Promotor: H.S. Overkleeft, Co-promotor: B.I. Florea

What are the values that Leiden University cherishes? What does the University stand for and what does it consider important?

Less than 1 in 10 drug candidates that enter phase 1 clinical trials actually gets approved for human use.

Leiden University has been an international university from its founding in 1575.

Students are educated to become academic professionals and responsible and engaged citizens who are internationally and interculturally competent

Physics and Engineering Approaches to Biomedicine and Pharmacology

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

To this day, all cyclophellitol-based inhibitors and ABPs have been close analogues of their natural substrate counterparts. As a result, these probes showed high selectivity towards their target glycosidases.

In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.

The Low Frequency Array enables studies of low-frequency carbon radio recombination lines with unprecedented sensitivity, spectral and spatial resolution.

The reintegration of high profile ex-offenders frequently receives great political and public attention. While this attention, more often than not in the form of negative sentiments, may be understandable from a moral perspective, it raises several important questions: how do such offenders reintegrate…

Current research at the Skin Barrier and Vaccination group.

Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.

Promotor: Prof.dr. E.F. van Dishoeck, Prof.dr. F.F.S. van der Tak (RUG)

Promotor: Prof.dr. F.S. de Boer, Co-promotor: D. Clarke

Supervisor: Anna Vlachodimou

Due to the fact that the Frisian lands lacked major political centers in the Middle Ages and also because most of its monasteries suffered complete destruction in the second half of the 16th century, relatively few written documents concerning medieval Frisian history have survived. To analyze them…

Politically speaking, the Frisian coastal area constitutes a special case in late medieval Europe since it was not subject to an overlord as it withstood feudalization in the 13th century. Its many sub regions, which were dominated by elites of small noblemen and freeholders, long time succeeded in…

Promotor: H.S. Overkleeft, Co-Promotor: B.I. Florea

Promotor: Prof.dr. T. Hankemeier, Co-Promotor: R.J. Vreeken

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-promotor: B.I. Florea

Supervisor: Maarten Doornbos

The key question addressed in this family study is whether the psychophysiological and neurocognitive abnormalities often reported in SAD patients are heritable and can thus be found in family members of SAD patients as well. Determination of heritability of these deficiencies is essential for endophenotyping.…

Science, Leiden Institute of Advanced Computer Science (LIACS)

Activity-based protein profiling (ABPP, also termed chemical proteomics), is one of the pillars of chemical biology, and at LED3 we have taken it to the next level. ABPP allows the assessment of protein function in live cells and tissues, which means that the activity of a complete protein family can…

The close interaction between organic chemi stry and biology goes back to the late 18th century, when the modern natural sciences began to take shape. After synthetic organic chemistry arose as a discipline, organic chemists almost immediately began to pursue the synthesis of naturally occurring compounds,…

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

Since my master's thesis on the landed property of the Frisian monasteries in the Middle Ages I am highly interested in the do ut des-aspects of the relation between religious houses and the lay world. Key words here are: property, power, patronage and the role of religious institutions in the 'salvation…

Abdessamad El Amraoui defended his thesis on 6 Mai 2015.

How are digital risk-profiles developed and used in practice?

Activity-based protein profiling (ABPP) is a functional proteomics technique for directly monitoring the expression of active enzymes in cell extracts and living cells. The technique relies on irreversible inhibitors equipped with reactive groups (warheads) that covalently attach to the active site…

Supervisor: Andrea Martella

The current resurgence of whooping cough is alarming, and improved pertussis vaccines are thought to offer a solution. Outer membrane vesicle vaccines (omvPV) are potential vaccine candidates, but omvPV-induced humoral responses have not yet been characterized in detail.

Source: Biosens Bioelectron, Volume 74, pp. 233-42 (2015)

Description of Triple bottom line approach for risk-benefit analysis of genetically modified crops.

Ceramides (CERs), cholesterol, and free fatty acids (FFAs) are the main lipid classes in human stratum corneum (SC, outermost skin layer), but no studies report on the detailed analysis of these classes in a single platform.

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The narrow but long stretched stript of Frisian land along the North Sea was already occupied before Roman times. However, man repeatedly suffered setbacks when he tried to extend his living space, both in the tidal marshes and the peat area south of them.

The cannabinoid CB2 receptor (CB2R) represents a promising therapeutic target for various forms of tissue injury and inflammatory diseases. Although numerous compounds have been developed and widely used to target CB2R, their selectivity, molecular mode of action and pharmacokinetic properties have…

Historical geo data are gaining in importance. Provided that they are exactly geo referenced, they can be stored into a GIS and thus be combined with all kinds of maps (topographical, pedological, etc.) and datasets. This makes it possible to analyze historical developments in space and time on a detailed…

Fighting for the faith, caring for the sick, and praying for the soul of their benefactors were the main tasks of the military orders, who since the time of the crusades were well represented in the Netherlands in the Middle Ages, including the Frisian lands. Especially the Hospitallers and the Teutonic…

The Dutch atlas with pesticides concentrations in surface waters have been overhauled. This new, free accessible, website is in line with the European Framework Directive on Water (In Dutch: KRW).

Schools of secondary education are assigned to (further) develop a curriculum vision regarding three functions of education (Sectorakkoord VO, 2014; 2018).: qualification, socialization, and subjectification (Biesta, 2015). This research project is aimed at developing instruments that help schools identify,…

Vast, often populated, areas in dryland ecosystems face the dangers of desertification.

A high-end label: Cyclophellitol aziridine-type activity-based probes allow for ultra-sensitive visualization of mammalian β-glucosidases (GBA1, GBA2, GBA3, and LPH) as well as several non-mammalian β-glucosidases (see picture). These probes offer new ways to study β-exoglucosidases, and configurational…

Vitamin A or retinol is essential in embryonic development, the visual cycle and the immune system.

This article on private active cyber defense by Dennis Broeders is part of a special issue of the Journal of Cybersecurity, based on a selection of contributions taken from the 2019 Conference on Cyber Norms organized by The Hague Program for Cyber Norms.

Chemical tools to monitor drug-target engagement of endogenously expressed protein kinases are highly desirable for preclinical target validation in drug discovery. Here, we describe a chemical genetics strategy to selectively study target engagement of endogenous kinases.

The drug BIA 10-2474 inhibits fatty acid amide hydrolase (FAAH), a lipase that degrades a specific endocannabinoid. On the basis of this activity, BIA 10-2474 was being developed as a potential treatment for anxiety and pain. In a phase 1 trial of the drug, one subject died, and four others suffered…