Bacterial chemosensory arrays are protein assemblies that are the key structural and functional component for motile bacteria to sense their internal or environmental chemical signals.

Inverse problems are problems where we want to estimate the values of certain parameters of a system given observations of the system. Such problems occur in several areas of science and engineering. Inverse problems are often ill-posed, which means that the observations of the system do not uniquely…

Cryogenic electron microscopy (cryo-EM) is a powerful technique used to visualize the inside of cells and to study specific protein complexes. Within this thesis, I describe the use of various cryo-EM techniques to gain insight into the structural changes of the human pathogen, Vibrio cholerae, as it…

The research in this thesis is focused on tomographic reconstruction based on two imaging modalities in electron microscopy.

Promotor: R. Tijdeman, Co-promotor: H.J.J. te Riele

Promotores: K.J. Batenburg, B. Koren

Promotores: R. Tijdeman, K.J. Batenburg

Promotor: S.J. Edixhoven, Co-promotor: K.J. Batenburg

Promotor: K.J. Batenburg

Electron microscopy has become an extremely important techniquein a wide variety of elds.

Optical projection tomography (OPT) is a tomographic 3D imaging technique used for specimens in the millimetre scale.

Single-molecule spectroscopy has become a powerful method for using organic fluorescent molecules in numerous applications.

In condensed matter systems electron-electron interactions, negligible in everyday metals, can dramatically alter the electronic behavior of the system. Examples of such altered behavior include high-temperature superconductivity and modulation of the electron density.

This thesis describes a study from both a theoreticaL and an experimental point of view.

Bacteria have the ability to alter their morphology in order to adapt to changing environments.

Promotor: Prof.dr. J.P. Abrahams, Prof.dr. M. van Heel

Het doel van deze faciliteit is om het biomedisch onderzoek te versnellen door toegang te bieden tot geavanceerde licht- en elektronenmicroscopie en door het delen van expertise over monstervoorbereiding, beeldvormingsmodaliteiten, gegevensopslag en beeldanalyse.

Promotor: Prof.dr. J.M. van Ruitenbeek, Prof.dr. R.M. Tromp

Materials with strongly correlated electrons show some of the most mysterious and exotic phases of quantum matter, such as unconventional superconductivity, quantum criticality and strange metal phase.

The dissertation describes TEM experiments on heterogeneous catalysts.

n this PhD thesis, the recombination of different atomic lattices in stacked 2D materials such as twisted bilayer graphene is studied. Using the different possibilities of Low-Energy Electron Microscopy (LEEM), the domain forming between the two atomic layers with small differences is studied.

Promotor: J. Zaanen, Co-Promotor: V. Juricic

Promotores: G.W. Canters, T.J. Aartsma

Promotor: J.P. Abrahams, Co-promotor: T. Grüne

Promotores: Prof.dr. H.P. Spaink & Prof.dr. P.C.W. Hogendoorn Co-promotor: Dr. M.J.M. Schaaf

The inverse electron demand Diels-Alder (IEDDA) pyridazine elimination emerged in 2013 as a new bioorthogonal reaction and constitutes a prime example of what is now known as dissociative bioorthogonal chemistry. The research described in this Thesis aims to develop synthetic strategies which enable…

Promotie

With tomography it is possible to reconstruct the interior of an object without destroying.

This dissertation is an experimental study of laser-generated, atmospheric pressure, transient toroidal helium plasmas.

Tomography is a powerful technique to non-destructively determine the interior structure of an object.Usually, a series of projection images (e.g.\ X-ray images) is acquired from a range of different positions.

Promotie

In this thesis the combinatorial use of bioorthogonal labelling and Electron Microscopy (EM)-based imaging techniques is explored to enable observations of specific molecular targets in their ultrastructural context within the cell.

Een speciaal type microscoop jaagt momenteel een revolutie aan in de biologie: de cryo-elektronenmicroscoop. Twee van zulke giganten staan sinds 2011 op de Leidse Faculteit der Wiskunde en Natuurwetenschappen. Hoogleraar Ultrastructuurbiologie Ariane Briegel vertelt hierover in het NRC Handelsblad.

Promotor: K.J. Batenburg, Co-promotor: A.W. van der Vaart, B. de Smit

Met zijn presentatie tijdens het Nederlands Mathematisch Congres over nieuwe tomografie-technieken heeft Joost Batenburg, onderzoeker bij de Universiteit Leiden en bij het Centrum voor Wiskunde en Informatica (CWI) in Amsterdam, de eerste Philips Mathematics Prize voor promovendi gewonnen.

Promotor: Prof.dr. K.J. Batenburg

Het logo van de Universiteit Leiden, met letters zo klein als een bacterie. Wetenschappers van het LUMC en het Instituut Biologie hebben het kleinste logo ooit van onze universiteit gemaakt. Een grapje met een serieuze ondertoon: met de nieuwe microscoop waarmee het logo is gemaakt, kunnen zij nog gedetailleerder…

Artificial photosynthesis has recognised potential to produce green and sustainable fuels from earth-abundant resources such as water, carbon dioxide (CO2), and sunlight.

Promotores: Prof.dr. D. Bouwmeester, Prof.dr. A. Fiore (TU Eindhoven)

The thesis describes the use of electron paramagnetic resonance (EPR) spectroscopy, in continuous wave and pulse modes, to address the interaction of α-Synuclein (αS) with membranes and the aggregation of αS.

Promotor: Prof.dr. E.J.J. Groenen, Co-Promotor: M. Huber

De Nobelprijs voor Scheikunde 2017 is uitgereikt aan Jacques Dubochet, Richard Henderson en Joachim Frank, voor hun ontwikkeling van de cryo-elektronenmicroscopie. Met deze techniek kunnen biomoleculen in 3D in beeld gebracht worden, legt hoogleraar Ultrastructuurbiologie Ariane Briegel uit.

Multicellular giant algae Chara species have been widely used in physiological studies for decades.

Een internationaal team met onderzoekers uit Leiden heeft ontdekt hoe een bacterie een intern systeem heeft herschikt om grip op beweging te krijgen. Gecontroleerde beweging is heel belangrijk bij het binnendringen van een gastheer, met mogelijk ziekte tot gevolg. Publicatie op 27 april in Nature Co…

Promotor: Prof.dr. E. Eliel, Co-Promotor: M.J.A. de Dood

Promotors: Prof.dr. J. Zaanen, Prof.dr.ir. H. Hilgenkamp

Atoms and molecules are the basic units of matter. If we keep dividing a bar of gold or a glass of water into smaller parts, at the end we are left with a single gold atom or a water molecule.

In this thesis, bioorthogonal chemistry is combined with correlative light-electron microscopy to selectively label and study pathogenic intracellular bacteria within the host immune cell.

Promotor: Prof.dr. H.J.M de Groot, Co-Promotor: A. Alia