The aim of the research presented in this thesis was to develop new methods forchallenging systems in liquid-state NMR using paramagnetic effects generated by thetwo-armed probe CLaNP-5.

Hass

Nuclear Magnetic Resonance (NMR) Spectroscopy is a non-destructive analytical technique that is used to study the nature and characteristics of molecules with an atomic level precision. It has a wide range of applications especially in synthetic chemistry, biological and biochemical research groups…

Promotor: Prof.dr. M. Ubbink

Promotor: Prof.dr. M. Ubbink

Due to their central physiological roles in living organisms, retaining beta-glycosidases have been the subject of tremendous research efforts to examine their structure/function relation using numerous biophysical and biochemical approaches.

Promotor: Prof.dr. M. Ubbink

The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy.

A network combining 9 academic research groups and 4 collaborating industrial companies will train the next generation of PhD students and post-doctoral researchers, in developing and applying novel experimental and theoretical methods in the NMR spectroscopy of systems containing paramagnetic metals.…

Promotor: M. Ubbink, Co-promotor: G. Siegal

Promotor: T.H. Oosterkamp

PhD Defence

Special amplifying agents can make MRI scanners and NMR techniques hundreds of times more sensitive. Leiden physicists have now found a method to test their efficiency. More sensitive MRI scans could for example improve our understanding of cystic fibrosis or Parkinson’s disease. Publication in PCCP…

A full overview of MacBio PhD Theses.

The Paramagnetic NMR Facility Leiden has been established to provide support to researchers who want to apply paramagnetic NMR spectroscopy to biomolecules. Support is offered in the design and synthesis of paramagnetic probes, either for general application (such as lanthanide tags and spin labels)…

Paramagnetism. An obscure occult practice? No. It’s one of the newest scientific methods for mapping the behaviour of proteins with great precision. Marcellus Ubbink has been awarded 3 million euro by NWO and Leiden University to purchase a very powerful instrument. The goal: to acquire greater insight…

PNAS publication on protein encounter complexes

Parassign is a program to assign protein nuclei solely on the basis of pseudocontact shifts (PCS). The PCS from several paramagnetic tags are required, in order to produce several restraints per nucleus.

Macromolecular Biochemistry is a section of the Leiden Institute of Chemistry at Leiden University, comprizing the PIs Marcellus Ubbink and Remus Dame.

Promotor: M. Ubbink, Co-promotor: G. Siegal

NMR theory can be tough. Luckily, most NMR theory can be visualized to aid you in understanding. The animations below show precession with a 180 degree pulse, J-coupling and T1/T2 relaxation.

Researchers led by Professor of Chemistry Marcellus Ubbink have recently published a study in Proceedings of the National Academy of Sciences (PNAS) about the dynamics of an important redox enzyme. This work was accomplished thanks to an NWO VICI subsidy granted to Professor Ubbink.

Climate change is a challenge for both current and future generations. New biological resources have to be developed in order to meet the demand for energy as well as the demand for food.

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Fundamental and clinical knowledge is needed to develop new, groundbreaking drugs. Physicians, pharmacists, biochemists, chemists and mathematicians from Leiden University and the Leiden University Medical Center (LUMC) work closely together in the hunt for the clues and building blocks that could lead…

Dynamic nuclear polarization with paramagnetic agents can enhance nuclear magnetic resonance signals by orders of magnitude. The mechanism of enhancement depends a.o. on the magnetic resonance properties of the agents used. Electron spin relaxation times at NMR relevant magnetic field/microwave frequencies,…

Huub de Groot is professor in Biophysical Organic Chemistry. With his team he works in the field of photosynthesis and artificial photosynthesis. The molecular basis for photosynthesis is formed by protein complexes and organelles that contain chlorophyll molecules. The antenna systems herein capture…

Promotores: Prof.dr. H.J.M. de Groot, Prof.dr. J. Matysik (Universitaet Leipzig)

Light-Harvesting Complex II (LHCII) is responsible for light absorption and excitation energy transfer in plants and photosynthetic algae, while in high light it undergoes conformational changes by which it quenches excitations to prevent photodamage.

In this thesis, we show how MRFM can usefully contribute to the field of condensed-matter.

Promotor: Prof.dr. E.J.J. Groenen

The thesis describes the use of electron paramagnetic resonance (EPR) spectroscopy, in continuous wave and pulse modes, to address the interaction of α-Synuclein (αS) with membranes and the aggregation of αS.

PURPOSE: To determine the effect of elasticity on the skin permeation of ketoprofen from surfactant-based vesicular formulations and elastic liposomes.

Promotor: Prof.dr. R. Verpoorte, Co-promotores: Dr. Y.H.Choi, N.R. Mustafa

Promotor: H.J.M. de Groot, Co-Promotor: A. Alia

Promotors: Prof.dr. P.G.L. Klinkhamer, Prof.dr. P.M. Brakefield

Promotor: G.P. van Wezel, Co-promotor: Y.H. Choi

Decentralized plug and play systems for energy production are the future picture of our society. Artificial photosynthetic systems are used for this purpose.

Dr Bela Bode receives a Feodor Lynen research fellowship for postdoctoral researchers by the Alexander von Humboldt-Foundation for development of new methods for signal enhancement in solid state NMR. He aims to create a specific analytical tool for surface studies and research on membranes and membrane…

Promotor: Prof.dr. G.P. van Wezel

The National Roadmap for Large-Scale Research Facilities programme of NWO enables the building or renovation of research facilities with international allure. Leiden NMR groups take part in the uNMR-NL consortium coordinated by Marc Baldus (Utrecht University) that has been awarded almost 18 million…

A new NMR microscope gives researchers an improved instrument to study fundamental physical processes. It also offers new possibilities for medical science, for example to better study proteins in Alzheimer patients’ brains. Publication in Physical Review Applied.

The studies presented in the work show the potential of the integrative use of biophysical data in defining the structural basis of protein interactions.

Anthe Janssen graduated ‘summa cum laude’ for the master’s programme Chemistry.He used NMR methods to gain insight in the syntheses and behavior of bio sugars. With this knowledge future therapeutics and research tools in hemical and medicinal biology can be developed. Anthe was awarded € 2.500.

Research groups in the Biological & Soft Matter Programme unravel mechanisms in biological processes and develop novel bio-inspired soft materials.