Promotor: G.P. van Wezel, Co-Promotor: E. Vijgenboom

Hoe kan de techniek van cell wall engineering worden ingezet zodat de industrie streptomyceten beter kunnen verwerken tot nieuwe antibiotica?

Promotor: G.P. van Wezel, Co-promotor: D. Claessen

Promotores: C.A.M.J.J. van den Hondel, V. Meyer, Co-promotor: A.F.J. Ram

This Thesis describes the design, synthesis and evaluation as glycoprocessing enzyme inhibitors of focused libraries of iminosugars.

Bacteria are highly complex and diverse organisms that have adapted to survive in ecological niches ranging from the most extreme to the most heterogeneous environments.

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-Promotor: J.D.C. Codee

Factors affecting galanthamine production in Narcissus

Salicylic acid (SA) is a plant hormone, which is involved in the defense responses to pathogens and to abiotic stress, and in the regulation of plant growth and development.

Promotor: Prof.dr. G.P. van Wezel

Wiskunde en Natuurwetenschappen, Instituut Biologie Leiden (IBL)

Actinobacteria are Gram-positive bacteria that have a complex multicellular life cycle and are well known for their ability to produce a wide range of bioactive natural products (NPs).

The idea of citizens and public professionals collaborating to provide public services has widely spread in several countries around the globe. Co-production is often presented as the go-to solution for challenges like a legitimacy crisis of both the government and the market, and financial concerns.…

Promotores: Prof.dr. C.A.M.J.J. van den Hondel, Prof.dr. V. Meyer (Technische Universität Berlin)

Streptomyces bacteria are a valuable source of natural products, many of which are used in the clinic or in biotechnology.

This thesis examines the impacts and challenges of water use of electric power system. This thesis found that there are large differences in water use of electricity technologies, with hydropower and thermal power being two large water users.

Promotor: W. Jiskoot, Co-promotor: H.A. Every

Promotor: Prof.dr. M. Danhof

The studies described in this thesis deal with glycosidases, in particular alpha-galactosidases.

The growing demand of energy indicates that global energy resources in the form of fossil fuels will not be sufficient in the future. In order to solve potential future energy problems development of a sustainable hydrogen economy is highly desirable.

Filamentous Actinobacteria, such as Streptomyces, produce a plethora of chemically diverse bioactive metabolites that have found applications across medicine, agriculture and biotechnology.

Promotores: Prof.dr. G.W. Canters, Prof.dr. T.J. Aartsma

According to the amyloid cascade hypothesis, accumulation of beta-amyloid (Aβ) peptides initiates the pathological cascade in Alzheimer's disease (AD).

The filamentous bacteria Streptomyces are widespread inhabitants of terrestrial soils.

In this project innovative technology for photovoltaics cells is developed and analysis of ecological and health related risks are explicitly accounted for.

The electrochemical oxygen reduction reaction (ORR) is an essential half-reaction for the utilization of hydrogen as a sustainable fuel, via the conversion of hydrogen to electrons and protons facilitated by the ORR. In the most common fuel cells, the ORR is requires high loadings of non-abundant platinum…

Promotors: Prof.dr. P.J. Punt, Prof.dr. C.A.M.J.J. van den Hondel

An objective of this research was to achieve direct, well-defined and non-rate-limiting electron transfer between respiratory enzymes and the electrode surface by means of 'Q-wires'.

Phospholipase A/acyltransferase 3 (PLAAT3) and PLAAT4 are enzymes involved in the synthesis of bioactive lipids. Despite sequential and structural similarities, the two enzymes differ in activity and specificity.

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-promotor: B.I. Florea

Streptomyces are filamentous bacteria that produce more than two-thirds of known antibiotics.

This thesis describes the structural and biochemical characterization of the β-lactamase BlaC from Mycobacterium tuberculosis (Mtb), and the Alr and YlmE proteins from Streptomyces coelicolor A3(2).Mtb is the main cause of tuberculosis.

Glycoside hydrolases (glycosidases/GHs) are widely abundant enzymes in all kingdoms of life and are important biocatalysts that catalyze the hydrolysis of glycosidic linkages in oligo/polysaccharides, glycoproteins and glycolipids with tremendous efficiency

Promotor: B.van de Water, Co-promotor: L.S.Price

Vitamin A or retinol is essential in embryonic development, the visual cycle and the immune system.

The recently introduced nature-originated deep eutectic solvents, so-called natural deep eutectic solvents (NADES) are considered as truly green solvents, which composed of natural ingredients found abundantly in organisms.

Sphingolipids are important membrane compounds with a variety of functions. In mammalian cells, different enzymes are involved in the metabolism of sphingolipids, but interruption of this metabolism process leads to different diseases.

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

Monoacylglycerol lipase (MAGL) is the principal enzyme responsible for hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-AG). MAGL inhibition provides several potential therapeutic opportunities, including anti-nociceptive, anti-inflammatory and anti-cancer activity.

In this thesis, the discovery and optimization is described of chemical tools to study the N-acylethanolamine (NAE) biosynthetic pathway.

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

Plants are colonized by an astounding number of microorganisms that can provide different life-support functions, including nutrient acquisition and protection against (a)biotic stresses like drought or pathogen attack.

The soil-dwelling, filamentous bacteria of the genus Streptomyces are renowned for their production of useful secondary metabolites including antibiotics. The work described in this thesis provides new insights on the role and regulation of antibiotic production and resistance in these bacteria.

Aspergillus niger is an important industrial producer of organic acids and enzymes producing large amounts of spent fungal biomass.

Almost all bacteria are enveloped by a cell wall that provides cellular protection.

Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.

Bacterial biocatalysis constitutes a sustainable alternative for high-value chemicals production by enabling the utilization of renewable feedstocks.