PhD Defence

Kieltyka

In order to gain a better insight into the basis of the rapid jasmonic acid (JA)-mediated stress response, an integrated approach using a targeted high-resolution mass spectrometry-based (HRMS) platform, was conducted to monitor the metabolism of JA using the model system of cell suspension cultures…

Phosphorylation affects all four major biomolecules – proteins, lipids, carbohydrates and nucleic acids – and plays a pivotal role in the most fundamental cellular functions.

Evolution acts via mutations in amino acid sequences. Substitution of essential amino acids leads to a nonfunctional protein. l.

Antibiotic resistance, caused by widespread use of antibiotics, leads to bacterial infections that are difficult, if not impossible, to treat and is a major worldwide health concern.

Promotors: Prof.dr. E. Bouwman, Prof.dr. E. Drent

Het onderzoek binnen de Faculteit der Wiskunde en Natuurwetenschappen omvat de disciplines wiskunde, informatica, sterrenkunde, natuurkunde, scheikunde, bio-farmaceutische wetenschappen, biologie en milieuwetenschappen.

This thesis describes the use of bioorthogonal proteins in immunological settings. It provides an introduction towards the field of protein modification, which was used throughout the thesis for the expression of proteins containing unnatural amino acids.

Nederlandse onderzoekers hebben een nieuwe ‘gereedschapskist’ ontwikkeld om DNA te labelen voor het bestuderen van chromosomen. Hun tool is geschikt voor onderzoek naar cellen in kweek, maar ook in levende organismen zoals zebravisembryo's. Het team heeft zijn bevindingen gepubliceerd in het tijdschrift…

Onderzoekers van de Universiteit Leiden en de Vrije Universiteit Amsterdam hebben bepaald hoe erfelijke informatie is georganiseerd in archaea, eencellige organismen die onder extreme omstandigheden kunnen overleven. De resultaten van het onderzoek staan in Nature Communications (27 december 2012) en…

Promotor: A.P. IJzerman

Science, Leiden Academic Centre for Drug Research (LACDR)

Promotor: M.T.M. Koper, Co-Promotor: L.B.F. Juurlink

De manier waarop genen zijn opgevouwen bepaalt hoe vaak ze tot uiting komen in ons lichaam. Leidse biofysici hebben nu als eerste deze structuur molecuul-voor-molecuul ontrafeld. Publicatie op 1 december in Nature’s Scientific Reports.

The soil ecosystem consists of the largest reservoir of biodiversity on Earth.

Astronomical observations of cold regions in the universe show a rich inventory of ices. Part of these ices may end up on planets like our own, but in that journey they will be exposed to considerable amounts of radiation.

This dissertation focused on computational methods based on first principles calculations using the Density Functional Theory (DFT) framework. Emphasis was laid on affordable methods that can provide a tradeoff between computational expense and accuracy.

Dissolving microneedles are a subgroup of microneedle types that completely dissolve within the skin. During this dissolving process the vaccine, stored in the needle matrix, is released into the skin.

This thesis describes the design and development of subunit‐selective inhibitors of particular catalytically active subunits of human constitutive proteasomes and immunoproteasomes.

Promotor: Prof.dr. R. Verpoorte, Co-Promotores: N.R. Mustafa, A.E. Schulte

In this Ph.D. thesis, we characterized two novel upstream regulators of JUB1, HB40, and HB22, in Arabidopsis thaliana. In Chapter 2, we described functions of HB40 in modulating growth and development via the regulation of JUB1 and GA inactivation genes GA2OXs.

The research described in this thesis shows that hypercholesterolemia, a well-established risk factor for atherosclerosis, can impact skin lipid pool and barrier function already at young age. In the field of atherosclerosis, we showed that the small peptide Lyp-1 can be used as a targeting molecule…

Promotor: Prof.dr. H.V.J. Linnartz, Co-Promotores: S. Ioppolo, H.M. Cuppen

Barrier function is the natural role of the skin. The lipid matrix present in the outermost layer of the skin, the stratum corneum is important for this function.

Pectin is a plant cell wall polysaccharide made of mainly D-galacturonic acid (GA) subunits. The potency of the filamentous fungus Aspergillus niger to naturally secrete high amounts of pectinases to degrade pectin has been utilized for the industrial production of pectinases.

The recently introduced nature-originated deep eutectic solvents, so-called natural deep eutectic solvents (NADES) are considered as truly green solvents, which composed of natural ingredients found abundantly in organisms.

Promotor: Prof.dr. J.G.E.M. Fraaije, Co-promotor: A. Kros

Aspergillus niger is an important industrial producer of organic acids and enzymes producing large amounts of spent fungal biomass.

In this thesis an activity-based probe was discovered that could visualize the activity of PLAATs. With an optimized gel-based ABPP assay in hand, screening of a compound library led to the discovery of alpha-ketoamides as a hit for PLAAT3.

The focus throughout this thesis will be on gathering fundamental studies of the detailed structure and composition of the electrode/electrolyte interface effect on the rate and mechanism of key electrocatalytic reactions.

Natural vanilla flavor is one of the most important in the world. However, the cost of this flavor is expensive. Production of this flavor by alternative methods, could reduce the cost.

PhD Defence

PhD Defence

Naast de 2 TOP grants heeft het LIC ook nog 4 ECHO’s binnengehaald voor dr. Francesco Buda, professor Gijs van der Marel met Jeroen Codée, dr. Dmitri Filippov en professor Gilles van Wezel.

The aim of this thesis is to explore the use of Bioorthogonal Antigens to study the cross-presentation pathway.

The thesis focuses on synthesis strategies in oligosaccharide campaigns, and the influence of protecting groups.

This thesis describes the antimicrobial discovery strategy developed in our group, the den Hertog Group at the Hubrecht Institute. It includes a cultivation-based screening approach for novel antimicrobial agents from the source of fungi, and a bacterial time-lapse imaging approach for antimicrobial…

Lecture

PhD Defence

PhD Defence

Bicarbonate buffer is largely found in nature due to its ability to regulate pH variations around neutral values. As the pH changes, so does the speciation of the buffer.

Vitamin A or retinol is essential in embryonic development, the visual cycle and the immune system.

Promotores: W. Jiskoot, F. Ossendorp

Promotor: H.V.J. Linnartz

Fungal food spoilage often starts with a contamination with spores. Experimental data strongly indicate the existence of subpopulations of spores with different levels of resistance to preservation methods.

Promotores: Prof.dr. C.A.M.J.J. van den Hondel, Prof.dr. V. Meyer (Technische Universität Berlin)

With the energy transition toward a renewable energy supply and a CO2-neutral economy, electrification of the energy system is rising in importance, which leads to the challenge of long-term storage of renewable electricity.

Decentralized plug and play systems for energy production are the future picture of our society. Artificial photosynthetic systems are used for this purpose.