55 zoekresultaten voor “peptide n-glycanase” in de Publieke website
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Synthesis of cyclic peptides as bioconjugation platforms
Cyclic peptides are investigated as a platform to induce different orientations between various ligands.
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Rationally designed peptide based functional biomaterials
Promotor: Prof.dr. J.G.E.M. Fraaije, Co-promotor: A. Kros
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Synthetic modification of fusogenic coiled-coil peptides
A structural investigation of coiled coil peptides used as membrane fusogens, mimicing naturally occuring coiled-coil fusion proteins. Synthetic modifications have been made to alter lipid attachment, secondary structure and to insert photoactive azobenzene moieties for active control over coiled coil…
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Activity-based protein profiling of glucosidases, fucosidases and glucuronidases
Promotores: H.S. Overkleeft, G.A. van der Marel, Co-promotor: B.I. Florea
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Coiled-coil mediated liposomal fusion: Asymmetric behaving peptide fusogens
Membrane fusion is a vital process in living organisms and is mediated by zipper-like proteins.
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Gold nanoparticle-peptide conjugates for biomedical applications
Despite the fact that gold nanoparticles (GNPs) are one of the most studied nanoparticles, there is still a necessity for new approaches allowing for effective protective coating to enable wider use of GNPs in biomedical applications.
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Peptide-based probes for protein N-Methyltransferases
The work described in this thesis focuses on the development of linear or cyclized peptide probes against protein N-methyltransferases to characterize their specific binding behavior, providing further binding details for inhibitory activity study.
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Supramolecular peptide amphiphile nanoparticles as a novel allergy vaccine
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NMR studies of protein-small molecule and protein-peptide interactions
Promotor: M. Ubbink, Co-promotor: G. Siegal
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Exploring the chemical space of post-translationally modified peptides in Streptomyces with machine learning
The ongoing increase in antimicrobial resistance combined with the low discovery of novel antibiotics is a serious threat to our health care.
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Zipping into Fusion
Promotor: Prof.dr. J.G.E.M. Fraaije, Co-promotor: A. Kros
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Coiled-coils on lipid membranes: a new perspective on membrane fusion
Promotor: J.G.E.M. Fraaije, Co-Promotor: A. Kros
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Parkinson’s protein α-synuclein: membrane interactions and fibril structure
The thesis describes the use of electron paramagnetic resonance (EPR) spectroscopy, in continuous wave and pulse modes, to address the interaction of α-Synuclein (αS) with membranes and the aggregation of αS.
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Coiled-coil biomaterials for biological applications
This dissertation contains four works during my PhD. Different biomaterials have been designed based on coiled-coil peptides. These biomaterials have a range of applications, inclusing drug delivery, cell sorting to cell-cell fusion.
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Synthetic carbohydrate ligands for immune receptors
One of the main challenges in the development of an effective anti-cancer vaccine is the generation of an adequate and directed cellular immune response.
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How Electrostatic Interactions Drive Nucleosome Binding of RNF168 & PSIP1
The studies presented in the work show the potential of the integrative use of biophysical data in defining the structural basis of protein interactions.
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Spin-label EPR Approaches to Protein Interactions
Promotor: Prof.dr. E.J.J. Groenen, Co-Promotor: M. Huber
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Chemical tools to monitor and control proteasome activities
Promotores: H.S. Overkleeft; G.A. van der Marel Co-Promotor: B.I. Florea
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Self adjuvanting immunopeptides: Design and synthesis
Chapter 2 describes a post-synthetic methodology to introduce a fluorescent label in highly lipophilic, Pam3Cys based conjugates.
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PLGA-based particulate vaccine delivery systems for immunotherapy of cancer
Promotores: W. Jiskoot, F. Ossendorp
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In vitro and in vivo delivery of functionalized nanoparticles via coiled-coil interactions
Promotor: A. Kros, Co-promotor: R.R.C.L. Olsthoorn
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Natural and non-natural factors influencing Alzheimer´s Aβ
Promotor: Prof.dr. J.P. Abrahams
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Nieuwe techniek om notoir Alzheimer-eiwit te betrappen
Een nieuwe natuurkundige techniek maakt de weg vrij om het gedrag te bestuderen van Aβ-peptide. Het is bekend dat dit eiwit een rol speelt bij Alzheimer, maar voor effectieve medicatie is het nodig om te weten wat het precies doet. Publicatie op 16 maart in Journal of Biological Physics.
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Het immuunsysteem in actie tegen baarmoederhalskanker
In de zoektocht naar een vaccin tegen baarmoederhalskanker heeft bundeling van fundamentele kennis over het immuunsysteem en organische chemie al geresulteerd in een vaccin, dat nu wordt getest in klinische trials. Ondertussen wordt hard gewerkt aan een verbeterde variant.
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Dynamic hydrogels as synthetic extracellular matrices for three- dimensional cell culture
Synthetic hydrogels that mimic the natural extracellular matrix in the biophysical and biochemical cues it provides to cells are in high demand, however the cell phenotypes as they are observed in vivo in numerous cases have yet to be attained.
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Synthesis of cyclic peptides as bioconjugation platforms
Promotie
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Targeting Human Proteasomes: Substrates, Inhibitors and Prodrugs
Large parts of the research described in this Thesis aims at the development of oligopeptide-masked toxins and their in situ immunoproteasome-mediated activation.
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GMP-faciliteit voor innovatieve geneesmiddelen (KFT-J10 / IGFL)
De GMP-faciliteit maaktde ontwikkeling en productie van Advanced Therapy Medicinal Products (ATMP's) en chemische (peptide) synthese mogelijk.
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Systems pharmacology of the amyloid cascade
According to the amyloid cascade hypothesis, accumulation of beta-amyloid (Aβ) peptides initiates the pathological cascade in Alzheimer's disease (AD).
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Dyslipidemia at the crossroad of the skin barrier and the arterial wall
The research described in this thesis shows that hypercholesterolemia, a well-established risk factor for atherosclerosis, can impact skin lipid pool and barrier function already at young age. In the field of atherosclerosis, we showed that the small peptide Lyp-1 can be used as a targeting molecule…
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Chemical Biology Lezing: Biosynthesis and Engineering of Cyclic Peptide Antibiotics
Lezing
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Towards the development of synthetic vaccines against tuberculosis
The research described in this Thesis was aimed at designing and synthesizing nature-inspired compounds as part of TB vaccine discovery.
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Peptide-Based Probes for Protein N-Methyltransferases
Promotie
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A semisynthetic peptide-metalloporphyrin responsive matrix for artificial photosynthesis
Promotie
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Adjuvant-conjugated peptide vaccines for immunotherapy of cancer
Promotie
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Emerging approaches to study cell-cell interactions
The aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds.
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Liposomes as delivery system for allergen-specific immunotherapy
In this book liposomes are explored as delivery system for allergen-specific immunotherapy. Both cationic and anionic formulations are prepared with Bet v 1, one of the allergens in birch pollen.
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Coiled coil mediated liposome fusion: Asymmetric behaving peptide fusogens
Promotie
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Inverse electron demand Diels-Alder pyridazine elimination: synthetic tools for chemical immunology
The inverse electron demand Diels-Alder (IEDDA) pyridazine elimination emerged in 2013 as a new bioorthogonal reaction and constitutes a prime example of what is now known as dissociative bioorthogonal chemistry. The research described in this Thesis aims to develop synthetic strategies which enable…
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Stress-induced modulation of the innate immune system in cardiovascular disease
Promotor: Prof.dr. J. Kuiper
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Chemical Biology Lezing: The power of chemoselectivity - Functional peptide and protein-conjugates for proteomic and pharmaceutical research
Lezing
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Immune responses against conserved influenza epitopes: Lessons for peptide vaccination strategies
Promotie
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Mass Spectrometry-Based Degradomics Analysis of Toxoid Vaccines
The chemical and structural heterogeneity of toxoid vaccines makes their analysis challenging. However, detailed insights on a molecular level can be obtained by mass spectrometry.
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Niet tegen elke prijs
‘Toegepast biomedisch onderzoek zou moeten bijdragen aan verbetering van de kwaliteit van leven van de patiënt.’ Dat is een van de stellingen bij het proefschrift van Ilse Haisma. Promotie op 28 maart. Wat opvalt in de stelling van Ilse Haisma is wat er niet staat, namelijk dat toegepast medisch onderzoek…
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This Week’s Discoveries | 26 September 2017
Lezing
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Samenwerking start zoektocht voor nieuwe antibiotica met NWO beurs
Nieuwe stoffen identificeren om antibioticaresistente bacteriën te bestrijden. Onderzoekers van Leiden Universiteit en VU Amsterdam bundelen hun krachten om dit waar te maken, met een project gefinancierd door NWO’s Open Technologieprogramma (OTP). De samenwerking heeft bijna een miljoen euro toegekend…
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Alzheimer-eiwit betrapt op klonteren
Hersenziekten zoals de ziekte van Alzheimer gaan gepaard met opeenhopingen van eiwitten in de hersenen. Maar het verband met de ziekte is niet duidelijk. In het tijdschrift PhysChemPhys publiceren Martina Huber, Enrico Zurlo en collega's een nieuwe methode om het klonteren te volgen.
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Voorproeven van Nacht van Kunst & Kennis: Hoofdpijnpatiënten en 'migraineprof' Ferrari
Wetenschap en maatschappij ontmoeten elkaar in de Leidse Nacht van Kunst & Kennis op 20 september. Vooraf krijgen onze topsprekers alvast een prikkelende vraag. Is er nieuws over de oorzaak van migraine en wat zijn de nieuwste behandelmethoden?
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Sporen van bouwsteen van leven ontdekt rond jonge zonnen
In de nabijheid van zonachtige protosterren hebben wetenschappers licht opgevangen dat afkomstig is van het giftige gas methylisocyanaat. Als bouwsteen van biologisch belangrijke moleculen vormt het mogelijk een schakel in het ontstaan van leven.