The challenge of achieving fast quantification in metabolomics is the presence of severe matrix effects during the MS analysis of complex samples.

How can ecosystem services within river catchments be quantified in spatial and temporal explicit way?

In this project, we aim to obtain a quantitative understanding of the role of the immune system in tumor regression, of the role of tumor cell heterogeneity in cancer growth and of tumor cell migration properties.

Promotor: Prof.dr. T. Hankemeier, Co-promotor: T.H. Reijmers

Optical projection tomography (OPT) is a tomographic 3D imaging technique used for specimens in the millimetre scale.

Promotor: R.C.M. Pelger Co-Promotor: G. van der Pluijm

Epithelial-mesenchymal plasticity (EMP) and tumor cell migration play an important role in cancer progression, and an improved understanding of the mechanisms underlying these concepts is essential for developing new targeted approaches.

The work in this thesis describes the fundamental role of Lkb1 as a conductor of metabolism-related processes in zebrafish larvae.

Growth hormone profiles are pulsatile and highly variable between individuals, limiting the implementation of mathemathical models to quantify an individual's secretion.

The main topic of the book is the nature of inner aspect of the Verb Phrase, and the relation between the decompositional and the quantificational approaches to this problem.

Inactivating mutations in BRCA1 or BRCA2 genes predispose to several types of cancer.

Activity-based protein profiling (ABPP) is a functional proteomics technique for directly monitoring the expression of active enzymes in cell extracts and living cells. The technique relies on irreversible inhibitors equipped with reactive groups (warheads) that covalently attach to the active site…

Dendritic cells (DC) play a prominent role in the priming of CD8(+) T cells. Vaccination is a promising treatment to boost tumor-specific CD8(+) T cells which is crucially dependent on adequate delivery of the vaccine to DC.

PhD defence

Advanced mass spectrometry of glycosphingolipids takes the central stage in this thesis. Investigations focus on characterization of glycosphingolipid metabolism in health and disease with emphasis to the detection and accurate quantitation of known and so far unknown glycosphingolipids and closely…

Promotor: A.H. Meijer, Co-promotor: B.E. Snaar-Jagalska

From the 11th of May Ewa Snaar-Jagalska has been appointed Professor in the field of cellular tumor biology within the Faculty of Science at the Institute of Biology Leiden. Her research focuses on the cellular tumor biology and human cancer modeling in zebrafish

In this thesis, we used genetically engineered mouse models and a variety of cell-culture based assays to identify genes and pathways that are involved in the development and treatment of invasive lobular carcinoma (ILC).

The potential of bioneedles to deliver influenza vaccines was investigated.

CONCLUSION: This study demonstrates that human macrophages can be induced to exert direct anti-tumor activity against osteosarcoma cells. Our observation that the induction of macrophage anti-tumor activity by L-MTP-PE required IFN-gamma may be of relevance for the optimization of L-MTP-PE therapy in…

Inaugural lecture

Invasive lobular carcinoma (ILC) is the second most common type of breast cancer.

The zebrafish is a promising vertebrate model organism in early drug discovery and development.

Research project 'QUAD'

Promotor: Prof.dr. B. van de Water, Co-promotores: J.W. van der Laan, C.L.E. Siezen

The main aim of this thesis was to unravel the signaling and regulatory networks that drive tumor cell migration during breast cancer metastasis.

Bispecific antibodies with one Fab arm binding to effector cells, such as T-cells and NK-cells, while the other Fab arm recognizing a tumor associated antigen (TAA) can be used to redirect these effector cells for tumor specific killing. Using bispecific antibodies to treat hematological cancers is…

PhD defence

Anthropogenic noise has been shown to affect marine animals in various ways, this may have fitness consequences at individual and population level. This thesis aims to increase insight into the quantification of sound-induced behavioural responses that are relevant to fitness, and into factors that…

This dissertation provides a novel perspective on the interaction between quantifier scope and ellipsis. It presents a detailed investigation of the scopal interaction between English negative indefinites, modals, and quantified phrases in ellipsis. One of the crucial observations is that a negative…

The potential of liposomal cancer vaccines

Cytotoxic T-cells (CTLs) are involved in the clearance of viruses and killing of tumor cells.

Promotores: J. Jonkers, K.E. de Visser

The molecular mechanisms that instigate a healthy cell to become malignant are fueled by (epi)genetic alterations in so-called driver genes.

Exploiting metabolic vulnerabilities to identify anticancer compounds in zebrafish synthetic lethality screens.

How can zebrafish research help to understand and fight human cancer?

PhD defence

PhD defence

PhD defence

PhD defence

PhD defence

Promotor: A. Doelman, Co-promotores: P.J.A. van Heijster; A. Zagaris

Projects within the theme ‘Computational Biology’ aim to employ mathematical or computational dynamical modelling approaches to discover novel concepts that are important for drug development.

We focus on a better understanding of the mechanisms of cancer drug resistance and metastasis.

PhD defence

Novel methods for understanding key aspects that are essential to the future of Bayesian inference for high- or infinite-dimensional models and data.