The current hepatitis B vaccines need to be stored and transported under refrigerated conditions (2-8 degrees C). This dependence on a cold-chain is highly challenging in areas where hepatitis B virus infections are endemic.

The Biologics Formulation Group is led by Prof. Wim Jiskoot. A major challenge in the formulation of biopharmaceuticals is to overcome their inherent instability during production, storage, transportation, handling and administration. We are studying underlying mechanisms of degradation of biopharmaceuticals…

Formulation of biopharmaceuticals

Vaccination is the most effective method to prevent influenza infection. However, current influenza vaccines have several limitations. Relatively long production times, limited vaccine capacity, moderate efficacy in certain populations and lack of cross-reactivity are important issues that need to be…

Vaccines are an extraordinary category of biopharmaceuticals. They are usually prophylactic and come in many types, from whole bacteria to peptides and nucleic acids. They are very cost effective and almost every human on the planet is vaccinated. The latter mandates that vaccines have to be extremely…

Promotor: W. Jiskoot, Co-promotor: A. Hawe

Promotors: W. Jiskoot; G.J. Witkamp, Co-promotor: M.R. Nejadnik

Promotoren: G.F.A. Kersten, W. Jiskoot, Co-promotor: J-P. Amorij

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In this study, we evaluated the influence of supercritical carbon dioxide (scCO2) spray drying conditions, in the absence of organic solvent, on the ability to produce dry protein/trehalose formulations at 1:10 and 1:4 (w/w) ratios.

The potential of bioneedles to deliver influenza vaccines was investigated.

The stratum corneum is the outermost skin layer and consists of dead cells embedded in a lipid matrix.

Liposomes hold great promise for peptide-based personalized cancer vaccination, especially when administered intradermally. However, current liposome preparation methods are very time consuming (resulting in long development times per formulation), whereas personalized cancer vaccination requires very…

This thesis describes the development of improved formulations and alternative delivery strategies for polio vaccination.

Promotor: W. Jiskoot, Co-promotor: H.A. Every

PURPOSE: In the present study we investigated the root-cause of an interference signal (100-200 nm) of sugar-containing solutions in dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) and its consequences for the analysis of particles in biopharmaceutical drug products.

Atherosclerosis is a chronic inflammatory disease affecting millions of people world-wide.

The aim of current study was to develop a dried inactivated polio vaccine (IPV) formulation with minimal loss during the drying process and improved stability when compared with the conventional liquid IPV.

The skin is our natural barrier and lipids are a key part of this barrier. In the outer skin layer, the stratum corneum (SC), lipids form a densely organized structure dependent on the composition of these lipids.

The authors in this volume explore how external innovations (products, technologies, services, institutions and processes) have been appropriated in African societies in order to be acceptable and relevant to local conditions, expectations and demands.

PURPOSE: To determine the effect of elasticity on the skin permeation of ketoprofen from surfactant-based vesicular formulations and elastic liposomes.

Promotores: W. Jiskoot, F. Ossendorp

Key publications of the Biologics Formulation group

Mouse models are commonly used to assess the immunogenicity of therapeutic proteins and to investigate the immunological processes leading to antidrug antibodies. The aim of this work was to develop a transgenic (TG) Balb/c mouse model for evaluating the immunogenicity of recombinant human insulin (insulin)…

Een onderzoek naar de formulering, structuur, rechtshistorische aard en sociale en religieuze achtergronden van Laat-Egyptische (demotische) tempeleden, aangevuld met 70 ongepubliceerde teksten.

Promotores: Wim Jiskoot; Ferry Ossendorp

Developing robust and comprehensive analytical methods to size and quantify subvisible particles in biopharmaceutical products.

An overview of the research at the Biologics Formulation group

Cell-based medicinal products (CBMPs) show great promise when it comes to the treatment of genetic disorders. However, there is limited knowledge about CBMP stability during their production, storage, and administration to patients. The main focus of my research is the characterization of CBMPs using…

This dissertation contains the further development of a method for linguistic-stylistic research, which provides a systematic means to interpret global impressions of a text through choices in formulation at word- and sentence level.

Promotor: Prof.dr. W. Jiskoot, Co-promotor: A. Kros

PhD Defence

Thomas is currently a Bachelor’s student at Leiden University Law School.

In this study an attempt has been made to describe the degree in which the new objectives of the school subjects Greek and Latin, as formulated at the theoretical level by institutes as curriculum planning committees, are are incorporated by the teachers in their actual teaching practice.

The potential of liposomal cancer vaccines

Because of their large surface area and immunological competence, mucosal tissues are attractive administration and target sites for vaccination.

Focus in skin research

In this thesis we will explore the use of fuzzy systems theory for applications in bioinformatics.

Choong-Yeol Kim defended his thesis on 14 November 2019

On 29 November 2018, Roberto van den Heuvel zijn defended his thesis 'Duidelijkheid van fiscale wetgeving'. The doctoral research was supervised by prof. dr. A.O. Lubbers en prof. dr. H. Vording.

Didactics

For effective cancer immunotherapy by vaccination, co-delivery of tumour antigens and adjuvants to dendritic cells and subsequent activation of antigen-specific cytotoxic T cells (CTLs) is crucial. In this study, a synthetic long peptide (SLP) harbouring the model CTL epitope SIINFEKL was encapsulated…

This dissertation reports on the requirements for the design and development of teacher assessments, and examines the possibility of developing an assessment procedure that complies with the formulated requirements.

PhD Defence

PhD Defence

Development of GHG calculator for bio-electricity and heat.

Seasonal influenza vaccines provide protection against matching influenza A virus (IAV) strains mainly through the induction of neutralizing serum IgG antibodies. However, these antibodies fail to confer a protective effect against mismatched IAV. This lack of efficacy against heterologous influenza…

Can international organisations be held responsible for violations of international law by peacekeepers in a peacekeeping operation?

The loss of T-cell homeostasis and tolerance towards self-antigens is the underlying cause of autoimmune and inflammatory diseases like atherosclerosis, rheumatoid arthritis or type I diabetes. Traditionally, the treatment of these diseases consisted on systemic immune suppression, which can entail…