Ramautar

Promotor: Prof.dr. T. Hankemeier, Co-promotor: T.H. Reijmers

The BioMedical Metabolomics Facility Leiden (BMFL) offers a highly structured environment for advanced metabolomics studies. BMFL builds on fully validated, state-of-the-art platforms that each cover a part of the human metabolism and together span the complete human metabolome.

Development of micro-scale analytical tools to be used to address relevant biomedical questions in the field of neuroscience, and other application areas intrinsically dealing with small sample amounts.

Promotor: Prof.dr. T. Hankemeier, Co-Promotor: R.J. Vreeken

Advanced mass spectrometry of glycosphingolipids takes the central stage in this thesis. Investigations focus on characterization of glycosphingolipid metabolism in health and disease with emphasis to the detection and accurate quantitation of known and so far unknown glycosphingolipids and closely

Promotor: Prof.dr. T. Hankemeier, Co-promotor: Dr. Theo Reijmers

Promotor: Prof.dr. T. Hankemeier, Co-Promotores: T. Reijmers, L. Coulier

Metabolomicshas emerged as an important discipline to study molecular and cellular processes in living cells and organisms with the ultimate aim to obtain an answer to a given biological/clinical question.

In the field of metabolomics, capillary electrophoresis-mass spectrometry (CE-MS) has become a very useful analytical technique for the profiling of highly polar and charged metabolites in biological samples. In order to highlight the unique features of CE-MS for metabolomics studies, Dr. Rawi Ramautar

Promotores: T. Hankemeier; R. Berger, Co-promotor: R.J. Vreeken

In this thesis, a system pharmacology approach, integrating metabolomics, pharmacology and chemical biology, was applied to understand and modulate the endocannabinoid system across different model systems (cells, zebrafish, mice and humans).

Live from the ASMS Conference 2015 in Saint Louis: Baljit Ubhi, our Metabolomics Specialist, has a coffee with Rawi Ramautar, Assistant Professor at the Leiden Academic Center for Drug Research.

Promotor: A.P. IJzerman

Promotor: Prof.dr. T. Hankemeier, Co-promotor: R.J. Vreeken

Promotor: A.P. IJzerman

PhD Defence

PhD Defence

By measuring proteins in tears, ophthalmologists can more easily diagnose dry eyes (Dry Eye Disease). Peter Raus, a Belgian ophthalmologist and PhD student at the Institute of Biology Leiden, developed a new method for protein determination in tear fluid. The technique is also promising for the early

PhD Defence

PhD Defence

PhD Defence

PhD Defence

PhD Defence

Key publications of the Biomedical Microscale Analytics group.

Our facilities cover:

Promotor: Prof.dr. G.P. van Wezel

We are developing biology-driven platforms to measure metabolites based on liquid chromatography-mass spectrometry (LC-MS), gas chromatography-MS (GC-MS), and capillary electrophoresis-MS (CE-MS).

What are the functions of the endocannabinoid system?

Netherton Syndrome (NTS) is a rare genetic skin disease caused by mutations in the serine protease inhibitor Kazal-type 5 gene, which encodes the lympho-epithelial Kazal-type-related inhibitor. NTS patients have a profound impaired skin barrier function. Because SC lipids play a crucial role in the

Can animal venoms yield novel antimicrobial peptides?

Towards a Biological Understanding of the Middle to Upper Palaeolithic Transition

Activity-based protein profiling (ABPP) is a functional proteomics technique for directly monitoring the expression of active enzymes in cell extracts and living cells. The technique relies on irreversible inhibitors equipped with reactive groups (warheads) that covalently attach to the active site

A major goal of ABS research is to overcome one of the major bottle-necks in metabolomic research: the lack of a high-throughput infrastructure in which thousands of samples can be processed in a standardized and cost-effective way (max. a few tens of Euro’s per sample for a full metabolome profile).

The Biomedical Microscale Analytics group is led by Dr. Rawi Ramautar. In present-day metabolomics, and bioanalysis in general, the analytical toolbox used encounters difficulties for the analysis of limited amounts of biological samples. As a result, a significant number of crucial biomedical/clinical

Actinomycetes produce a plethora of bioactive natural products, including two thirds of all antibiotics used in the clinic, and many other medically important molecules such as those with anticancer, antifungal or immunosuppressant activity. We seek to unravel the regulatory networks that control the

The Leiden Institute of Chemistry actively participates in several national research schools for MSc and PhD students. These schools are consortia that stimulate collaboration between research groups of the different participating Dutch universities. The research schools also offer advanced training

A research goal for ABS is to develop miniaturized platforms for research in (stem)cell, in-vitro systems and application in clinical and preventive research. Combined with the high throughput, this development will make it possible to study the dynamics of pathogenesis in human and cellular models

Which metabolites of sponge are influenced by individual environmental or biological factors?

Can we develop a generic method for Quan/Qual analysis of small molecule drugs, their metabolites and endogenous metabolites in (pre-)clinical samples?

The current resurgence of whooping cough is alarming, and improved pertussis vaccines are thought to offer a solution. Outer membrane vesicle vaccines (omvPV) are potential vaccine candidates, but omvPV-induced humoral responses have not yet been characterized in detail.

Promotor: Prof.dr. K.H. Kuijken, Co-Promotor: Dr. H.Hoekstra

Papers written on the occasion of the 30th anniversary of the institute of Prehistory, Volume II.

Can a new proteomics workflow be developed to link genes and gene clusters to bioactive molecules, identify novel compounds and enhance the production in the Streptomyces lividans enzyme?

The System Biomedicine and Pharmacology specialisation consists of compulsory and optional components.

The current projects of the Molecular Physiology group focus on kinases and proteins of the endocannabinoid system.