The current hepatitis B vaccines need to be stored and transported under refrigerated conditions (2-8 degrees C). This dependence on a cold-chain is highly challenging in areas where hepatitis B virus infections are endemic.

A major challenge in the formulation of biopharmaceuticals is to overcome their inherent instability during production, storage, transportation, handling and administration. We are studying underlying mechanisms of degradation of biopharmaceuticals (including therapeutic proteins, vaccines and advanced…

Formulation of biopharmaceuticals

Vaccination is the most effective method to prevent influenza infection. However, current influenza vaccines have several limitations. Relatively long production times, limited vaccine capacity, moderate efficacy in certain populations and lack of cross-reactivity are important issues that need to be…

Vaccines are an extraordinary category of biopharmaceuticals. They are usually prophylactic and come in many types, from whole bacteria to peptides and nucleic acids. They are very cost effective and almost every human on the planet is vaccinated. The latter mandates that vaccines have to be extremely…

Promotor: W. Jiskoot, Co-promotor: A. Hawe

Promotors: W. Jiskoot; G.J. Witkamp, Co-promotor: M.R. Nejadnik

Promotoren: G.F.A. Kersten, W. Jiskoot, Co-promotor: J-P. Amorij

description that google uses to depict when in presented in search results

In this study, we evaluated the influence of supercritical carbon dioxide (scCO2) spray drying conditions, in the absence of organic solvent, on the ability to produce dry protein/trehalose formulations at 1:10 and 1:4 (w/w) ratios.

The potential of bioneedles to deliver influenza vaccines was investigated.

The stratum corneum is the outermost skin layer and consists of dead cells embedded in a lipid matrix.

Liposomes hold great promise for peptide-based personalized cancer vaccination, especially when administered intradermally. However, current liposome preparation methods are very time consuming (resulting in long development times per formulation), whereas personalized cancer vaccination requires very…

Promotor: W. Jiskoot, Co-promotor: H.A. Every

This thesis describes the development of improved formulations and alternative delivery strategies for polio vaccination.

PURPOSE: In the present study we investigated the root-cause of an interference signal (100-200 nm) of sugar-containing solutions in dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) and its consequences for the analysis of particles in biopharmaceutical drug products.

Atherosclerosis is a chronic inflammatory disease affecting millions of people world-wide.

The aim of current study was to develop a dried inactivated polio vaccine (IPV) formulation with minimal loss during the drying process and improved stability when compared with the conventional liquid IPV.

Moving Week will take place between 20-21 August 2022. In order to prepare yourself for your Moving Day, we want to share with you some more info, and what to expect before this date. DUWO - our student housing partner that deals with LUC's housing will be in touch with you in the summer to start…

The skin is our natural barrier and lipids are a key part of this barrier. In the outer skin layer, the stratum corneum (SC), lipids form a densely organized structure dependent on the composition of these lipids.

The authors in this volume explore how external innovations (products, technologies, services, institutions and processes) have been appropriated in African societies in order to be acceptable and relevant to local conditions, expectations and demands.

Particles are omnipresent in biopharmaceutical products. In protein-based therapeutics such particles are generally associated with impurities, either derived from the drug product itself (e.g. protein aggregates), or from extrinsic contaminations (e.g. cellulose fibers).

PURPOSE: To determine the effect of elasticity on the skin permeation of ketoprofen from surfactant-based vesicular formulations and elastic liposomes.

Promotores: W. Jiskoot, F. Ossendorp

Key publications of the Biologics Formulation group

The walking club is a sub-group of ProParte.

Mouse models are commonly used to assess the immunogenicity of therapeutic proteins and to investigate the immunological processes leading to antidrug antibodies. The aim of this work was to develop a transgenic (TG) Balb/c mouse model for evaluating the immunogenicity of recombinant human insulin (insulin)…

Een onderzoek naar de formulering, structuur, rechtshistorische aard en sociale en religieuze achtergronden van Laat-Egyptische (demotische) tempeleden, aangevuld met 70 ongepubliceerde teksten.

Developing robust and comprehensive analytical methods to size and quantify subvisible particles in biopharmaceutical products.

Promotores: Wim Jiskoot; Ferry Ossendorp

In this book liposomes are explored as delivery system for allergen-specific immunotherapy. Both cationic and anionic formulations are prepared with Bet v 1, one of the allergens in birch pollen.

Cell-based medicinal products (CBMPs) show great promise when it comes to the treatment of genetic disorders. However, there is limited knowledge about CBMP stability during their production, storage, and administration to patients. The main focus of my research is the characterization of CBMPs using…

This dissertation contains the further development of a method for linguistic-stylistic research, which provides a systematic means to interpret global impressions of a text through choices in formulation at word- and sentence level.

Promotor: Prof.dr. W. Jiskoot, Co-promotor: A. Kros

PhD Defence

PhD Defence

In this study an attempt has been made to describe the degree in which the new objectives of the school subjects Greek and Latin, as formulated at the theoretical level by institutes as curriculum planning committees, are are incorporated by the teachers in their actual teaching practice.

Thomas is currently a Bachelor’s student at Leiden University Law School.

The potential of liposomal cancer vaccines

This paper, written by Silvia D'Amato & Andrea Terlizzi, investigates the extent to which the European Union is strategically engaging against terrorism.

On 29 November 2018, Roberto van den Heuvel zijn defended his thesis 'Duidelijkheid van fiscale wetgeving'. The doctoral research was supervised by prof. dr. A.O. Lubbers en prof. dr. H. Vording.

The research described in this thesis shows that hypercholesterolemia, a well-established risk factor for atherosclerosis, can impact skin lipid pool and barrier function already at young age. In the field of atherosclerosis, we showed that the small peptide Lyp-1 can be used as a targeting molecule…

Because of their large surface area and immunological competence, mucosal tissues are attractive administration and target sites for vaccination.

In this thesis we will explore the use of fuzzy systems theory for applications in bioinformatics.

Choong-Yeol Kim defended his thesis on 14 November 2019

Focus in skin research

For effective cancer immunotherapy by vaccination, co-delivery of tumour antigens and adjuvants to dendritic cells and subsequent activation of antigen-specific cytotoxic T cells (CTLs) is crucial. In this study, a synthetic long peptide (SLP) harbouring the model CTL epitope SIINFEKL was encapsulated…

This dissertation reports on the requirements for the design and development of teacher assessments, and examines the possibility of developing an assessment procedure that complies with the formulated requirements.

Development of GHG calculator for bio-electricity and heat.