94 search results for “homologous recombination” in the Public website
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Role of non-homologous end-joining in T-DNA integration in Arabidopsis thaliana
Promotor: P. J. J. Hooykaas Co-promotor: B. S de Pater
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Efficient gene-targeting during meiosis
Efficient gene-targeting during meiosis
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Strategies for the improvement of genome editing in Arabidopsis thaliana
Increasing the efficiency of gene targeting (GT) as a genome editing tool in plants has been an important goal in plant biotechnology.
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T-DNA integration and DNA repair of DSBs in plants
Identification and characterization of components of DNA repair pathways and their role in Agrobacterium T-DNA integration and repair of CRISPR/Cas induced DSBs.
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Resistance to PARP inhibition by DNA damage response alterations in BRCA1/2-deficient tumors
Inactivating mutations in BRCA1 or BRCA2 genes predispose to several types of cancer.
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Cancer chess: molecular insights into PARP inhibitor resistance
The clinical potential of applying synthetic lethality to cancer treatment is famously demonstrated by the BRCA1/PARP1 paradigm: a tumor specific defect in BRCA1 – a component of the DNA double-strand break (DSB) repair pathway homologous recombination (HR) – results in a remarkable sensitivity to PARP1…
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How Bio-questionable are the Different Recombinant Human Erythropoietin Copy Products in Thailand?
The high prevalence of pure red cell aplasia in Thailand has been associated with the sharp increase in number of recombinant human erythropoietin (rhEPO) copy products, based on a classical generic regulatory pathway, which have entered the market.
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A fresh view on carbon radio recombination lines powered by LOFAR
The Low Frequency Array enables studies of low-frequency carbon radio recombination lines with unprecedented sensitivity, spectral and spatial resolution.
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From Star-formation to Recombination: Expanding our View of the Radio-Recombination-Line Universe
The origin and evolution of galaxies are closely tied to the cyclic feedback processes between stars and the interstellar medium (ISM).
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relation between dynamics and activity of phospholipase A/acyltransferase homologs
Phospholipase A/acyltransferase 3 (PLAAT3) and PLAAT4 are enzymes involved in the synthesis of bioactive lipids. Despite sequential and structural similarities, the two enzymes differ in activity and specificity.
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Development of a Transgenic Mouse Model to Study the Immunogenicity of Recombinant Human Insulin
Mouse models are commonly used to assess the immunogenicity of therapeutic proteins and to investigate the immunological processes leading to antidrug antibodies. The aim of this work was to develop a transgenic (TG) Balb/c mouse model for evaluating the immunogenicity of recombinant human insulin (insulin)…
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Exploring the Role of Homologous Recombination Deficiency and BRCA1/2 Mutations in Endometrial Cancer
PhD Defence
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Phyto-pharmaceuticals and gene targeting
We exploit secondary metabolites of plant to develop pharmaceutical compounds and we genetically modify plant genomes to improve crops.
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Software developments in automated structure solution and crystallographic studies of the Sso10a2 and human C1 inhibitor protein
Promotor: J.P. Abrahams, Co-Promotor: N.S. Pannu
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Macromolecular Biochemistry
Macromolecular Biochemistry is a section of the Leiden Institute of Chemistry at Leiden University, comprising the PIs Marcellus Ubbink, Remus Dame, Aimee Boyle and Lars Jeuken.
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Advances in GPCR Modeling Evaluated by the GPCR Dock 2013 Assessment: Meeting New Challenges
Source: Structure , Volume 22, Issue 8, pp. 1120-1139 (2014)
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Studies on the Pathogenesis of Chronic Kidney Disease
In this thesis, two potential therapeutic targets for diabetic nephropathy were dentified and investigated. First, we show that glomerular clusterin is upregulated in diabetic nephropathy and demonstrated that recombinant clusterin protein can protect the podocytes against oxidative stress in vitro.…
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Summer school
In the week of July 1st - 5th there will be a summer school on "Data science for dynamical systems" at the Lorentz Center in Leiden.
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John van Noort Lab (Chromatin Dynamics)
Chromatin is an ubiquitous protein-DNA complex that forms the structural basis of DNA condensation in all eukaryotic organisms.
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Helmut Schiessel Group (Theoretical Physics of Life Processes)
The group Theoretical Physics of Life Processes, led by Helmut Schiessel, focuses on the physics of chromatin, the DNA-protein complex that fills the nuclei of eukaryotic cells.
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Exploring structure dependencies of gas-surface interactions with curved single crystals
Curved single crystals provide variable, but well-defined surface structures.
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Role of non-homologous end-joining in T-DNA integration in Arabidopsis thaliana
PhD Defence
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relation between dynamics and activity of phospholipase A/acyltransferase homologs
PhD Defence
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Functions of P38 and ERK kinases in zebrafish early development
Promotor: Prof.dr. H.P. Spaink Co-promotor: Dr. B.E. Snaar
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Chemical genetic approaches for target validation
Drug development is a time- and resource-consuming process that starts with the discovery and validation of a (protein) target that contributes to pathogenesis or disease progression.
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Structure-kinetics relationships of Capadenoson derivatives as adenosine A1 receptor agonists
Source: Eur J Med Chem, Volume 101, pp. 681-91 (2015)
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Quantitative live cell imaging of glucocorticoid receptor dynamics in the nucleus
In this thesis, the focus lies on studying glucocorticoid receptor dynamics in living cells with the aim of understanding how this transcription factor finds its DNA target sites to regulate transcription.
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Neurogenomics of vocal learning
How does FoxP1 affect auditory perception on a behavioural and genomic level?
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Dynamic organization of bacterial chromatin by DNA bridging proteins
Bacteria often experience external challenges, such as changes in environmental conditions or attacks by bacteriophages.
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Convergent molecular evolution of toxins in the venom of advanced snakes (Colubroidea)
The explosive radiation and diversification of the advanced snakes (superfamily Colubroidea) was associated with changes in all aspects of the shared venom system. Morphological changes included the partitioning of the mixed ancestral glands into two discrete glands devoted for production of venom ormucous…
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From Star-formation to Recombination: Expanding our View of the Radio-Recombination-Line Universe
PhD Defence
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A fresh view on carbon radio recombination lines powered by LOFAR
PhD Defence
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Chromatin organization & dynamics (Dr. Remus Dame)
Throughout all domains of life, from bacteria and archaea to eukaryotes, genomes adopt well-organized three-dimensional structures that can change in space and time to accommodate preferred transcriptional programs for environmental adaptation, the maintenance of cellular identity and differentiation…
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The evolution of shell form in tropical terrestrial microsnails
Promotor: Prof.dr. M. Schilthuizen
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Agrobacterium-mediated protein therapy for genome editing
Is translocation of nucleases possible via Agrobacterium T4SS and is it efficient enough for mutagenesis?
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A study on PsbS and its role as a pH sensor
Solar energy harnessed by plants and algae has great potential to be converted into biofuels for future generations.
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Histone-DNA assemblies in archaea. Shaping the genome on the edge of life
All life on earth contains DNA, which is used to store biological information. Organisms compact their DNA in order for it to fit inside their cell(s).
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Interaction of oxygen and carbon monoxide with Pt(111) at intermediate pressure and temperature: revisiting the fruit fly of surface science
Promotor: M.T.M. Koper, Co-promotors: A.I. Yanson, L.B.F. Juurlink
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Projects
Research projects
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Bacterial Chromatin
The relative simplicity of the bacterial cell, short generation times and well defined and inexpensive culturing conditions have significantly contributed to our understanding of many complex biological systems. Yet the workings of the bacterial genome, seemingly impossibly compressed within a tiny…
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Studies of dust and gas in the interstellar medium of the Milky Way
Promotor: Prof.dr. A.G.G.M. Tielens, Co-Promotor: J.B.R. Oonk
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Steps in gas-surface reactions
Heterogeneous catalysis is essential to many industrial applications. These catalysts are often comprised of supported nanoparticles, which contain various different surface sites.
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Supramolecular peptide amphiphile nanoparticles as a novel allergy vaccine platform
Bet v1, the major allergen in birch pollen, is one of the main causes of allergies such as asthma and rhino-conjunctivitis. Additionally it is the main cause of birch pollen allergy in humans.
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Ultrasensitive in situ visualization of active glucocerebrosidase molecules
Deficiency of glucocerebrosidase (GBA) underlies Gaucher disease, a common lysosomal storage disorder. Carriership for Gaucher disease has recently been identified as major risk for parkinsonism. Presently, no method exists to visualize active GBA molecules in situ. We here report the design, synthesis…
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Top Lectures: Holger Puchta
Lecture
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Bernd Schneider
Dr. at Max Planck Institute for chemical Ecology, Jena - Germany
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Astrophysical plasma modeling of the hot Universe: Advances and challenges in high-resolution X-ray spectroscopy
Hot astrophysical plasma is ubiquitous in the Universe, from comets in our Solar system to the largest scale structures -- the cosmic web filaments. These hot plasmas, with the temperature of a few millions of degrees, are often observed in the X-ray wavelength range.
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Chemical biology of sphingolipids: fundamental studies and clinical applications
Overkleeft
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Chemokine signaling in Tuberculosis and Salmonella infection
Who benefits from CXCR/CXCL chemokine signaling during infection: host or pathogen?
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Functions of autophagy in the filamentous fungus Aspergillus niger
What are the functions of autophagy in the filamentous fungus Aspergillus niger?