451 search results for “skin lipid organization” in the Public website
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Human skin equivalents: understanding the lipid biosynthesis in the skin
There is considerable need for improved skin models to develop safe and efficient drugs for skin delivery as well as for cosmetic and chemical product testing on the skin.
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Modulation of Stratum Corneum Lipid Composition and Organization of Human Skin Equivalents by Specific Medium Supplements
Our in-house human skin equivalents contain all stratum corneum (SC) barrier lipid classes, but have a reduced level of free fatty acids (FAs), of which a part is mono-unsaturated. These differences lead to an altered SC lipid organization and thereby a reduced barrier function compared to human skin.…
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Intercellular Skin Barrier Lipid Composition and Organization in Netherton Syndrome Patients
Netherton Syndrome (NTS) is a rare genetic skin disease caused by mutations in the serine protease inhibitor Kazal-type 5 gene, which encodes the lympho-epithelial Kazal-type-related inhibitor. NTS patients have a profound impaired skin barrier function. Because SC lipids play a crucial role in the…
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Phase behavior of skin lipid mixtures: the effect of cholesterol on lipid organization
The lipid matrix in the stratum corneum (SC), the upper layer of the skin, plays a critical role in the skin barrier. The matrix consists of ceramides (CERs), cholesterol (CHOL) and free fatty acids (FFAs).
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Lipids as therapeutic targets for barrier repair in skin diseases
The skin is our natural barrier and lipids are a key part of this barrier. In the outer skin layer, the stratum corneum (SC), lipids form a densely organized structure dependent on the composition of these lipids.
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Understanding the physical properties that affect the lipid organization in the skin barrier
It is the aim of this project to better understand how the physical properties of lipids influences the structure and overall barrier function of the skin.
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In vitro model systems for studying the impact of organic chemicals on the skin barrier lipids
This paper describes two synthetic lipid models designed to replace human stratum corneum (SC) in studies of the impact of volatile organic chemicals on the molecular organization of the skin barrier lipids.
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distribution affects permeability barrier function of stratum corneum lipid model membranes
Source: International Journal of Cosmetic Science, Volume 37, Number 1, pp. 158-158 (2015) ISBN: 0142-5463
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An ex vivo human skin model for studying skin barrier repair
In the studies described in this study, we introduce a novel ex vivo human skin barrier repair model. To develop this, we removed the upper layer of the skin, the stratum corneum (SC) by a reproducible cyanoacrylate stripping technique.
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The important role of stratum corneum lipids for the cutaneous barrier function
The skin protects the body from unwanted influences from the environment as well as excessive water loss. The barrier function of the skin is located in the stratum corneum (SC).
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Cutaneous barrier dysfunction in atopic eczema: the role of stratum corneum lipids
Source: International Journal of Cosmetic Science, Volume 37, Number 1, pp. 148-148 (2015) ISBN: 0142-5463
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Lipid to protein ratio plays an important role in the skin barrier function of atopic eczema patients
The barrier function of the skin is primarily provided by the stratum corneum (SC), the outermost layer of the skin. Skin barrier impairment is thought to be a primary factor in the pathogenesis of atopic eczema (AE).
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The role of lipids in the barrier function of the skin
The outermost layer of the skin, the stratum corneum (SC), is responsible for the skin barrier function, protecting the body from pathogens, chemicals and other unwanted substances from the external environment. The SC lipid matrix provides the only continuous pathway through the SC and is considered…
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the potentials of nurture: 2(nd) and 3(rd) generation explant human skin equivalents
BACKGROUND: Explant human skin equivalents (Ex-HSEs) can be generated by placing a 4mm skin biopsy onto a dermal equivalent. The keratinocytes migrate from the biopsy onto the dermal equivalent, differentiate and form the epidermis of 1(st) generation Ex-HSEs. This is especially suitable for the expansion…
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A novel formulation for skin barrier repair : from ex vivo assessment towards clinical studies
The stratum corneum is the outermost skin layer and consists of dead cells embedded in a lipid matrix.
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ceramide chain length distribution on the barrier properties of the skin lipid membranes
The skin barrier function is provided by the stratum corneum (SC). The lipids in the SC are composed of three lipid classes: ceramides (CERs), cholesterol (CHOL) and free fatty acids (FFAs) which form two crystalline lamellar structures. In the present study, we investigate the effect of CER chain length…
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A Model Membrane Approach to Elucidate the Molecular Organization in the Skin Barrier
Promotor: J. A. Bouwstra
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Lipid mediated colloidal interactions
The lipid membrane is a basic structural component of all living cells. Embedded in this nanometer-thin barrier, membrane proteins shape the membrane and at the same time respond to the shape of the membrane.
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Impaired barrier of inflammatory skin diseases
Focus in skin research
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Superstructures of lipids and graphene
This thesis systematically analyzes the physical-chemistry of lipid-graphene interactions with the major objective of reconciliating the variety of results reported in the literature.
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Human skin equivalents for atopic dermatitis: investigating the role of filaggrin in the skin barrier
Promotor: Prof.dr. J.A. Bouwstra, Co-promotor: Dr. A. El Ghalbzouri
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Skin Barrier and Vaccination
The Skin Barrier and Vaccination group is led by Prof. Joke Bouwstra. One of the key functions of the skin is its barrier function, which is located in the uppermost layer of the skin, the stratum corneum. The stratum corneum consists of enucleated dead cells (corneocytes) embedded in a lipid matrix.…
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Development of human skin equivalents to unravel the impaired skin barrier in atopic dermatitis skin
Promotor: J.A. Bouwstra Co-promotor: A. El Ghalbzouri
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Skin Lipids: Localization of Ceramide and Fatty Acid in the Unit Cell of the Long Periodicity Phase
The lipid matrix of the skin's stratum corneum plays a key role in the barrier function, which protects the body from desiccation. The lipids that make up this matrix consist of ceramides, cholesterol, and free fatty acids, and can form two coexisting crystalline lamellar phases: the long periodicity…
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LC/MS-platform for analysis of all major stratum corneum lipids, and the profiling of skin substitutes
Ceramides (CERs), cholesterol, and free fatty acids (FFAs) are the main lipid classes in human stratum corneum (SC, outermost skin layer), but no studies report on the detailed analysis of these classes in a single platform.
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Lipid to protein ratio plays an important role in the skin barrier function of atopic eczema patients
OBJECTIVES: To determine whether the lipid/protein ratio and the total dry SC mass per surface area are related with the skin barrier function of controls and AE patients.
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Human skin equivalent barrier optimization
The currently available in vitro generated human skin equivalents resemble the human skin in many aspects. However, some essential barrier characteristics do not fully mimic the native barrier. Consequently, the human skin equivalents cannot be used for screening of drugs for skin penetration.
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Lipid chain length reduction correlates with the skin barrier function in atopic eczema patients and inflammation plays a role in the altered
Source: Journal of Investigative Dermatology, Volume 135, pp. S59-S59 (2015)
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Reduced stratum corneum lipid chain length relates to an impaired skin barrier function in patients with atopic dermatitis
Source: British Journal of Dermatology, Volume 170, Number 6, pp. E18-E19 (2014) ISBN:0007-0963
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Human epidermal lipid biosynthesis in health and disease
How are the epidermal lipid pathways involved in health and disease.
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On the geometry of demixing: A study of lipid phase separation on curved surfaces
Like a mixture of oil and water, lipid membranes separate into two liquid phases.
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A novel biofilm for skin barrier repair of patients with atopic eczema
Atopic eczema is a skin disease with increasing incidence currently affecting 25% of the paediatric population. In atopic eczema the skin barrier function is reduced. This barrier is formed by the outermost layer of the skin called the stratum corneum (SC). The SC consists of terminally differentiated…
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The importance of free fatty acid chain length for the skin barrier function in atopic eczema patients
An important feature of atopic eczema (AE) is a decreased skin barrier function. The stratum corneum (SC) lipids - comprised of ceramides (CERs), free fatty acids (FFAs) and cholesterol - fulfill a predominant role in the skin barrier function.
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Lipid bilayers decorated with photosensitive ruthenium complexes
Promotor: E. Bouwman, Co-promotor: S. Bonnet
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Chemical tools to study lipid signaling
Synthesis and application of chemical biology tools to study immunomodulatory signaling lipids.
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Barrier properties of an N/TERT based human skin equivalent
Human skin equivalents (HSEs) can be a valuable tool to study aspects of human skin, including the skin barrier, or to perform chemical or toxicological screenings.
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Monounsaturated fatty acids reduce the barrier of stratum corneum lipid membranes by enhancing the formation of a hexagonal lateral packing
The effectiveness of the skin barrier underlies the outer layer of the skin: the stratum corneum (SC). However, in several skin diseases this barrier is impaired. In two inflammatory skin diseases, atopic eczema and Netherton syndrome, an increased level of monounsaturated fatty acids (MUFAs) has been…
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Coiled-coils on lipid membranes: a new perspective on membrane fusion
Promotor: J.G.E.M. Fraaije, Co-Promotor: A. Kros
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The interplay between lipids and the immune system in atherosclerosis
Cardiovascular diseases are among the most frequent causes of death in the world. The main underlying pathology of cardiovascular diseases is the development of atherosclerosis in the medium and large-sized arteries.
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Characterization and skin permeation of ketoprofen-loaded vesicular systems
PURPOSE: To determine the effect of elasticity on the skin permeation of ketoprofen from surfactant-based vesicular formulations and elastic liposomes.
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Rapid and profound rewiring of brain lipid signaling networks by acute diacylglycerol lipase inhibition
Diacylglycerol lipases (DAGLα and DAGLβ) convert diacylglycerol to the endocannabinoid 2-arachidonoylglycerol. Our understanding of DAGL function has been hindered by a lack of chemical probes that can perturb these enzymes in vivo.
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Membrane Fusion Mediated Intracellular Delivery of Lipid Bilayer Coated Mesoporous Silica Nanoparticles
Alexander Kros and co-workers describe how mesoporous silica nanoparticles (MSNs) are delivered into cells via membrane fusion thereby omitting endocytosis pathways. Fusion is induced by a pair of complementary coiled-coil lipopeptides inserted into the membrane of cells and in the bilayer of lipid-coated…
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Millions of people to help with new synthetic skin cream
A team of researchers has managed to recreate the most natural skin cream in existence. Using the DUBBLE beamline (Dutch Belgian Beamline) at the European Synchrotron Radiation produced on the basis of the information identified, has the same properties as vernix caseosa. This cream may have a therapeutic…
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PhD Thesis: Development of human skin equivalents to unravel the impaired skin barrier in atopic dermatitis skin
Recently, the PhD thesis of Lolu Danso Eweje appeared entitled ’Development of human skin equivalents to unravel the impaired skin barrier in atopic dermatitis skin’.
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Impact-Insertion Applicator Improves Reliability of Skin Penetration by Solid Microneedle Arrays
Source: The AAPS journal, Volume 16, Issue 4, pp. 681-684 (2014)
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The diverse roles of integrin α3β1 in cancer: lessons learned from skin and breast carcinogenesis
PhD Defence
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White Skin, Savage Masks: Specters of Native Americans and the Revisiting of History
How to related figures of savages to conceptions of history?
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Organization
Organization of the Leiden Institute of Physics.
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Research lines
Current research at the Skin Barrier and Vaccination group.
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Bio-organic Synthesis
The research in the Biosyn group is focused on the design, synthesis and function of the four major types of biomolecules: nucleic acids, carbohydrates, peptides and lipids and hybrid structures thereof. These biomolecules and their derivatives are used in drug discovery and chemical biology, to develop…