High throughput microscopy

Promotor: B. van de Water

There is an urgent need for more physiologically relevant cell culture methods to guide compound selection in pre-clinical stages of the drug development pipeline.

Can chemical safety assessment be improved with zebrafish embryo-based reporter assays?

The Microscopy Unit houses, maintains and coordinates most of the microscopy equipment of the IBL. The available equipment ranges from conventional light and fluorescence microscopes, to confocal laser scanning and electron microscopes. In addition, infrastructure is available for histology, including…

Promotor: B. van de Water, Co-Promotores: J.H.N. Meerman, F.J. Verbeek

The challenge of achieving fast quantification in metabolomics is the presence of severe matrix effects during the MS analysis of complex samples.

Promotor: Prof.dr. T. Hankemeier, Co-Promotor: R.J. Vreeken

How can zebrafish research help to understand and fight human cancer?

A major goal of ABS research is to overcome one of the major bottle-necks in metabolomic research: the lack of a high-throughput infrastructure in which thousands of samples can be processed in a standardized and cost-effective way (max. a few tens of Euro’s per sample for a full metabolome profile).…

Promotor: Prof.dr. H.P. Spaink, Co-promotor: Prof. dr. A.H. Meijer

We have studied shape with a particular focus on the zebrafish model system. The shape is an essential appearance of the phenotype of a biological specimen and it can be used to read out a current state or response or to study gene expression.

Promotor: T. Schmidt

Liposomes hold great promise for peptide-based personalized cancer vaccination, especially when administered intradermally. However, current liposome preparation methods are very time consuming (resulting in long development times per formulation), whereas personalized cancer vaccination requires very…

Our research in the area of safety sciences aims to increase the mechanistic understanding of cellular toxicity of drugs and, in a broader sense, chemicals.

Electron microscopy has become an extremely important techniquein a wide variety of elds.

The aim of this facility is to accelerate biomedical research by providing access to advanced light and electron microscopy and by sharing expertise about sample preparation, imaging modalities, data storage and image analysis.

Promotor: B. van de Water

Science

Science, Institute of Biology Leiden (IBL)

This thesis described the development of novel scanning tunneling microscopy techniques to investigate strongly correlated electronic states in quantum matter.

Intrigued by the way cells autonomously regulate their fate, we strive to understand and visualize cellular processes as the basis of Cell Signaling.

The High Troughput Microscopy Dutch Flagship Node that includes the Leiden Cell Observatory Screening platform is now officially ratified as a Euro-BioImaging (EuBI) Node Candidate

Our Division has created and maintains the Visualisation Core Facilities of the LACDR. This is a multi-user facility offering basic and advanced microscopic techniques to scientists of the LACDR.

Discovery of metastasis promoting candidate drug targets

Promotor: Prof.dr. J.M. van Ruitenbeek, Prof.dr. R.M. Tromp

In this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms.

In this thesis, we describe the latest advances in SQUID-detected Magnetic Resonance Force Microscopy (MRFM).

Promotor: Prof.dr. R.M.H. Merks

PhD Defence

In this thesis, we show how MRFM can usefully contribute to the field of condensed-matter.

The dissertation describes TEM experiments on heterogeneous catalysts.

Promotores: Prof.dr. H.P. Spaink & Prof.dr. P.C.W. Hogendoorn, Co-promotor: Dr. M.J.M. Schaaf

Promotores: Prof.dr. C.U. Keller, Prof.dr. H.C. Gerritsen

Promotor: T.H. Oosterkamp

We explore the possibilities to combine magnetic resonance techniques with atomic force microscopy together in a single microscope: the MRI-AFM, also called Magnetic Resonance Force Microscopy (MRFM).

In this research group, headed by Bob Van de Water, cell signaling programs that underlie adverse drug reactions as well as cancer development and progression are unraveled. Adverse drug reactions involve cell injury in critical target organ cells which leads to the activation of cellular stress response…

Promotor: Prof.dr. J.N. Kok, Co-promotor: F.J. Verbeek

Establishing human induced pluripotent stem cell (iPSC) CRISPR-Cas9-based fluorescent reporter cell lines for NF-kB signalling. We will use this to enable studying the dynamic activation and downstream signalling of Toll-like receptor and cytokine signalling in iPSC-derived differentiated cells in a…

The purpose of this study was to investigate the formation and growth kinetics of complexes between proteins and oppositely charged polyelectrolytes.

This thesis investigates the relations between metabolism and anatomy through the use of Magnetic Resonance Imaging (MRI).

We focus on a better understanding of the mechanisms of cancer drug resistance and metastasis.

The Netherlands Organisation for Scientific Research (NWO) has made a subsidy of 17 million euros available to further develop a Netherlands network for electron microscopy (NEMI). The network comprises five UMCs and eight universities, with Utrecht in the coordinating role. From Leiden, the Institute…

To study how planetary systems come into existence we study much younger systems still in formation.

Our facilities cover:

Can drug screening for tuberculosis treatment be made more efficient?

Which immune mechanisms are involved in host defense against Staphylococcal infection?

High Performance Computing previously the domain of theoretical scientists and computer and software developers is becoming ever more important as a research tool in many research areas. A University facility, providing serious computational capabilities, combined with easy and flexible local access,…