90 search results for “proteomics” in the Public website
- Center for Proteomics and Metabolomics
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Activity-based proteomics of the endocannabinoid system
This thesis describes the use of an activity-based proteomics method to study the endocannabinoid system.
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Innovative strategies to clinically characterize the human tear proteome
Transplantation of labial salivary glands to the eyelids for patients with dry eye appears to give excellent results clinically.
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PROTEOMINING: a novel proteomics-based pipeline for drug and enzyme discovery in filamentous actinomycetes
Can a new proteomics workflow be developed to link genes and gene clusters to bioactive molecules, identify novel compounds and enhance the production in the Streptomyces lividans enzyme?
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Bing Xie
Science
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Metabolic signatures in nutrition and health : short-term diet response, sexual dimorphism and hormone chronobiology
The power of personalized nutrition lies in being able to conduct clinical research on healthy people while capturing metabolic markers sensitive to the impact of environmental and metabolic stressors (e.g. diet, changing sex hormones and the menstrual cycle).
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Hermen Overkleeft
Science
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Discovery of FLT3 inhibitors for the treatment of acute myeloid leukemia
The disease acute myeloid leukemia (AML) is characterized by fast progression and low survival rates.
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Activity-based protein profiling of diacylglycerol lipases
Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt
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Visualization of Vitamin A Metabolism
Vitamin A or retinol is essential in embryonic development, the visual cycle and the immune system.
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Chao Du
Science
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Advancing Zebrafish Models in Proteomics
PhD Defence
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Exploring the proteome by CE-ESI-MS
PhD Defence
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Immunoproteomic Profiling of Bordetella pertussis Outer Membrane Vesicle Vaccine Reveals Broad and Balanced Humoral Immunogenicity
The current resurgence of whooping cough is alarming, and improved pertussis vaccines are thought to offer a solution. Outer membrane vesicle vaccines (omvPV) are potential vaccine candidates, but omvPV-induced humoral responses have not yet been characterized in detail.
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A proteomic portrait of Mycobacterium tuberculosis
PhD Defence
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Multi-omics studies of the control of growth and antibiotic production of Streptomyces
Actinobacteria are Gram-positive bacteria that have a complex multicellular life cycle and are well known for their ability to produce a wide range of bioactive natural products (NPs).
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Reading through proteins with graphene: NWO Vidi grant awarded to Dr. Grégory Schneider
While there are numerous and extremely advanced methods to sequence the genome, only a few methods exist to sequence the proteome. The Vidi project of Grégory Schneider promises to shed light on the most difficult paradigm of proteomics: achieving an error-free determination of the sequence of single…
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Environmental and metabolomic study of antibiotic production by actinomycetes
Promotor: Prof.dr. G.P. van Wezel
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Systems pharmacology of the endocannabinoid system
In this thesis, a system pharmacology approach, integrating metabolomics, pharmacology and chemical biology, was applied to understand and modulate the endocannabinoid system across different model systems (cells, zebrafish, mice and humans).
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The Palaeoproteomic Identification of Pleistocene Hominin Skeletal Remains:
Towards a Biological Understanding of the Middle to Upper Palaeolithic Transition
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Integrated omics approaches for systems understanding of adverse outcome pathways
Pathways of toxicity
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Statistical Methods for Mass Spectrometry-based Clinical Proteomics
PhD Defence
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Activity-based proteomics of the endocannabinoid system
PhD Defence
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Innovative strategies to clinically characterize the human tear proteome
PhD Defence
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Highly Selective, Reversible Inhibitor Identified by Comparative Chemoproteomics Modulates Diacylglycerol Lipase Activity in Neurons, J. Am.
Diacylglycerol lipase (DAGL)-α and -β are enzymes responsible for the biosynthesis of the endocannabinoid 2-arachidonoylglycerol (2-AG). Selective and reversible inhibitors are required to study the function of DAGLs in neuronal cells in an acute and temporal fashion, but they are currently lacking.…
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Relative quantification of proteasome activity by activity-based protein profiling and LC-MS/MS
Activity-based protein profiling (ABPP) is a functional proteomics technique for directly monitoring the expression of active enzymes in cell extracts and living cells. The technique relies on irreversible inhibitors equipped with reactive groups (warheads) that covalently attach to the active site…
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Activity-Based Proteasome Profiling
Promotor: H.S. Overkleeft, Co-promotor: B.I. Florea
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Activity-based protein profiling in drug discovery
In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.
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Sarantos Kostidis
Dr. in Biomedical NMR spectroscopy, LUMC, Leiden - The Netherlands
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Development of an Activity-Based Probe and In Silico Design Reveal Highly Selective Inhibitors for Diacylglycerol Lipase-α in Brain, Angew. Chem
A model method: A strategy that combines a knowledge-based in silico design approach and the development of novel activity-based probes (ABPs) for the detection of endogenous diacylglycerol lipase-α (DAGL-α) is presented. This approach resulted in the rapid identification of new DAGL-α inhibitors with…
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Expertise
The CMCB brings together a diverse range of unique expertise in microbial cell biology. Members of the CMCB investigate both model and non-model organisms, bacteria and archaea, pathogens and non-pathogens.
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Martin Giera
Assistant Professor at the Metabolomics group, LUMC, The Nederlands
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Expertise
The CIGR brings together a diverse range of unique expertise in genome research rooted in biology, chemistry and physics. Members of the CIGR investigate genome folding and genome transactions. An important aspect is direct as well as long term relevance for medicine. The available expertise extends…
- Research facilities
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Bioinformatic approaches to identify genomic, proteomic, and metabolomic biomarkers for the metabolic syndrome
PhD Defence
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Medical Biochemistry
Medical Biochemistry is a section of the Leiden Institute of Chemistry at Leiden University. The section is headed by Prof. Dr. Hans Aerts.
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Activity-based protein profiling reveals off-target proteins of the FAAH inhibitor BIA 10-2474, SCIENCE, 2017
The drug BIA 10-2474 inhibits fatty acid amide hydrolase (FAAH), a lipase that degrades a specific endocannabinoid. On the basis of this activity, BIA 10-2474 was being developed as a potential treatment for anxiety and pain. In a phase 1 trial of the drug, one subject died, and four others suffered…
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On Terminal Alkynes That Can React with Active-Site Cysteine Nucleophiles in Proteases
Active-site directed probes are powerful in studies of enzymatic function. We report an active-site directed probe based on a warhead so far considered unreactive.
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Vaccine delivery
Vaccination via the skin or the mucosae
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A community effort to assess and improve drug sensitivity prediction algorithms
Source: Nature Biotechnology, Volume 2014, Issue June (2014)
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Rapid and profound rewiring of brain lipid signaling networks by acute diacylglycerol lipase inhibition
Diacylglycerol lipases (DAGLα and DAGLβ) convert diacylglycerol to the endocannabinoid 2-arachidonoylglycerol. Our understanding of DAGL function has been hindered by a lack of chemical probes that can perturb these enzymes in vivo.
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Screening of animal venoms for antimicrobial compounds
Can animal venoms yield novel antimicrobial peptides?
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Rob van der Heijden
PhD at Leiden University and Bruker - the Netherlands
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Development of kinase inhibitors and activity-based probes
Promotor: H.S. Overkleeft, J. Neefjes, Co-promotor: M. van der Stelt
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Group benefits from genomic instability: a tale of antibiotic warriors in Streptomyces
Streptomyces are filamentous bacteria that produce more than two-thirds of known antibiotics.
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Bioactive Molecules in Animal Sciences
Animal Sciences’ contribution to the Bioactive Molecules research theme includes research on molecules from natural sources, such as plants, insects, and snake venom, with the aim to identify novel anti-cancer, anti-inflammatory, anti-microbial, and anti-diabetic agents.
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Chemical Biology
Chemical biology research at the Leiden Institute of Chemistry is aimed at understanding biological processes at the molecular level to strengthen the knowledge base of human health and disease. The approach to achieve this goal is a fundamental chemical one; with the aid of chemical probes biological…
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Molecular Physiology
Molecular Physiology is a research group at the Leiden Institute of Chemistry, headed by Prof. Mario van der Stelt. The research aim is to design, synthesize and apply new chemical tools to answer important biological and medical questions. We use activity-based protein profiling, assay development,…
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SDS-PAGE at the nanoscale: A nanorecorder for single molecule protein sequencing with graphene
Can we find new chemical and biological sensing routes on the edge and surface of graphene to improve the potential of graphene to act as a sensor?
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The Netherlands X-omics Initiative
The Netherlands X-omics Initiative is a new facility as part of the National Roadmap for Large-Scale Research Infrastructures. It is partly funded by NWO with a total budget of 40 million euro. The project started on September 2018 and will last for ten years.