Hass

The aim of the research presented in this thesis was to develop new methods forchallenging systems in liquid-state NMR using paramagnetic effects generated by thetwo-armed probe CLaNP-5.

Multicopper oxidase, laccase, can efficiently reduce oxygen to water and are mostly used in the enzymatic biofuel cells.

Promotor: Prof.dr. M. Ubbink

Promotor: Prof.dr. M. Ubbink

Promotor: Prof.dr. M. Ubbink

Nuclear Magnetic Resonance (NMR) Spectroscopy is a non-destructive analytical technique that is used to study the nature and characteristics of molecules with an atomic level precision. It has a wide range of applications especially in synthetic chemistry, biological and biochemical research groups…

A network combining 9 academic research groups and 4 collaborating industrial companies will train the next generation of PhD students and post-doctoral researchers, in developing and applying novel experimental and theoretical methods in the NMR spectroscopy of systems containing paramagnetic metals.…

The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy.

Promotor: M. Ubbink, Co-promotor: G. Siegal

Due to their central physiological roles in living organisms, retaining beta-glycosidases have been the subject of tremendous research efforts to examine their structure/function relation using numerous biophysical and biochemical approaches.

NMR theory can be tough. Luckily, most NMR theory can be visualized to aid you in understanding. The animations below show precession with a 180 degree pulse, J-coupling and T1/T2 relaxation.

Protein–protein complexes are formed via transient states called encounter complexes that greatly influence the formation of the stereospecific complex.

A full overview of MacBio PhD Theses.

The Paramagnetic NMR Facility Leiden has been established to provide support to researchers who want to apply paramagnetic NMR spectroscopy to biomolecules. Support is offered in the design and synthesis of paramagnetic probes, either for general application (such as lanthanide tags and spin labels)…

Promotor: M. Ubbink, Co-promotor: G. Siegal

Nature uses a special class of histone proteins, histone variants, to modulate the properties of chromatin at defined genomic locations.

Promotor: T.H. Oosterkamp

PhD Defence

PhD Defence

Parassign is a program to assign protein nuclei solely on the basis of pseudocontact shifts (PCS). The PCS from several paramagnetic tags are required, in order to produce several restraints per nucleus.

Macromolecular Biochemistry is a section of the Leiden Institute of Chemistry at Leiden University, comprising the PIs Marcellus Ubbink, Remus Dame, Aimee Boyle and Lars Jeuken.

Promotores: Prof.dr. H.J.M. de Groot, Prof.dr. J. Matysik (Universitaet Leipzig)

Huub de Groot is professor in Biophysical Organic Chemistry. With his team he works in the field of photosynthesis and artificial photosynthesis. The molecular basis for photosynthesis is formed by protein complexes and organelles that contain chlorophyll molecules. The antenna systems herein capture…

Paramagnetism. An obscure occult practice? No. It’s one of the newest scientific methods for mapping the behaviour of proteins with great precision. Marcellus Ubbink has been awarded 3 million euro by NWO and Leiden University to purchase a very powerful instrument. The goal: to acquire greater insight…

Climate change is a challenge for both current and future generations. New biological resources have to be developed in order to meet the demand for energy as well as the demand for food.

PNAS publication on protein encounter complexes

Science, Leiden Institute of Chemistry (LIC)

Light-Harvesting Complex II (LHCII) is responsible for light absorption and excitation energy transfer in plants and photosynthetic algae, while in high light it undergoes conformational changes by which it quenches excitations to prevent photodamage.

First author Rubin Dasgupta, PhD student at the Biophysical Organic Chemistry & Macromolecular Biochemistry groups at the Leiden Institute of Chemistry has made the cover of the June edition of Solid State Nuclear Magnetic Resonance.

The long term goal of the Biophysical Organic Chemistry/SSNMR group, headed by Prof. Huub de Groot, is to reach an understanding of structure, dynamics and functional mechanisms of membrane proteins and self-organized biological assemblies and to translate this knowledge into new concepts for nano-devices,…

Special amplifying agents can make MRI scanners and NMR techniques hundreds of times more sensitive. Leiden physicists have now found a method to test their efficiency. More sensitive MRI scans could for example improve our understanding of cystic fibrosis or Parkinson’s disease. Publication in PCCP…

Dynamic nuclear polarization with paramagnetic agents can enhance nuclear magnetic resonance signals by orders of magnitude. The mechanism of enhancement depends a.o. on the magnetic resonance properties of the agents used. Electron spin relaxation times at NMR relevant magnetic field/microwave frequencies,…

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Chemical biology research at the Leiden Institute of Chemistry is aimed at understanding biological processes at the molecular level to strengthen the knowledge base of human health and disease. The approach to achieve this goal is a fundamental chemical one; with the aid of chemical probes biological…

The thesis describes the use of electron paramagnetic resonance (EPR) spectroscopy, in continuous wave and pulse modes, to address the interaction of α-Synuclein (αS) with membranes and the aggregation of αS.

Researchers led by Professor of Chemistry Marcellus Ubbink have recently published a study in Proceedings of the National Academy of Sciences (PNAS) about the dynamics of an important redox enzyme. This work was accomplished thanks to an NWO VICI subsidy granted to Professor Ubbink.

The National Roadmap for Large-Scale Research Facilities programme of NWO enables the building or renovation of research facilities with international allure. Leiden NMR groups take part in the uNMR-NL consortium coordinated by Marc Baldus (Utrecht University) that has been awarded almost 18 million…

Promotor: Prof.dr. E.J.J. Groenen

Promotor: Prof.dr. R. Verpoorte, Co-promotores: Dr. Y.H.Choi, N.R. Mustafa

PURPOSE: To determine the effect of elasticity on the skin permeation of ketoprofen from surfactant-based vesicular formulations and elastic liposomes.

Factors affecting galanthamine production in Narcissus

Phospholipase A/acyltransferase 3 (PLAAT3) and PLAAT4 are enzymes involved in the synthesis of bioactive lipids. Despite sequential and structural similarities, the two enzymes differ in activity and specificity.

In this thesis, we show how MRFM can usefully contribute to the field of condensed-matter.

Promotors: Prof.dr. P.G.L. Klinkhamer, Prof.dr. P.M. Brakefield

Researchers from the Leiden Institute of Chemistry show proof of principle that live-cell structural changes and metabolic processes can be followed in real time with NMR spectroscopy. They performed their study on photosynthetic green algae that are metabolically flexible and can sustain energy generation…

Decentralized plug and play systems for energy production are the future picture of our society. Artificial photosynthetic systems are used for this purpose.

Promotor: G.P. van Wezel, Co-promotor: Y.H. Choi

Promotor: H.J.M. de Groot, Co-Promotor: A. Alia