Hass

Multicopper oxidase, laccase, can efficiently reduce oxygen to water and are mostly used in the enzymatic biofuel cells.

The aim of the research presented in this thesis was to develop new methods forchallenging systems in liquid-state NMR using paramagnetic effects generated by thetwo-armed probe CLaNP-5.

Promotor: Prof.dr. M. Ubbink

A network combining 9 academic research groups and 4 collaborating industrial companies will train the next generation of PhD students and post-doctoral researchers, in developing and applying novel experimental and theoretical methods in the NMR spectroscopy of systems containing paramagnetic metals.…

The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy.

Promotor: Prof.dr. M. Ubbink

Promotor: Prof.dr. M. Ubbink

Nuclear Magnetic Resonance (NMR) Spectroscopy is a non-destructive analytical technique that is used to study the nature and characteristics of molecules with an atomic level precision. It has a wide range of applications especially in synthetic chemistry, biological and biochemical research groups…

Due to their central physiological roles in living organisms, retaining beta-glycosidases have been the subject of tremendous research efforts to examine their structure/function relation using numerous biophysical and biochemical approaches.

The thesis describes the use of electron paramagnetic resonance (EPR) spectroscopy, in continuous wave and pulse modes, to address the interaction of α-Synuclein (αS) with membranes and the aggregation of αS.

Promotor: Prof.dr. E.J.J. Groenen

Protein–protein complexes are formed via transient states called encounter complexes that greatly influence the formation of the stereospecific complex.

Promotor: T.H. Oosterkamp

Promotor: M. Ubbink, Co-promotor: G. Siegal

The Paramagnetic NMR Facility Leiden has been established to provide support to researchers who want to apply paramagnetic NMR spectroscopy to biomolecules. Support is offered in the design and synthesis of paramagnetic probes, either for general application (such as lanthanide tags and spin labels)…

A full overview of MacBio PhD Theses.

Research groups in the Biological & Soft Matter Programme unravel mechanisms in biological processes and develop novel bio-inspired soft materials.

A difficulty of studies on chemical kinetics are the reaction time scales and detection of their intermediates.

Parassign is a program to assign protein nuclei solely on the basis of pseudocontact shifts (PCS). The PCS from several paramagnetic tags are required, in order to produce several restraints per nucleus.

Dynamic nuclear polarization with paramagnetic agents can enhance nuclear magnetic resonance signals by orders of magnitude. The mechanism of enhancement depends a.o. on the magnetic resonance properties of the agents used. Electron spin relaxation times at NMR relevant magnetic field/microwave frequencies,…

Master projects are available in

PhD Defence

PhD Defence

This thesis focuses on amyloid proteins, a class of proteins that convert into amyloid fibrils.

Develop EPR instrumentation and methodology to investigate intrinsically disordered proteins (IDPs).

PNAS publication on protein encounter complexes

Science, Leiden Institute of Chemistry (LIC)

PhD Defence

Special amplifying agents can make MRI scanners and NMR techniques hundreds of times more sensitive. Leiden physicists have now found a method to test their efficiency. More sensitive MRI scans could for example improve our understanding of cystic fibrosis or Parkinson’s disease. Publication in PCCP…

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First author Rubin Dasgupta, PhD student at the Biophysical Organic Chemistry & Macromolecular Biochemistry groups at the Leiden Institute of Chemistry has made the cover of the June edition of Solid State Nuclear Magnetic Resonance.

The Biological- and Soft-Matter Physics Master specialisation is one of the two programmes Leiden offers in experimental Physics. The programme can to a high degree be tailored to individual needs and interests.

A full overview of MCBIM PhD theses.

Chemical biology research at the Leiden Institute of Chemistry is aimed at understanding biological processes at the molecular level to strengthen the knowledge base of human health and disease. The approach to achieve this goal is a fundamental chemical one; with the aid of chemical probes biological…

Chemical biology research at the Leiden Institute of Chemistry is aimed at understanding biological processes at the molecular level to strengthen the knowledge base of human health and disease. The approach to achieve this goal is a fundamental chemical one; with the aid of chemical probes biological…

The long term goal of the Biophysical Organic Chemistry/SSNMR group, headed by Prof. Huub de Groot, is to reach an understanding of structure, dynamics and functional mechanisms of membrane proteins and self-organized biological assemblies and to translate this knowledge into new concepts for nano-devices,…

Researchers led by Professor of Chemistry Marcellus Ubbink have recently published a study in Proceedings of the National Academy of Sciences (PNAS) about the dynamics of an important redox enzyme. This work was accomplished thanks to an NWO VICI subsidy granted to Professor Ubbink.

A new paramagnetic probe has been synthesized and applied to proteins.

Paramagnetism. An obscure occult practice? No. It’s one of the newest scientific methods for mapping the behaviour of proteins with great precision. Marcellus Ubbink has been awarded 3 million euro by NWO and Leiden University to purchase a very powerful instrument. The goal: to acquire greater insight…

The Leiden Institute of Chemistry offers a unique tailor-made programme in Life Science and Technology. You can adapt the programme to your own interests and ambitions with the help of your own personal mentor. Your study will focus on either a Biomedical or Molecular approach of Life Sciences. During…

The Chemistry research in Leiden is conducted in two major research areas: Chemical Biology and Energy & Sustainability. You will be part of one of our multidisciplinary research teams.

Research projects for students of the University of Leiden and other Dutch Universities are often available at the MacBio group. The research lines are also described in the Research section. For details of specific projects contact the supervisor (Ubbink, Dame, Boyle).

Aceruloplasminemia is a very rare, genetic disease accompagnied with iron accumulation that causes movement disorder and brain damage at early age.

For many years, scientists have observed a correlation between Alzheimer’s disease and a surplus of iron in the brain. However, a causal link between the two has not been proven yet. We lack knowledge concerning the specific form of iron that is involved in the development of neurodegenerative diseases.…

Dr Bela Bode receives a Feodor Lynen research fellowship for postdoctoral researchers by the Alexander von Humboldt-Foundation for development of new methods for signal enhancement in solid state NMR. He aims to create a specific analytical tool for surface studies and research on membranes and membrane…

There are many complex links in the chain that provides patients with new drugs: from fundamental science, to clinical tests, to production. The entire chain can be found in Leiden. Leiden University, the Leiden University Medical Center (LUMC) and the businesses at the Leiden Bio Science Park (LBSP)…

Physicists Lucia Bossoni and Martina Huber have been awarded an NWA Ideeëngenerator-grant for research into iron nanoparticles in the brain. These nanoparticles may be linked to air pollution and Alzheimer's disease.