Nuclear Magnetic Resonance (NMR) Spectroscopy is a non-destructive analytical technique that is used to study the nature and characteristics of molecules with an atomic level precision. It has a wide range of applications especially in synthetic chemistry, biological and biochemical research groups…

Hass

The aim of the research presented in this thesis was to develop new methods forchallenging systems in liquid-state NMR using paramagnetic effects generated by thetwo-armed probe CLaNP-5.

Promotor: M. Ubbink, Co-promotor: G. Siegal

Multicopper oxidase, laccase, can efficiently reduce oxygen to water and are mostly used in the enzymatic biofuel cells.

Promotor: Prof.dr. M. Ubbink

Nature uses a special class of histone proteins, histone variants, to modulate the properties of chromatin at defined genomic locations.

Promotor: Prof.dr. M. Ubbink

Promotor: Prof.dr. M. Ubbink

Promotor: M. Ubbink, Co-promotor: G. Siegal

Promotores: Prof.dr. H.J.M. de Groot, Prof.dr. J. Matysik (Universitaet Leipzig)

Huub de Groot is professor in Biophysical Organic Chemistry. With his team he works in the field of photosynthesis and artificial photosynthesis. The molecular basis for photosynthesis is formed by protein complexes and organelles that contain chlorophyll molecules. The antenna systems herein capture…

Light-Harvesting Complex II (LHCII) is responsible for light absorption and excitation energy transfer in plants and photosynthetic algae, while in high light it undergoes conformational changes by which it quenches excitations to prevent photodamage.

Climate change is a challenge for both current and future generations. New biological resources have to be developed in order to meet the demand for energy as well as the demand for food.

A network combining 9 academic research groups and 4 collaborating industrial companies will train the next generation of PhD students and post-doctoral researchers, in developing and applying novel experimental and theoretical methods in the NMR spectroscopy of systems containing paramagnetic metals.…

The National Roadmap for Large-Scale Research Facilities programme of NWO enables the building or renovation of research facilities with international allure. Leiden NMR groups take part in the uNMR-NL consortium coordinated by Marc Baldus (Utrecht University) that has been awarded almost 18 million…

Paramagnetism. An obscure occult practice? No. It’s one of the newest scientific methods for mapping the behaviour of proteins with great precision. Marcellus Ubbink has been awarded 3 million euro by NWO and Leiden University to purchase a very powerful instrument. The goal: to acquire greater insight…

Due to their central physiological roles in living organisms, retaining beta-glycosidases have been the subject of tremendous research efforts to examine their structure/function relation using numerous biophysical and biochemical approaches.

In this thesis, we show how MRFM can usefully contribute to the field of condensed-matter.

Researchers from the Leiden Institute of Chemistry show proof of principle that live-cell structural changes and metabolic processes can be followed in real time with NMR spectroscopy. They performed their study on photosynthetic green algae that are metabolically flexible and can sustain energy generation…

First author Rubin Dasgupta, PhD student at the Biophysical Organic Chemistry & Macromolecular Biochemistry groups at the Leiden Institute of Chemistry has made the cover of the June edition of Solid State Nuclear Magnetic Resonance.

Promotor: Prof.dr. R. Verpoorte, Co-promotores: Dr. Y.H.Choi, N.R. Mustafa

PURPOSE: To determine the effect of elasticity on the skin permeation of ketoprofen from surfactant-based vesicular formulations and elastic liposomes.

Factors affecting galanthamine production in Narcissus

Phospholipase A/acyltransferase 3 (PLAAT3) and PLAAT4 are enzymes involved in the synthesis of bioactive lipids. Despite sequential and structural similarities, the two enzymes differ in activity and specificity.

Promotors: Prof.dr. P.G.L. Klinkhamer, Prof.dr. P.M. Brakefield

The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy.

Decentralized plug and play systems for energy production are the future picture of our society. Artificial photosynthetic systems are used for this purpose.

Promotor: H.J.M. de Groot, Co-Promotor: A. Alia

Protein–protein complexes are formed via transient states called encounter complexes that greatly influence the formation of the stereospecific complex.

Dr. in Biomedical NMR spectroscopy, LUMC, Leiden - The Netherlands

Promotor: G.P. van Wezel, Co-promotor: Y.H. Choi

Promotor: Prof.dr. G.P. van Wezel

Dr. at Max Planck Institute for chemical Ecology, Jena - Germany

A full overview of MacBio PhD Theses.

Research at the BPOC/SSNMR group is comprised of the following research themes:

Plants, algae and cyanobacteria have the amazing capacity to perform delicate photophysical and photochemical processes of light capture, excitation transfer, charge separation and catalysis in fluctuating living environments. As a consequence, photosynthetic proteins and membranes form dynamic architectures.…

Contact Biophysical Organic Chemistry/Solid State NMR

Dr. Doris M. Jacobs, Lead Scientist at Unilever R&D, Wageningen, The Netherlands.

Promotor: T.H. Oosterkamp

Dynamic nuclear polarization with paramagnetic agents can enhance nuclear magnetic resonance signals by orders of magnitude. The mechanism of enhancement depends a.o. on the magnetic resonance properties of the agents used. Electron spin relaxation times at NMR relevant magnetic field/microwave frequencies,…

PhD at Université de Picardie Jules Verne, Amiens - France

How do you fit two meters of DNA in a tiny compartment and at the same time are able to access the right parts of it at the right times?

Parassign is a program to assign protein nuclei solely on the basis of pseudocontact shifts (PCS). The PCS from several paramagnetic tags are required, in order to produce several restraints per nucleus.

BlaC is the β-lactamase of Mycobacterium tuberculosis. We show that it can recover from inhibition by clavulanic acid and that phosphate helps it do so.

The Paramagnetic NMR Facility Leiden has been established to provide support to researchers who want to apply paramagnetic NMR spectroscopy to biomolecules. Support is offered in the design and synthesis of paramagnetic probes, either for general application (such as lanthanide tags and spin labels)…