177 search results for “paramagnetic nmr” in the Public website
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Protein motions revealed by paramagnetic NMR spectroscopy
Hass
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Applications of paramagnetic NMR spectroscopy for protein research
The aim of the research presented in this thesis was to develop new methods forchallenging systems in liquid-state NMR using paramagnetic effects generated by thetwo-armed probe CLaNP-5.
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Classical and paramagnetic NMR spectroscopy applied to different protein systems
Promotor: Prof.dr. M. Ubbink
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Making the invisible visible: paramagnetic NMR and the transient protein complex
Promotor: Prof.dr. M. Ubbink
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of the cytochrome P450cam-putidaredoxin complex determined by paramagnetic NMR spectroscopy and crystallography
Promotor: Prof.dr. M. Ubbink
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NMR Facility
Nuclear Magnetic Resonance (NMR) Spectroscopy is a non-destructive analytical technique that is used to study the nature and characteristics of molecules with an atomic level precision. It has a wide range of applications especially in synthetic chemistry, biological and biochemical research groups…
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Pushing the envelope of nuclear magnetic resonance spectroscopy for paramagnetic systems
A network combining 9 academic research groups and 4 collaborating industrial companies will train the next generation of PhD students and post-doctoral researchers, in developing and applying novel experimental and theoretical methods in the NMR spectroscopy of systems containing paramagnetic metals.…
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Design, synthesis and application of paramagnetic probes for protein structure studies
The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy.
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NMR studies of protein-small molecule and protein-peptide interactions
Promotor: M. Ubbink, Co-promotor: G. Siegal
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Understanding functional dynamics and conformational stability of beta-glycosidases
Due to their central physiological roles in living organisms, retaining beta-glycosidases have been the subject of tremendous research efforts to examine their structure/function relation using numerous biophysical and biochemical approaches.
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Marcellus Ubbink
Science
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Qing Miao
Science
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NMR Animations
NMR theory can be tough. Luckily, most NMR theory can be visualized to aid you in understanding. The animations below show precession with a 180 degree pulse, J-coupling and T1/T2 relaxation.
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PhD Theses
A full overview of MacBio PhD Theses.
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ParaNMR Facility
The Paramagnetic NMR Facility Leiden has been established to provide support to researchers who want to apply paramagnetic NMR spectroscopy to biomolecules. Support is offered in the design and synthesis of paramagnetic probes, either for general application (such as lanthanide tags and spin labels)…
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NMR structural studies of protein-small molecule interactions
Promotor: M. Ubbink, Co-promotor: G. Siegal
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Computational, biochemical, and NMR-driven structural studies on histone variant H2A.B
Nature uses a special class of histone proteins, histone variants, to modulate the properties of chromatin at defined genomic locations.
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Nuclear magnetic resonance force microscopy at millikelvin temperatures
Promotor: T.H. Oosterkamp
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applications of paramagnetic NMR spectroscopy for protein research
PhD Defence
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PARAssign
Parassign is a program to assign protein nuclei solely on the basis of pseudocontact shifts (PCS). The PCS from several paramagnetic tags are required, in order to produce several restraints per nucleus.
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Towards photo-CIDNP MAS NMR as a generally applicable enhancement method
Promotores: Prof.dr. H.J.M. de Groot, Prof.dr. J. Matysik (Universitaet Leipzig)
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Solid State NMR and modelling of photoinduced energy and electron transfer
Huub de Groot is professor in Biophysical Organic Chemistry. With his team he works in the field of photosynthesis and artificial photosynthesis. The molecular basis for photosynthesis is formed by protein complexes and organelles that contain chlorophyll molecules. The antenna systems herein capture…
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structure of whole-genome engineered Arabidopsis mutants : an HR-MAS NMR-based metabolomics approach
Climate change is a challenge for both current and future generations. New biological resources have to be developed in order to meet the demand for energy as well as the demand for food.
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Substantial investment in Leiden centre for NMR spectroscopy
Paramagnetism. An obscure occult practice? No. It’s one of the newest scientific methods for mapping the behaviour of proteins with great precision. Marcellus Ubbink has been awarded 3 million euro by NWO and Leiden University to purchase a very powerful instrument. The goal: to acquire greater insight…
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Identification of productive and futile encounters in an electron transfer protein complex
PNAS publication on protein encounter complexes
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structural study of light-harvesting complexes : an investigation with MAS-NMR
Light-Harvesting Complex II (LHCII) is responsible for light absorption and excitation energy transfer in plants and photosynthetic algae, while in high light it undergoes conformational changes by which it quenches excitations to prevent photodamage.
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Postdoctoral researcher
Science, Leiden Institute of Chemistry (LIC)
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Mechanism MRI amplifying agents explored
Special amplifying agents can make MRI scanners and NMR techniques hundreds of times more sensitive. Leiden physicists have now found a method to test their efficiency. More sensitive MRI scans could for example improve our understanding of cystic fibrosis or Parkinson’s disease. Publication in PCCP…
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Dynamic Nuclear Polarization agents
Dynamic nuclear polarization with paramagnetic agents can enhance nuclear magnetic resonance signals by orders of magnitude. The mechanism of enhancement depends a.o. on the magnetic resonance properties of the agents used. Electron spin relaxation times at NMR relevant magnetic field/microwave frequencies,…
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Parkinson’s protein α-synuclein: membrane interactions and fibril structure
The thesis describes the use of electron paramagnetic resonance (EPR) spectroscopy, in continuous wave and pulse modes, to address the interaction of α-Synuclein (αS) with membranes and the aggregation of αS.
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Macromolecular Biochemistry
Macromolecular Biochemistry is a section of the Leiden Institute of Chemistry at Leiden University, comprizing the PIs Marcellus Ubbink and Remus Dame.
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Research shows protein movement is important
Researchers led by Professor of Chemistry Marcellus Ubbink have recently published a study in Proceedings of the National Academy of Sciences (PNAS) about the dynamics of an important redox enzyme. This work was accomplished thanks to an NWO VICI subsidy granted to Professor Ubbink.
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Unveiling the invisible conformational landscape of cytochrome P450
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Spin-label EPR Approaches to Protein Interactions
Promotor: Prof.dr. E.J.J. Groenen
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17.9 million euro for national NMR Roadmap consortium
The National Roadmap for Large-Scale Research Facilities programme of NWO enables the building or renovation of research facilities with international allure. Leiden NMR groups take part in the uNMR-NL consortium coordinated by Marc Baldus (Utrecht University) that has been awarded almost 18 million…
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Metabolomic characteristics of Catharanthus roseus plants in time and space
Promotor: Prof.dr. R. Verpoorte, Co-promotores: Dr. Y.H.Choi, N.R. Mustafa
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Characterization and skin permeation of ketoprofen-loaded vesicular systems
PURPOSE: To determine the effect of elasticity on the skin permeation of ketoprofen from surfactant-based vesicular formulations and elastic liposomes.
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Factors affecting galanthamine production in Narcissus
Factors affecting galanthamine production in Narcissus
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Magnetic resonance force microscopy for condensed matter
In this thesis, we show how MRFM can usefully contribute to the field of condensed-matter.
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Non-target effects of GM potato: an eco-metabolomics approach
Promotors: Prof.dr. P.G.L. Klinkhamer, Prof.dr. P.M. Brakefield
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Connecting dots between natural and artificial Photosynthesis
Decentralized plug and play systems for energy production are the future picture of our society. Artificial photosynthetic systems are used for this purpose.
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Discovery of novel Antibiotics from Actinomycetes by Integrated Metabolomics & Genomics Approaches
Promotor: G.P. van Wezel, Co-promotor: Y.H. Choi
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The heart of oxygenic photosynthesis illuminated
Promotor: H.J.M. de Groot, Co-Promotor: A. Alia
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Wouter Elings
Science
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Huub de Groot
Science
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Anjali Pandit
Science
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Biological and Soft Matter Physics
Research groups in the Biological & Soft Matter Programme unravel mechanisms in biological processes and develop novel bio-inspired soft materials.
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Environmental and metabolomic study of antibiotic production by actinomycetes
Promotor: Prof.dr. G.P. van Wezel
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Dynamics of a β-lactamase
BlaC is the β-lactamase of Mycobacterium tuberculosis. We show that it can recover from inhibition by clavulanic acid and that phosphate helps it do so.
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Sarantos Kostidis
Dr. in Biomedical NMR spectroscopy, LUMC, Leiden - The Netherlands