Climate change is a challenge for both current and future generations. New biological resources have to be developed in order to meet the demand for energy as well as the demand for food.

Nuclear Magnetic Resonance (NMR) Spectroscopy is a non-destructive analytical technique that is used to study the nature and characteristics of molecules with an atomic level precision. It has a wide range of applications especially in synthetic chemistry, biological and biochemical research groups…

NMR theory can be tough. Luckily, most NMR theory can be visualized to aid you in understanding. The animations below show precession with a 180 degree pulse, J-coupling and T1/T2 relaxation.

The aim of the research presented in this thesis was to develop new methods forchallenging systems in liquid-state NMR using paramagnetic effects generated by thetwo-armed probe CLaNP-5.

Promotor: M. Ubbink, Co-promotor: G. Siegal

Nature uses a special class of histone proteins, histone variants, to modulate the properties of chromatin at defined genomic locations.

Zebrafish is an excellent model organism for studying various human diseases. Due to opaqueness of the adult phase, in vivo studies are restricted to early embryonic stages. This raises the need for rapid sensitive and non-invasive in vivo imaging methods to follow developmental processes, not only…

Dr. in Biomedical NMR spectroscopy, LUMC, Leiden - The Netherlands

Promotor: Prof.dr. M. Ubbink

Hass

Multicopper oxidase, laccase, can efficiently reduce oxygen to water and are mostly used in the enzymatic biofuel cells.

Promotor: Prof.dr. M. Ubbink

Promotor: Prof.dr. M. Ubbink

Promotor: M. Ubbink, Co-promotor: G. Siegal

Huub de Groot is professor in Biophysical Organic Chemistry. With his team he works in the field of photosynthesis and artificial photosynthesis. The molecular basis for photosynthesis is formed by protein complexes and organelles that contain chlorophyll molecules. The antenna systems herein capture…

Promotores: Prof.dr. H.J.M. de Groot, Prof.dr. J. Matysik (Universitaet Leipzig)

Light-Harvesting Complex II (LHCII) is responsible for light absorption and excitation energy transfer in plants and photosynthetic algae, while in high light it undergoes conformational changes by which it quenches excitations to prevent photodamage.

A network combining 9 academic research groups and 4 collaborating industrial companies will train the next generation of PhD students and post-doctoral researchers, in developing and applying novel experimental and theoretical methods in the NMR spectroscopy of systems containing paramagnetic metals.…

The National Roadmap for Large-Scale Research Facilities programme of NWO enables the building or renovation of research facilities with international allure. Leiden NMR groups take part in the uNMR-NL consortium coordinated by Marc Baldus (Utrecht University) that has been awarded almost 18 million…

Due to their central physiological roles in living organisms, retaining beta-glycosidases have been the subject of tremendous research efforts to examine their structure/function relation using numerous biophysical and biochemical approaches.

Promotor: Prof.dr. R. Verpoorte, Co-promotores: Dr. Y.H.Choi, N.R. Mustafa

Paramagnetism. An obscure occult practice? No. It’s one of the newest scientific methods for mapping the behaviour of proteins with great precision. Marcellus Ubbink has been awarded 3 million euro by NWO and Leiden University to purchase a very powerful instrument. The goal: to acquire greater insight…

PURPOSE: To determine the effect of elasticity on the skin permeation of ketoprofen from surfactant-based vesicular formulations and elastic liposomes.

Factors affecting galanthamine production in Narcissus

Phospholipase A/acyltransferase 3 (PLAAT3) and PLAAT4 are enzymes involved in the synthesis of bioactive lipids. Despite sequential and structural similarities, the two enzymes differ in activity and specificity.

In this thesis, we show how MRFM can usefully contribute to the field of condensed-matter.

The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy.

Promotors: Prof.dr. P.G.L. Klinkhamer, Prof.dr. P.M. Brakefield

Researchers from the Leiden Institute of Chemistry show proof of principle that live-cell structural changes and metabolic processes can be followed in real time with NMR spectroscopy. They performed their study on photosynthetic green algae that are metabolically flexible and can sustain energy generation…

Decentralized plug and play systems for energy production are the future picture of our society. Artificial photosynthetic systems are used for this purpose.

First author Rubin Dasgupta, PhD student at the Biophysical Organic Chemistry & Macromolecular Biochemistry groups at the Leiden Institute of Chemistry has made the cover of the June edition of Solid State Nuclear Magnetic Resonance.

Protein–protein complexes are formed via transient states called encounter complexes that greatly influence the formation of the stereospecific complex.

Promotor: G.P. van Wezel, Co-promotor: Y.H. Choi

Promotor: H.J.M. de Groot, Co-Promotor: A. Alia

Promotor: Prof.dr. G.P. van Wezel

Promotor: T.H. Oosterkamp

BlaC is the β-lactamase of Mycobacterium tuberculosis. We show that it can recover from inhibition by clavulanic acid and that phosphate helps it do so.

A new NMR microscope gives researchers an improved instrument to study fundamental physical processes. It also offers new possibilities for medical science, for example to better study proteins in Alzheimer patients’ brains. Publication in Physical Review Applied.

Dr. at Max Planck Institute for chemical Ecology, Jena - Germany

A full overview of MacBio PhD Theses.

The studies presented in the work show the potential of the integrative use of biophysical data in defining the structural basis of protein interactions.

Anthe Janssen graduated ‘summa cum laude’ for the master’s programme Chemistry.He used NMR methods to gain insight in the syntheses and behavior of bio sugars. With this knowledge future therapeutics and research tools in hemical and medicinal biology can be developed. Anthe was awarded € 2.500.

Two things are needed to produce fuel from sunlight: an antenna that harvests light, and a light-driven catalyst. The most efficient antennae contain bacteria. An international team headed by Huub de Groot imitated them and discovered how they function.

Research at the BPOC/SSNMR group is comprised of the following research themes:

Contact Biophysical Organic Chemistry/Solid State NMR

Plants, algae and cyanobacteria have the amazing capacity to perform delicate photophysical and photochemical processes of light capture, excitation transfer, charge separation and catalysis in fluctuating living environments. As a consequence, photosynthetic proteins and membranes form dynamic architectures.…