Promotie

The molecular mechanisms that instigate a healthy cell to become malignant are fueled by (epi)genetic alterations in so-called driver genes.

Increasing the efficiency of gene targeting (GT) as a genome editing tool in plants has been an important goal in plant biotechnology.

Drug development is a time- and resource-consuming process that starts with the discovery and validation of a (protein) target that contributes to pathogenesis or disease progression.

In this thesis, we used genetically engineered mouse models to identify genes and pathways that are involved in ILC formation and in the development of resistance to FGFR-targeted therapy.

The RNA guided endonuclease based on the CRISPR/Cas system of Streptococcus pyogenus is a potent new tool for genome engineering in plants.

The human body consists of hundreds, perhaps thousands of different types of cells, each with different morphologies and functions, despite having the same genome.

Hoe beinvloed de structuur van één enkel gen de activiteit van genen die gevoelig zijn voor hormonen?

To improve cancer treatments, personalized medicine approaches have aimed to identify exactly which mutations are driving tumor development in a given patient and specifically target these mutations using precision therapies.

The objective of this study was to investigate the expression and function of GRHL2 in different breast cancer subtypes.

Promotores: T.H.W. Bäck, A. Plaat, Co-promotor: F.J. Verbeek

Promotie

Aspergillus niger is an important industrial producer of organic acids and enzymes producing large amounts of spent fungal biomass.

Promotor: Prof.dr. G.P. van Wezel

In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.

Streptomyces bacteria are a valuable source of natural products, many of which are used in the clinic or in biotechnology.

Nucleoid associated proteins maintain the architecture of the bacterial chromosome and regulate gene expression, hinting that their role as transcription factors may involve local three-dimensional chromosome re-modelling.

This thesis describes the functional analysis of the Arabidopsis AHL15 gene.

Promotor: P.J.J. Hooykaas, Co-Promotores: P.B.F. Ouwerkerk, M. Wang

Fungal food spoilage often starts with a contamination with spores. Experimental data strongly indicate the existence of subpopulations of spores with different levels of resistance to preservation methods.

In this Ph.D. thesis, we characterized two novel upstream regulators of JUB1, HB40, and HB22, in Arabidopsis thaliana. In Chapter 2, we described functions of HB40 in modulating growth and development via the regulation of JUB1 and GA inactivation genes GA2OXs.

Promotores: A.H. Meijer, H.P. Spaink

DNA carries various forms of information. Out of these forms of information the most well-known is classical genetic information.

My PhD project studies how the temporally sequential Hox gene expression is regulated during head-tail patterning of the frog embryo.

Promotor: P. J. J. Hooykaas Co-promotor: B. S de Pater

Plant architecture has distinct forms in different plant species, but also within a species the finalarchitecture of a plant is determined by its gradual development and changes therein induced by environmental conditions during the plant’s life cycle.

Drug induced organ toxicity is the main problem of the drug development and drug usage in the clinic. The liver and kidneys are the most sensitive organs towards drug induced toxicity.

The experiments described in this thesis employ local lentiviral knockdowns in brain areas of female zebra finches followed by behavioural assays consisting of preference and Go/Nogo tasks.

Plant-microbe interaction resulted in different physio/chemical responses by host plant and interacting rhizobacteria.

Salicylic acid (SA) is a plant hormone, which is involved in the defense responses to pathogens and to abiotic stress, and in the regulation of plant growth and development.

Climate change is a challenge for both current and future generations. New biological resources have to be developed in order to meet the demand for energy as well as the demand for food.

Promotie

The work described in this dissertation contributes to a better mechanistic understanding of nanoparticles in vivo. To achieve that goal, we used the zebrafish as a highly predictive pre-screening model of nanoparticles.

The clinical potential of applying synthetic lethality to cancer treatment is famously demonstrated by the BRCA1/PARP1 paradigm: a tumor specific defect in BRCA1 – a component of the DNA double-strand break (DSB) repair pathway homologous recombination (HR) – results in a remarkable sensitivity to PARP1…

To gain more insight into the evolutionary development of orchid flowers and fruits, the orchid species Erycina pusilla was studied. The evolutionary origin of the median petaloid sepal, the callus on the labellum, and the stelidia was studied.

Pectin is a plant cell wall polysaccharide made of mainly D-galacturonic acid (GA) subunits. The potency of the filamentous fungus Aspergillus niger to naturally secrete high amounts of pectinases to degrade pectin has been utilized for the industrial production of pectinases.

Soil is a home for an unbelievable diversity and abundance of microbial life that is essential for supporting life on our planet.

Promotores: Prof.dr. M.K. Richardson, Prof.dr. H.P. Spaink. Co-promotor: C.P. Bagowski

Promotors: Prof.dr. P.J. Punt, Prof.dr. C.A.M.J.J. van den Hondel

The type-IV secretion system (T4SS) is a machinery able to transfer DNA and proteins between bacteria and in certain cases also to eukaryotic cells.

Promotor: G.P. van Wezel, Co-promotor: Y.H. Choi

Promotie

Bij de specialisatie Bioinformatica aan de Universiteit Leiden leer je informatie uit de biologie structureren, analyseren en herkennen. Je behandelt zowel abstracte wiskundige vraagstukken als ook praktisch georiënteerde zaken zoals het beheersen en ontwerpen van computerprogramma’s.

Wiskunde en Natuurwetenschappen, Leiden Academic Centre for Drug Research (LACDR)

Promotor: H.P. Spaink, Co-Promotor: A.H. Meijer

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Promotie