Promotor: Prof.dr. H.S. Overkleeft

De verhoogde activatie van het striatum, die adolescenten laten zien bij beloning, wordt meestal in verband gebracht met risicogedrag en negatieve gezondheidseffecten. In dit artikel in Nature Communications wordt een mogelijke positieve kant van die verhoogde activatie onderzocht. De activatiepiek…

This thesis describes the use of an activity-based proteomics method to study the endocannabinoid system.

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-promotor: B.I. Florea

In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.

Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.

Promotor: H.S. Overkleeft, J. Neefjes, Co-promotor: M. van der Stelt

This thesis provides an overview of the development of the Pair Configuration to Molecular Activity Coefficient (PAC-MAC) model.

Glycosidases are essential in fundamental biological processes and are responsible for the degradation of most (oligo)saccharides, glycolipids and glycoproteins.

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

Lysosomal glycosidases are acid hydrolases that fragment glycoconjugates in lysosomes.

Phospholipase A/acyltransferase 3 (PLAAT3) and PLAAT4 are enzymes involved in the synthesis of bioactive lipids. Despite sequential and structural similarities, the two enzymes differ in activity and specificity.

Promotor: H.S. Overkleeft, Co-Promotor: B.I. Florea

Overkleeft

The research described in this thesis focused on the preparation of S/N functionalized carbene ligands and their transition metal complexes, and the exploration of their application as electrocatalysts for proton reduction.

Describe and identify what dosimetry parameters are of importance to interpret dose-response relationships (eg., mortality, sub-lethal, growth or reproduction inhabitation, DNA damage and reactive oxygen species, etc. ) for metal-based nanoparticles? How to develop quantitative models that enable to…

Plant architecture has distinct forms in different plant species, but also within a species the finalarchitecture of a plant is determined by its gradual development and changes therein induced by environmental conditions during the plant’s life cycle.

Glycoside hydrolases (glycosidases/GHs) are widely abundant enzymes in all kingdoms of life and are important biocatalysts that catalyze the hydrolysis of glycosidic linkages in oligo/polysaccharides, glycoproteins and glycolipids with tremendous efficiency

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

De Young Academy Leiden (YAL) heeft een 444 Interdisciplinary Activity Grant toegekend aan Anna van Duijvenvoorde en Stephanie Rap voor het organiseren van een seminar op het gebied van jongvolwassenen, neurowetenschappen en het recht.

Alex Brandsen en Wouter Verschoof-van der Vaart, promovendi bij de onderzoeksgroep Digital Archaeology en het Data Science Research Programme, hebben een 444-beurs van de Young Academy Leiden ontvangen. Hiermee gaan ze in Oxford een sessie houden om de samenwerking te verbeteren tussen onderzoekers…

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

Promotor: Prof.dr. G.P. van Wezel

Vitamin A or retinol is essential in embryonic development, the visual cycle and the immune system.

The chemical synthesis of inhibitors and probes targeting endo-glycosidases.

Vancomycin is a last-resort antibiotic for the treatment of many Gram-positive bacterial infections, while remaining inactive against Gram-negative strains.

How presence, abundance and enzymatic activities of AM and EM fungi differently affect soil carbon sequestration processes?

CONCLUSION: This study demonstrates that human macrophages can be induced to exert direct anti-tumor activity against osteosarcoma cells. Our observation that the induction of macrophage anti-tumor activity by L-MTP-PE required IFN-gamma may be of relevance for the optimization of L-MTP-PE therapy in…

The recently introduced nature-originated deep eutectic solvents, so-called natural deep eutectic solvents (NADES) are considered as truly green solvents, which composed of natural ingredients found abundantly in organisms.

The work described in this thesis focuses on the development of linear or cyclized peptide probes against protein N-methyltransferases to characterize their specific binding behavior, providing further binding details for inhibitory activity study.

In this thesis an activity-based probe was discovered that could visualize the activity of PLAATs. With an optimized gel-based ABPP assay in hand, screening of a compound library led to the discovery of alpha-ketoamides as a hit for PLAAT3.

Advanced sensing techniques require graphene with high quality and well-controlled surface chemistry.

Prof.dr. J. Memelink

Less than 1 in 10 drug candidates that enter phase 1 clinical trials actually gets approved for human use.

In this thesis, the discovery and optimization is described of chemical tools to study the N-acylethanolamine (NAE) biosynthetic pathway.

Monoacylglycerol lipase (MAGL) is the principal enzyme responsible for hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-AG). MAGL inhibition provides several potential therapeutic opportunities, including anti-nociceptive, anti-inflammatory and anti-cancer activity.

How soil microbial composition impacts structure and composition of the aboveground plant and animal communities?

Lees hier alles over de verschillende projecten van de Young Academy Leiden.

To this day, all cyclophellitol-based inhibitors and ABPs have been close analogues of their natural substrate counterparts. As a result, these probes showed high selectivity towards their target glycosidases.

In this thesis, we have studied the potential of the zebrafish larval model in studying the ECS, as a complementary model to the existing rodent models.

In this dissertation iron-based homogeneous catalysts were synthesized, characterized and investigated for water oxidation activity.

Streptomyces present a valuable platform for natural product discovery. Lugdunomycin is a novel angucycline-derived polyketide from Streptomyces sp QL37, with unprecedented skeleton and antimicrobial activity.

In 2019 bestond de Universiteit Leiden 444 jaar. Deze bijzondere leeftijd vroeg om een bijzondere viering, samen met de inwoners van Leiden en Den Haag. In, met en voor de stad.

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

In this dissertation, the synthesis and characterization of a series of iron complexes based on different ligand platforms are described.