In this Ph.D. thesis, we characterized two novel upstream regulators of JUB1, HB40, and HB22, in Arabidopsis thaliana. In Chapter 2, we described functions of HB40 in modulating growth and development via the regulation of JUB1 and GA inactivation genes GA2OXs.

In order to gain a better insight into the basis of the rapid jasmonic acid (JA)-mediated stress response, an integrated approach using a targeted high-resolution mass spectrometry-based (HRMS) platform, was conducted to monitor the metabolism of JA using the model system of cell suspension cultures…

Evolution acts via mutations in amino acid sequences. Substitution of essential amino acids leads to a nonfunctional protein. l.

Antibiotic resistance, caused by widespread use of antibiotics, leads to bacterial infections that are difficult, if not impossible, to treat and is a major worldwide health concern.

Kieltyka

PhD Defence

Promotors: Prof.dr. E. Bouwman, Prof.dr. E. Drent

This thesis describes the use of bioorthogonal proteins in immunological settings. It provides an introduction towards the field of protein modification, which was used throughout the thesis for the expression of proteins containing unnatural amino acids.

Promotor: A.P. IJzerman

Promotor: M.T.M. Koper, Co-Promotor: L.B.F. Juurlink

The soil ecosystem consists of the largest reservoir of biodiversity on Earth.

Astronomical observations of cold regions in the universe show a rich inventory of ices. Part of these ices may end up on planets like our own, but in that journey they will be exposed to considerable amounts of radiation.

This dissertation focused on computational methods based on first principles calculations using the Density Functional Theory (DFT) framework. Emphasis was laid on affordable methods that can provide a tradeoff between computational expense and accuracy.

Na de Academische Pabo kun je direct aan het werk in het basisonderwijs. Maar mocht je toe zijn aan meer verdieping, dan kun je ook nog een masteropleiding volgen.

Dissolving microneedles are a subgroup of microneedle types that completely dissolve within the skin. During this dissolving process the vaccine, stored in the needle matrix, is released into the skin.

This thesis describes the design and development of subunit‐selective inhibitors of particular catalytically active subunits of human constitutive proteasomes and immunoproteasomes.

Promotor: Prof.dr. R. Verpoorte, Co-Promotores: N.R. Mustafa, A.E. Schulte

The research described in this thesis shows that hypercholesterolemia, a well-established risk factor for atherosclerosis, can impact skin lipid pool and barrier function already at young age. In the field of atherosclerosis, we showed that the small peptide Lyp-1 can be used as a targeting molecule…

Promotor: Prof.dr. H.V.J. Linnartz, Co-Promotores: S. Ioppolo, H.M. Cuppen

Barrier function is the natural role of the skin. The lipid matrix present in the outermost layer of the skin, the stratum corneum is important for this function.

Pectin is a plant cell wall polysaccharide made of mainly D-galacturonic acid (GA) subunits. The potency of the filamentous fungus Aspergillus niger to naturally secrete high amounts of pectinases to degrade pectin has been utilized for the industrial production of pectinases.

The recently introduced nature-originated deep eutectic solvents, so-called natural deep eutectic solvents (NADES) are considered as truly green solvents, which composed of natural ingredients found abundantly in organisms.

Promotor: Prof.dr. J.G.E.M. Fraaije, Co-promotor: A. Kros

Aspergillus niger is an important industrial producer of organic acids and enzymes producing large amounts of spent fungal biomass.

Ze stond aan de wieg van de Honours Academy en heeft honoursonderwijs binnen de Universiteit Leiden zien groeien. Binnenkort neemt Chris de Kruif na vijf jaar afscheid van de Honours Academy. “Als onderwijsdirecteur had ik contacten met alle faculteiten, die collega's ga ik zeker missen.”

Wil je jezelf onderdompelen in een nieuwe cultuur, je horizon verleggen en levenslange vrienden maken? Kom dan op vrijdagmiddag 14 oktober naar het Study Abroad Festival.

Phosphorylation affects all four major biomolecules – proteins, lipids, carbohydrates and nucleic acids – and plays a pivotal role in the most fundamental cellular functions.

In this thesis an activity-based probe was discovered that could visualize the activity of PLAATs. With an optimized gel-based ABPP assay in hand, screening of a compound library led to the discovery of alpha-ketoamides as a hit for PLAAT3.

The focus throughout this thesis will be on gathering fundamental studies of the detailed structure and composition of the electrode/electrolyte interface effect on the rate and mechanism of key electrocatalytic reactions.

Natural vanilla flavor is one of the most important in the world. However, the cost of this flavor is expensive. Production of this flavor by alternative methods, could reduce the cost.

PhD Defence

PhD Defence

Naast de 2 TOP grants heeft het LIC ook nog 4 ECHO’s binnengehaald voor dr. Francesco Buda, professor Gijs van der Marel met Jeroen Codée, dr. Dmitri Filippov en professor Gilles van Wezel.

The aim of this thesis is to explore the use of Bioorthogonal Antigens to study the cross-presentation pathway.

The thesis focuses on synthesis strategies in oligosaccharide campaigns, and the influence of protecting groups.

This thesis describes the antimicrobial discovery strategy developed in our group, the den Hertog Group at the Hubrecht Institute. It includes a cultivation-based screening approach for novel antimicrobial agents from the source of fungi, and a bacterial time-lapse imaging approach for antimicrobial…

Het ene bedrijf staat door de coronapandemie aan de rand van de afgrond, het andere maakt juist nu veel winst. Politici denken daarom na over een coronataks, waarbij de winnaars van deze situatie kunnen dan bjdragen aan het herstel van de verliezers.

We moeten ervan uitgaan dat dieren gevoelens kunnen hebben. Dat moet vanuit ethisch oogpunt dan ook leidend zijn in onze omgang met dieren, zo betogen onderzoekers van de Universiteit Leiden en de Universiteit Utrecht donderdag 10 maart in een opinieartikel in het wetenschappelijke tijdschrift Affective…

Wil jij je mening laten horen over de opleiding, de faculteit en de universiteit en lijkt het je leuk om met een bestuurder in gesprek te gaan? Geef je op en misschien ben jij een van de studenten die wordt geselecteerd voor de lunchbijeenkomst op 5 juli 2017 met assessor Aurelie van ’t Slot en vice-decaan…

In het nieuwe online diversiteit-en-inclusiedossier zijn alle facultaire initiatieven op dit gebied gebundeld. Maar hoe staat het er verder voor met diversiteit en inclusie bij Geesteswetenschappen? Een gesprek met Aurelie van ’t Slot, beleidsmedewerker Internationalisering, Diversiteit & Inclusie.

Op 17 november is minister Koenders van Buitenlandse Zaken te gast bij de Faculteit Governance and Global Affairs van de Universiteit Leiden om over de trans-Atlantische band en andere zaken te spreken.

PhD Defence

PhD Defence

Bicarbonate buffer is largely found in nature due to its ability to regulate pH variations around neutral values. As the pH changes, so does the speciation of the buffer.

Vitamin A or retinol is essential in embryonic development, the visual cycle and the immune system.

Promotores: W. Jiskoot, F. Ossendorp

Promotor: H.V.J. Linnartz

‘Wij zijn één universiteit in twee steden met drie campussen’, aldus vice-rector Hester Bijl. Tijdens de decanenbijeenkomst op donderdag 27 september stond de Haagse vestiging centraal. Verschillende decanen van middelbare scholen uit Den Haag en omstreken, kwamen bijeen om van gedachten te wisselen…