidases
To this day, all cyclophellitol-based inhibitors and ABPs have been close analogues of their natural substrate counterparts. As a result, these probes showed high selectivity towards their target glycosidases.
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and crystallographic studies of the Sso10a2 and human C1 inhibitor protein
Promotor: Prof.dr. J.P. Abrahams, Co-Promotor: N.S. Pannu
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Exploring and exploiting activity-based protein profiling in chemical biology and medicinal chemistry
Overkleeft
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PhD candidate to study the relationship between protein folding and metal-binding
Wiskunde en Natuurwetenschappen, Leids Instituut voor Chemisch Onderzoek (LIC)
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Hermen OverkleeftWiskunde en Natuurwetenschappen
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Structural characterization of bacterial proteins involved in antibiotic resistance and peptidoglycan biosynthesis
This thesis describes the structural and biochemical characterization of the β-lactamase BlaC from Mycobacterium tuberculosis (Mtb), and the Alr and YlmE proteins from Streptomyces coelicolor A3(2).Mtb is the main cause of tuberculosis.
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The use of activity based protein profiling to study proteasome biology
Promotor: H.S. Overkleeft, Co-Promotor: B.I. Florea
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Development of kinase inhibitors and activity-based probes
Promotor: H.S. Overkleeft, J. Neefjes, Co-promotor: M. van der Stelt
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environmental cues: the adaptive qualities of chromatin compaction proteins
Promotor: M. Ubbink, Co-Promotor: R. T. Dame
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A study on PsbS and its role as a pH sensor
Solar energy harnessed by plants and algae has great potential to be converted into biofuels for future generations.
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NMR structural studies of protein-small molecule interactions
Promotor: Prof.dr. M. Ubbink, Co-promotor: G. Siegal
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Biophysical characterization of membrane protein-small molecule interactions
Promotor: Prof.dr. M. Ubbink
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Enumeration and Simulation of Lattice Polymers as Models for Compact Biological Macromolecules
Promotores: H. Schiessel, G.T. Barkema
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Novel detectors and algorithms for electron nano-crystallography
Promotor: Prof.dr. J.P. Abrahams, Prof.dr. M. van Heel
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Activity-based proteomics of the endocannabinoid system
This thesis describes the use of an activity-based proteomics method to study the endocannabinoid system.
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NMR studies of protein-small molecule and protein-peptide interactions
Promotor: M. Ubbink, Co-promotor: G. Siegal
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protein-coupled receptors: the case of the adenosine A2B and HCA2/3 receptors
Promotor: A.P. IJzerman
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The structure of the cytochrome P450cam-putidaredoxin complex determined by paramagnetic NMR spectroscopy and crystallography
Promotor: Prof.dr. M. Ubbink
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modulation by sodium ions and amilorides of G protein- coupled receptors
Promotor: A.P. IJzerman
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Algorithms for the description of molecular sequences
Promotor: J.N. Kok, P.E. Slagboom Co-promotor: J.F.J. Laros
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A chemical biology approach for targeting of ligand-drug conjugates
Promotores: H.S. Overkleeft, G.A. van der Marel, Co-Promotor: R.G. Boot
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Fixata: Ligand Receptor Binding Kinetics in G Protein-Coupled Receptors
The present thesis focuses on the pharmacological concept of drug-target interaction, which dates back to the beginning of modern pharmacology.
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Dynamics of a β-lactamase
BlaC is the β-lactamase of Mycobacterium tuberculosis. We show that it can recover from inhibition by clavulanic acid and that phosphate helps it do so.