Bacterial chemosensory arrays are protein assemblies that are the key structural and functional component for motile bacteria to sense their internal or environmental chemical signals.

Cryogenic electron microscopy (cryo-EM) is a powerful technique used to visualize the inside of cells and to study specific protein complexes. Within this thesis, I describe the use of various cryo-EM techniques to gain insight into the structural changes of the human pathogen, Vibrio cholerae, as it…

The structure of bacterial cells provides crucial clues about their interaction with their host. What are the key structural features of a bacterial cell that determine pathogenicity? What roles do these structures play in the life cycle, and how do they change during the infection process?

Can we determine the 3D structure of a chemotaxis core complex using single-particle reconstructions via cryo-EM?

Electron microscopy has become an extremely important techniquein a wide variety of elds.

X-Ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR) and Cryo-electron microscopy (Cryo-EM) are the three main methods for solving structures of macromolecules.

The aim of this facility is to accelerate biomedical research by providing access to advanced light and electron microscopy and by sharing expertise about sample preparation, imaging modalities, data storage and image analysis.

The Microscopy Unit houses, maintains and coordinates most of the microscopy equipment of the IBL. The available equipment ranges from conventional light and fluorescence microscopes, to confocal laser scanning and electron microscopes. In addition, infrastructure is available for histology, including…

Promotor: Prof.dr. J.M. van Ruitenbeek, Prof.dr. R.M. Tromp

The dissertation describes TEM experiments on heterogeneous catalysts.

n this PhD thesis, the recombination of different atomic lattices in stacked 2D materials such as twisted bilayer graphene is studied. Using the different possibilities of Low-Energy Electron Microscopy (LEEM), the domain forming between the two atomic layers with small differences is studied.

In condensed matter systems electron-electron interactions, negligible in everyday metals, can dramatically alter the electronic behavior of the system. Examples of such altered behavior include high-temperature superconductivity and modulation of the electron density.

Promotores: Prof.dr. H.P. Spaink & Prof.dr. P.C.W. Hogendoorn, Co-promotor: Dr. M.J.M. Schaaf

Promotor: Prof.dr. J.P. Abrahams, Prof.dr. M. van Heel

This thesis described the development of novel scanning tunneling microscopy techniques to investigate strongly correlated electronic states in quantum matter.

Promotor: J.P. Abrahams, Co-promotor: T. Grüne

Bacteria have the ability to alter their morphology in order to adapt to changing environments.

Promotor: T. Schmidt

This thesis describes a study from both a theoreticaL and an experimental point of view.

Materials with strongly correlated electrons show some of the most mysterious and exotic phases of quantum matter, such as unconventional superconductivity, quantum criticality and strange metal phase.

The Netherlands Organisation for Scientific Research (NWO) has made a subsidy of 17 million euros available to further develop a Netherlands network for electron microscopy (NEMI). The network comprises five UMCs and eight universities, with Utrecht in the coordinating role. From Leiden, the Institute…

Single-Molecule Microscopy (SMM) techniques constitute a group of powerful imaging tools that enable researchers to study the dynamic behavior of individual molecules.

Chinese and Leiden scientists came together in Leiden to study the intricacies of modern visual techniques.

This is a yearly event where we move in one week from the physics of light through the composition of the microscope, fluorescence microscopy and end up with some image analysis All the lectures are backed by practicals to really put your obtained knowledge into practice

How do we experience electronic music? How does electronic music operate on perceptual, cognitive and affective levels? What are the common concepts activated in the listener’s mind when listening to electronic music? Why and how are these concepts activated?

From The Cognitive Continuum of Electronic Music:

With the powerful electron microscopes at NeCEN we study abnormalities in cells of blood vessels in cardiovascular disease, the interaction between pathogens and hosts in infectious diseases, molecular processes that cause cancer and the interaction between drugs and target proteins. Drug development…

What is the Electronics Department?

In surface science there is great effort to move from studying simple, flat model surfaces in vacuum to investigating more complex model catalysts in gas environments (in situ). This thesis gives three examples of such studies using microscopy and spectroscopy.

Promotor: T.H. Oosterkamp

In this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms.

In this thesis, we describe the latest advances in SQUID-detected Magnetic Resonance Force Microscopy (MRFM).

Single-molecule spectroscopy has become a powerful method for using organic fluorescent molecules in numerous applications.

In this thesis we use Josephson and noise scanning tunneling microscopy for the study of conventional, unconventional (iron-based) and disordered superconductors. On the one hand, Josephson scanning tunneling microscopy allows us to directly visualize the superfluid density with high spatial resolut…

In this thesis the combinatorial use of bioorthogonal labelling and Electron Microscopy (EM)-based imaging techniques is explored to enable observations of specific molecular targets in their ultrastructural context within the cell.

In this thesis, we show how MRFM can usefully contribute to the field of condensed-matter.

Intrigued by the way cells autonomously regulate their fate, we strive to understand and visualize cellular processes as the basis of Cell Signaling.

We explore the possibilities to combine magnetic resonance techniques with atomic force microscopy together in a single microscope: the MRI-AFM, also called Magnetic Resonance Force Microscopy (MRFM).

In order to gain insight into the structure and function of the molecular complexes involved in chemotaxis, we use electron cryotomography (ECT). This technique allows us to directly study microbes in their native state at resolutions capable of visualizing individual proteins.

Bacteria are everywhere. They are the most abundant organisms on earth and impact all aspects of our lives. They determine our health and shape our environment. Ariane Briegel, professor of Ultrastructural Biology, freezes bacteria super fast to gain a true-to-nature image of the internal and external…

Promotores: Prof.dr. C.U. Keller, Prof.dr. H.C. Gerritsen

Atoms and molecules are the basic units of matter. If we keep dividing a bar of gold or a glass of water into smaller parts, at the end we are left with a single gold atom or a water molecule.

Physicist Daniël Geelen has developed a new microscope that uses low-energy electrons. Those are less harmful to biological and organic materials. Geelen defends his PhD thesis on May 31st.

Prof. Dr. Ir. Marin van Heel, Emeritus Professor at the Institute of Biology Leiden (NeCEN), is one of the winners of the “Wiley Prize in Biomedical Sciences 2017”. This annual award is presented to recognize contributions that have opened new fields of research or have advanced concepts in a particular…

The principal aim of this research is to explore the confrontation between voice and electronics. These are often seen as being radically different from one another and many characteristics seem to be in opposition between them. However, they also have characteristics in common and they can influence…

This thesis revolves around the electronic spectroscopy of molecules in the laboratory and the search for the carriers of the diffuse interstellar bands (DIBs).