Promotor: B. vd Water, T. Schmidt, Co-Promotor: E.H.J. Danen

The main aim of this thesis was to unravel the signaling and regulatory networks that drive tumor cell migration during breast cancer metastasis.

This thesis describes our search to identify and understand possible regulatory mechanisms of integrin α6β4 in cell-matrix adhesion and intracelular signaling.

The aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds.

In het Cell Observatory zoomen we in op levende cellen. We bestuderen de processen van het leven op moleculair niveau en onderzoeken bijvoorbeeld hoe we de uitzaaiing van kanker tegen kunnen gaan en medicijnen kunnen ontwikkelen tegen ziekten zoals tuberculose. Medicijnontwikkeling is één van de kernactiviteiten…

Mechanical interactions between cells and their environment play an important role in many biological processes.

Wiskunde en Natuurwetenschappen, Leiden Academic Centre for Drug Research (LACDR)

Cardiovascular disease is a major global burden and atherosclerosis is the main underlying pathological process.

Promotor: Prof.dr. J. Kuiper

Promotie

In this thesis, we used genetically engineered mouse models and a variety of cell-culture based assays to identify genes and pathways that are involved in the development and treatment of invasive lobular carcinoma (ILC).

A new type of tripodal squaramide-based supramolecular hydrogels is developed and studied.

Traditional drug discovery approaches have been hampered by (in vitro) cell-culture models that poorly represent the situation in the human body.

During embryonic growth, cells proliferate, differentiate, and collectively migrate to form different tissues at the right position and time in the body.

Almost all bacteria are enveloped by a cell wall that provides cellular protection.

Aspergillus niger is an important industrial producer of organic acids and enzymes producing large amounts of spent fungal biomass.

There is an urgent need for more physiologically relevant cell culture methods to guide compound selection in pre-clinical stages of the drug development pipeline.

In this research project, we aimed to obtain more insight in the role of mast cells in the immune driven disorders rheumatoid arthritis and atherosclerosis.

This thesis describes the design and synthesis of fragments of various cell wall carbohydrates of the Streptococcus species, including the branched Group B-specific antigen (GBC) of Group B Streptococcus, glycerol phosphate (GroP) modified group A carbohydrate (GAC), and the O-acetylated type 1 capsular…

CONCLUSION: This study demonstrates that human macrophages can be induced to exert direct anti-tumor activity against osteosarcoma cells. Our observation that the induction of macrophage anti-tumor activity by L-MTP-PE required IFN-gamma may be of relevance for the optimization of L-MTP-PE therapy in…

In order to gain a better insight into the basis of the rapid jasmonic acid (JA)-mediated stress response, an integrated approach using a targeted high-resolution mass spectrometry-based (HRMS) platform, was conducted to monitor the metabolism of JA using the model system of cell suspension cultures…

Bacteria have the ability to alter their morphology in order to adapt to changing environments.

Promotor: Prof.dr. T. Schmidt

In this thesis, we used genetically engineered mouse models to identify genes and pathways that are involved in ILC formation and in the development of resistance to FGFR-targeted therapy.

Promotores: Prof.dr. G.F.A. Kersten, W. Jiskoot, Co-promotor: J-P. Amorij

Cytotoxic T-cells (CTLs) are involved in the clearance of viruses and killing of tumor cells.

Promotor: C.A.J. Knibbe, Co-promotor: J.J. Boelens, R.G.M. Bredius

Streptomyces are multicellular, Gram-positive bacteria in the phylum of actinobacteria which produce a high amount of bioactive natural products of which the expression is tightly coordinated with the life cycle.

Promotor: Prof.dr. R. Verpoorte, Co-Promotores: N.R. Mustafa, A.E. Schulte

Light-Harvesting Complex II (LHCII) is responsible for light absorption and excitation energy transfer in plants and photosynthetic algae, while in high light it undergoes conformational changes by which it quenches excitations to prevent photodamage.

Promotie

Promotor: A.H. Meijer, Co-promotor: B.E. Snaar-Jagalska

In this thesis, the focus lies on studying glucocorticoid receptor dynamics in living cells with the aim of understanding how this transcription factor finds its DNA target sites to regulate transcription.

In this thesis, we aim to shed light on the diverse and often opposing roles of integrin α3β1 in cancer.

Promotor: G.A. van der Marel, Co-promotor: J.D.C. Codée

Details kunnen zien van een zieke of gezonde cel, dat is mogelijk met de supermicroscoop van het consortium Cyttron. Niet alleen de microscoop is vernieuwend in zijn soort: ook de vorm waarop de partijen samenwerken past in de huidige tijd. “Toegang tot elkaars techniek, daar draait het om in ons samenwerkingsverband”,…

Promotor: T. Schmidt

Met de nodige maatregelen starten onderzoekers hun werkzaamheden in verschillende laboratoria weer deels op. Het Leidse Cell Observatory is één van de plekken waar wetenschappers sinds 11 mei hun labwerk hervatten.

Promotor: Prof.dr. G.P. van Wezel

Promotor: Prof.dr. J. Kuiper

Promotie

Promotores: B. van de Water; P. Hogendoorn; J. Bovée Co-Promotor: E.H.J. Danen

Promotor: Prof.dr. R.M.H. Merks

Scattering of light in the presence of nano-structured materials, i.e. with features in the order of the wavelength of the light or smaller, reveals details of how light interacts with matter at the nanoscale.

Promotor: Prof.dr. H.P. Spaink, Co-promotor: Dr. B.E. Snaar

Streptomyces present a valuable platform for natural product discovery. Lugdunomycin is a novel angucycline-derived polyketide from Streptomyces sp QL37, with unprecedented skeleton and antimicrobial activity.

Bacterial biocatalysis constitutes a sustainable alternative for high-value chemicals production by enabling the utilization of renewable feedstocks.

Decentralized plug and play systems for energy production are the future picture of our society. Artificial photosynthetic systems are used for this purpose.