253 zoekresultaten voor “enzyme” in de Publieke website
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Regulation of enzyme production in fungal cell factories
Our object is to identify and characterize transcription factors involved in lignocellulosic biomass degradation in the filamentous fungus Aspergillus niger.
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Iminosugars as glucosylceramide processing enzymes inhibitors: design, synthesis and evaluation
This Thesis describes the design, synthesis and evaluation as glycoprocessing enzyme inhibitors of focused libraries of iminosugars.
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Postdoctoral researcher in structural biology of glycan-modifying enzymes
Wiskunde en Natuurwetenschappen, Leids Instituut voor Chemisch Onderzoek (LIC)
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The synthesis of mannose-derived bioconjugates and enzyme inhibitors
Promotores: H.S. Overkleeft, G.A. van der Marel, Co-Promotor: J.D.C. Codee
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Controlling growth and morphogenesis of the industrial enzyme producer Streptomyces lividans
Promotor: Prof.dr. G.P. van Wezel, Co-Promotor: E. Vijgenboom
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Novel approaches to develop filamentous micro-organisms for enzyme production (FILAZYME)
Can we develop new enzymes and cell factories to upgrade current enzyme cocktails towards complete degradation of biomass?
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filamentous fungus Aspergillus niger as cell factory for starch degrading enzymes
We aim to develop A. niger strains that produce more starch degrading enzymes even in the absence of starch.
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Molecular characterization of copper-dependent enzymes involved in Streptomyces morphology
Promotor: G.P. van Wezel, Co-promotor: D. Claessen
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PROTEOMINING: a novel proteomics-based pipeline for drug and enzyme discovery in filamentous actinomycetes
Can a new proteomics workflow be developed to link genes and gene clusters to bioactive molecules, identify novel compounds and enhance the production in the Streptomyces lividans enzyme?
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is more: reduced mycelial heterogeneity for improved production of enzymes and antibiotics
How can cell wall engineering approaches be used to improve streptomycetes for industry to make new antibiotics?
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Exploring novel regulators and enzymes in salicylic acid-mediated plant defense
Salicylic acid (SA) is a plant hormone, which is involved in the defense responses to pathogens and to abiotic stress, and in the regulation of plant growth and development.
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Small-molecule tools to study human cysteine enzymes SENPs and PARK7
Promotie
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PhD candidate || Structure and dynamics in enzyme catalysis (1.0 fte)
Wiskunde en Natuurwetenschappen, Leids Instituut voor Chemisch Onderzoek (LIC)
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Protein motions revealed by paramagnetic NMR spectroscopy
Hass
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Identification and characterization of inhibitors of carbohydrate modifying enzymes to generate a new type of anti-bacterial molecule (1.0 fte)
Wiskunde en Natuurwetenschappen, Leids Instituut voor Chemisch Onderzoek (LIC)
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to identify and characterize inhibitors of carbohydrate modifying enzymes to generate a new type of anti-viral molecule
Wiskunde en Natuurwetenschappen, Leids Instituut voor Chemisch Onderzoek (LIC)
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Minrui Ren
Wiskunde en Natuurwetenschappen
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physiological conditions and in relation to angiotensin-converting enzyme inhibition
Promotor: Prof.dr. M. Danhof
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Streptomyces as cell factories
We aim to engineer streptomycetes to fully exploit their potential for natural product productions, by a rational design and evolution approach.
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European collaboration shines a light on enzyme discovery for industry
A European consortium has provided a disruptive technological breakthrough to allow the discovery and characterization of novel enzymes for industrial biotechnology. The technology will open the way to more efficient industrial processes such as in the biofuel, animal feed and paper and pulp industr…
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Marcellus Ubbink receives grant for research into evolution of enzymes
The Dutch organisation for scientific research NWO has announced that it will award an ENW-Klein grant to Marcellus Ubbink (LIC) for his research into the role of temperature in the evolution of enzymes. Sixteen grants have been awarded in total.
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Arthur Ram receives NWO grant to improve enzyme factory
This year, microbiologist Arthur Ram will start new research on producing useful enzymes in bulk. Ram receives an NWO grant for this project that will find its applications in the industrial biotechnology and food industry.
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Changing enzymes for clean energy and disease prevention
They can play a role in metabolic disorders and can break down tough plant fibers: β-glycosidases are enzymes that play many roles in nature. Fredj Ben Bdira changed these enzymes in order to enhance the production of clean energy and to improve the treatment of patients with metabolic diseases.
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Madouc Bergers made her own enzyme inhibitors for her bachelor’s thesis
For her bachelor’s thesis, Molecular Science and Technology student Madouc Bergers synthesized her own molecule that can inhibit the breakdown of sugars. Although most students do not even manage to make one building block, Madouc made three. Partly because of this, she has been nominated for the Science…
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Scientists discover how to improve the production of antibiotics and enzymes in soil bacteria
A team of researchers at the Institute of Biology Leiden, in collaboration with scientists from Utrecht University, has discovered a novel approach to improve the production of antibiotics and enzymes in the soil bacteria Streptomycetes.
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Marcellus Ubbink
Wiskunde en Natuurwetenschappen
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Research shows protein movement is important
Researchers led by Professor of Chemistry Marcellus Ubbink have recently published a study in Proceedings of the National Academy of Sciences (PNAS) about the dynamics of an important redox enzyme. This work was accomplished thanks to an NWO VICI subsidy granted to Professor Ubbink.
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Unraveling the mechanism of multicopper oxidases: from ensemble to single molecule
Promotores: Prof.dr. G.W. Canters, Prof.dr. T.J. Aartsma
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Arthur Ram
Wiskunde en Natuurwetenschappen
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Paradigm-shifting theory highlights the importance of substrate flexibility in enzymatic reactions
Leiden chemists have proposed a new model for enzymatic reactions, in which the flexibility of the substrate is much more important than previously thought. Their results are paradigm-shifting and could have major implications for drug research and enzyme engineering. Publication in Angewandte Chemi…
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'Q-wires': Synthesis, electrochemical properties and their application in electro-enzymology
An objective of this research was to achieve direct, well-defined and non-rate-limiting electron transfer between respiratory enzymes and the electrode surface by means of 'Q-wires'.
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Activity-based protein profiling of glucosidases, fucosidases and glucuronidases
Promotores: H.S. Overkleeft, G.A. van der Marel, Co-promotor: B.I. Florea
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The relation between dynamics and activity of phospholipase A/acyltransferase homologs
Phospholipase A/acyltransferase 3 (PLAAT3) and PLAAT4 are enzymes involved in the synthesis of bioactive lipids. Despite sequential and structural similarities, the two enzymes differ in activity and specificity.
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Chemical biology of sphingolipids: fundamental studies and clinical applications
Overkleeft
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Cytochrome P450 3A-mediated first-pass and systemic drug metabolism in children
From descriptive to physiological models that can predict oral absorption and elimination of CYP3A substrates across the pediatric age range.
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Exploring chemical space in covalent and competitive glycosidase inhibitor design
Glycoside hydrolases (glycosidases/GHs) are widely abundant enzymes in all kingdoms of life and are important biocatalysts that catalyze the hydrolysis of glycosidic linkages in oligo/polysaccharides, glycoproteins and glycolipids with tremendous efficiency
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Microbial Sciences
In the research programme Microbial Sciences we perform state-of-the-art research in the field of biotechnology and microbial sciences.
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Research
Research at the Macromolecular Biochemistry group is comprised of the following research themes:
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Teaching
Research projects for students of the University of Leiden and other Dutch Universities are often available at the MacBio group. The research lines are also described in the Research section. For details of specific projects contact the supervisor (Ubbink, Dame, Boyle, Jeuken or Wentink).
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Efficient targeting of the Trichoderma genome for industrial protein engineering
The research is aimed at development of an efficient gene targeting method that allows controlled integration of DNA at a preselected site in the Trichoderma genome.
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Biomimetic models of [NiFe] hydrogenase for electrocatalytic hydrogen evolution
The growing demand of energy indicates that global energy resources in the form of fossil fuels will not be sufficient in the future. In order to solve potential future energy problems development of a sustainable hydrogen economy is highly desirable.
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Visualization of Vitamin A Metabolism
Vitamin A or retinol is essential in embryonic development, the visual cycle and the immune system.
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Applications of Natural Deep Eutectic Solvents to Extraction and Preservation of Biomolecules
The recently introduced nature-originated deep eutectic solvents, so-called natural deep eutectic solvents (NADES) are considered as truly green solvents, which composed of natural ingredients found abundantly in organisms.
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On Terminal Alkynes That Can React with Active-Site Cysteine Nucleophiles in Proteases
Active-site directed probes are powerful in studies of enzymatic function. We report an active-site directed probe based on a warhead so far considered unreactive.
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Chemical biology of glucosylceramide metabolism: fundamental studies and clinical applications for Gaucher disease
How can we develop new chemical biology tools and approaches to understand and interfere with glucosylceramide metabolism in relation to Gaucher disease?
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Bacteria that degrade plastic: Unilever Research Prize for master’s student Jo-Anne Verschoor
Jo-Anne Verschoor wins the Unilever Research Prize 2020. The master's student in Biology developed a method to test whether bacteria are capable of degrading plastic. Verschoor: 'Using specific enzymes from these bacteria, we may be able to efficiently recycle plastic in the future.
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The synthesis of chemical tools for studying sphingolipid metabolism
Sphingolipids are important membrane compounds with a variety of functions. In mammalian cells, different enzymes are involved in the metabolism of sphingolipids, but interruption of this metabolism process leads to different diseases.
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Cyclophellitol and its derivatives: synthesis and application as beta-glycosidase inhibitors
Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel
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Direct and two-step activity-based profiling of proteases and glycosidases
Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel
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Illuminating N-acylethanolamine biosynthesis with new chemical tools
In this thesis, the discovery and optimization is described of chemical tools to study the N-acylethanolamine (NAE) biosynthetic pathway.