946 search results for “heterologous protein” in the Public website
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PhD Theses
A full overview of MacBio PhD Theses.
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Chi-Lin Kuo
Science
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Research
Research at the Macromolecular Biochemistry group is comprised of the following research themes:
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Macromolecular Biochemistry
Macromolecular Biochemistry is a section of the Leiden Institute of Chemistry at Leiden University, comprising the PIs Marcellus Ubbink, Remus Dame, Aimee Boyle and Lars Jeuken.
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Master Projects
Master projects are available in
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Membrane Interactions
Membrane proteins and membrane interactions of proteins are studied by spin label EPR. Intact, native membranes, vesicles of all sizes and nano-disks can be investigated, because the size of the object does not matter in EPR. Whereas solution NMR is limited to micelles (a few nm in diameter), by EPR…
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Louise Jawerth Lab
Research in the Jawerth lab focuses on using principles from soft condensed matter physics to understand important biological materials.
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Putting a spin on it: amyloid aggregation from oligomers to fibrils
This thesis focuses on amyloid proteins, a class of proteins that convert into amyloid fibrils.
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Photosynthetic light reactions at the gold interface
Promotor: Prof.dr. T.J. Aartsma, Co-promotor: R.N. Frese
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Biological and Soft Matter Physics
Research groups in the Biological & Soft Matter Programme unravel mechanisms in biological processes and develop novel bio-inspired soft materials.
- Martina Huber Lab (EPR Group)
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Regulation of the arabidopsis AGC kinase PINOID by PDK1 and the microtubule cytoskeleton
Plants, are sessile organisms, have developed strategies to adapt to changes in their environment, in part by altering their growth and development.
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In vitro investigation of the photoprotection mechanism of Light Harvesting Complex II
Solar energy is used by photosynthetic organisms to drive energy required cellular processes. Is absorbed by two groups of pigments, located in the LHCs.
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Bachelor Projects
Bachelor projects are available in
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MediSURF: Bioactive surfaces for precision medicines
Is it possible to successfully design self-assembling nano-scale protein skeletons for use as a vaccine platform?
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Sticky & Dirty Proteins
PhD Defence
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NWO ECHO grant for research on fickle RNA production
Genes are active sometimes, and other times they remain dormant for a while. Leiden physicist John van Noort receives an NWO ECHO grant to find out how this happens.
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Chromatin organisation & dynamics
The genomic DNA of every organism is organized and compacted in order to fit inside the cell. This is achieved by the joint action of numerous architectural proteins that aid in folding the genome. Genome folding is tightly interconnected with transcription, with genes in certain regions being silenced,…
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Influenza T-cell epitope-loaded virosomes adjuvanted with CpG as a potential influenza vaccine
Influenza CD8(+) T-cell epitopes are conserved amongst influenza strains and can be recognized by influenza-specific cytotoxic T-cells (CTLs), which can rapidly clear infected cells. An influenza peptide vaccine that elicits these CTLs would therefore be an alternative to current influenza vaccines,…
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How Electrostatic Interactions Drive Nucleosome Binding of RNF168 & PSIP1
The studies presented in the work show the potential of the integrative use of biophysical data in defining the structural basis of protein interactions.
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Structure and regulation in photosynthesis
Plants, algae and cyanobacteria have the amazing capacity to perform delicate photophysical and photochemical processes of light capture, excitation transfer, charge separation and catalysis in fluctuating living environments. As a consequence, photosynthetic proteins and membranes form dynamic architectures.…
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Teaching
Research projects for students of the University of Leiden and other Dutch Universities are often available at the MacBio group. The research lines are also described in the Research section. For details of specific projects contact the supervisor (Ubbink, Dame, Boyle).
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Microbial Chemotaxis
In order to gain insight into the structure and function of the molecular complexes involved in chemotaxis, we use electron cryotomography (ECT). This technique allows us to directly study microbes in their native state at resolutions capable of visualizing individual proteins.
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Single gold nanorods in live cells
Visualization of biomolecules in living cells by tagging with gold nanoparticles.
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Proteochemometrics
Research question
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In shape for photoregulation
How does the photoregulation mechanism work in detail?
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Chemical Biology Lecture: Chemical Protein Synthesis - Providing Tailor-Made Proteins for Biology and Medicine
Lecture
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Unraveling cross-presentation pathways using a chemical biology approach
Is it possible for sugar patterns on cells to activate the immune system to combat cancer?
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Membrane Fusion Mediated Intracellular Delivery of Lipid Bilayer Coated Mesoporous Silica Nanoparticles
Alexander Kros and co-workers describe how mesoporous silica nanoparticles (MSNs) are delivered into cells via membrane fusion thereby omitting endocytosis pathways. Fusion is induced by a pair of complementary coiled-coil lipopeptides inserted into the membrane of cells and in the bilayer of lipid-coated…
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Chromatin organization & dynamics (Dr. Remus Dame)
Throughout all domains of life, from bacteria and archaea to eukaryotes, genomes adopt well-organized three-dimensional structures that can change in space and time to accommodate preferred transcriptional programs for environmental adaptation, the maintenance of cellular identity and differentiation…
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Cellular Forces: Adhering, Shaping, Sensing and Dividing
Promotor: Prof.dr. T. Schmidt
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Noonan and LEOPARD syndrome in zebrafish: molecular mechanisms and cardiac development
Promotor: Prof.dr. J. den Hertog
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Mechanical and genetics basis of cellularization and serosal window closure in Tribolium castaneum
The applications of studying the early development of insects range from agriculture to material science.
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In silico characterization of a GPCR focused library
Supervisor: Gerard van Westen
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Novel receptor concepts
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Our approach
Our group operates at the cross-roads of different disciplines: molecular and cellular microbiology, biochemistry, structural biology and biophysics. The group harbors expertise in a multitude of molecular and cellular approaches including:
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Targeting Human Proteasomes: Substrates, Inhibitors and Prodrugs
Large parts of the research described in this Thesis aims at the development of oligopeptide-masked toxins and their in situ immunoproteasome-mediated activation.
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PhD position to study regulation and transport of a novel anti-diabetic plant derived metabolite
Science, Institute of Biology Leiden (IBL)
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Protein Surface Recognition Using Designed Molecules
Lecture
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Mass spectrometry based protein profiling
PhD Defence
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Mass spectrometry based protein profiling
PhD Defence
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Yao Xiao
Plant researchers have been wrong for years about how plant growth and development are regulated, as Yao Xiao pointed out during his PhD project. They overlooked the role of a protein that turns out to be essential, just as it is in the development of animals and yeasts. In plants, it regulates the…
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Interview - Running around with Erlenmeyer flasks filled with yeast
In order to achieve a more sustainable food system, the food industry has to come up with new, environmentally friendly products. One aspect is producing proteins for meat substitutes. Bachelor’s student Rose Vossen modified, grew and studied different yeast strains to see whether they can be used to…
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Cryo Electron Tomography Studies On Bacterial Chemosensory Arrays
Bacterial chemosensory arrays are protein assemblies that are the key structural and functional component for motile bacteria to sense their internal or environmental chemical signals.
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Unveiling the invisible conformational landscape of cytochrome P450
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Cheminformatics: Analyzing small-molecule activity data
While bioinformatics methods deal with the analysis of sequence information (be it proteins or DNA), the field of cheminformatics is concerned with the analysis of small-molecule datasets.
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Bioorthogonal Antigens as Tool for Investigation of Antigen Processing and Presentation
In order to be able to develop effective medicine and treatments to prevent or cure autoimmune diseases or cancer we need to understand the mechanisms how they arise and what drives their course.Unravelling the fundamental molecular mechanisms influencing the onset and course of diseases such as allergies,…
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Determining the structure of a chemotaxis kinase complex with receptor mimetics and cryo-EM
Can we determine the 3D structure of a chemotaxis core complex using single-particle reconstructions via cryo-EM?
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Innovative strategies to clinically characterize the human tear proteome
Transplantation of labial salivary glands to the eyelids for patients with dry eye appears to give excellent results clinically.
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Chemical genetic approaches for target validation
Drug development is a time- and resource-consuming process that starts with the discovery and validation of a (protein) target that contributes to pathogenesis or disease progression.