The focus of the described research in this thesis is on the oxidative stress response (Nrf2 pathway). The aim of the research presented in this thesis is to obtain more information concerning microRNAs which are involved in the Nrf2 pathway, to determine and evaluate the application of microRNAs for…

The major and ultimate aim of metabolomics is to obtain an answer to a specific biological or clinical question.

In previous studies at our laboratory it was demonstrated that drug exposure of HepG2 cells can lead to an altered TNFα-induced NF-κB oscillatory phenotype, concurrent with a synergistically increased sensitivity for TNFα-induced apoptosis.

Promotor: B. van de Water

The aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds.

The way organisms develop from the initial single-cellular state to a complex final assembly like the human body, and how the final body is maintained throughout life, is one of the greatest mysteries and it’s understanding one of the biggest scientific challenges.

Synthetic hydrogels that mimic the natural extracellular matrix in the biophysical and biochemical cues it provides to cells are in high demand, however the cell phenotypes as they are observed in vivo in numerous cases have yet to be attained.

Despite being the object of intense study, embryonic development has been difficult to model due to a number of reasons. First, complex tissues can be comprised of many cell types, of which we probably only know a subset.

Decision support tool for cell therapy commercialization and business development

Leiden has a long history in the treatment of blood cell cancer. Research to find better therapies never stands still. One of the potential treatments currently being worked on is a ‘living medicine’.

Living cells on topographically-controlled substrates: Understanding Cell Motility in a Quasi-3D Environment

Mechanical interactions between cells and their environment play an important role in many biological processes.

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Science, Leiden Academic Centre for Drug Research (LACDR)

Promotor: Prof.dr. J. Kuiper, Co-Promotor: S.C.A. de Jager

A thorough understanding of the workings of the cell underly the understanding of disease at the molecular level. Revealing the principles of biochemical processes helps to identify the targets for the development of new drugs. At the Leiden Institute of Chemistry (LIC), we believe that the application…

BioSolar Cells is a five-year research project in which ten knowledge institutions and 45 private industries work together.

In this thesis an activity-based probe was discovered that could visualize the activity of PLAATs. With an optimized gel-based ABPP assay in hand, screening of a compound library led to the discovery of alpha-ketoamides as a hit for PLAAT3.

What distinguishes a tumour cell from a healthy cell? Researchers are trying to answer this question as precisely as possible. Certain differences could eventually lead to new therapies.

In this project innovative technology for photovoltaics cells is developed and analysis of ecological and health related risks are explicitly accounted for.

Cardiovascular disease is a major global burden and atherosclerosis is the main underlying pathological process.

The CMCB brings together microbial cell biology expertise in Leiden. It aims at optimal resonance between the groups and maximal exploitation of the available infrastructure and to develop innovative cross-boundary technology platforms.

Promotor: R.C.M. Pelger Co-Promotor: G. van der Pluijm

Almost all bacteria are enveloped by a cell wall that provides cellular protection.

Which brain cell does what, when Parkinson's disease arises? It won't be long before this jigsaw is solved piece by piece. Ahmed Mahfouz, computational biologist, combines bio-knowledge from Leiden with algorithms from Delft and is getting closer to finding the key.

The loss of T-cell homeostasis and tolerance towards self-antigens is the underlying cause of autoimmune and inflammatory diseases like atherosclerosis, rheumatoid arthritis or type I diabetes. Traditionally, the treatment of these diseases consisted on systemic immune suppression, which can entail…

Atherosclerosis, in particular rupture of atherosclerotic plaques, is the underlying cause of most heart attacks or strokes and is the leading cause of death worldwide.

Cardiovascular syndromes are the major cause of death in Western societies.

Cell adhesion signaling

Atherosclerosis is the most important underlying process that drives cardiovascular disease, and is characterized by an accumulation of cholesterol which triggers an inflammatory response in the vessel wall.

The word ¨pyroptosis¨ can be understood as ¨fiery falling¨, which describes the bursting of pro-inflammatory signals from the dying cell. Our observations indicate that pyroptosis also ¨spreads like wildfire¨ and once a cell dies via pyroptotic cell death, neighbouring cells are more prone to die as…

Atherosclerosis is the main underlying pathology of cardiovascular disease. Atherosclerosis is caused by an immune response which is directed against (modified) lipoproteins which accumulate in the vessel wall.

From molecule, to cell, to organism. The high-level drug research at the Cell Observatory aimes at reducing the spread of cancer and developing new medicines for tuberculosis.

Mechanobiology is a major area in biology, however, mechanics is currently a missing element in drug development pipelines. This has led to a high cost for the society. Mechanotoxicity of drugs often appears as a long-term side effect of drugs, and has led to removal of drugs from the market. Furthermore,…

We aim to engineer streptomycetes to fully exploit their potential for natural product productions, by a rational design and evolution approach.

Treatment with special stem cells seems to be a good option for suppressing the immune system in organ transplants. Researchers are trying to learn from processes that take place in the human body during pregnancy.

Source: Biosens Bioelectron, Volume 74, pp. 233-42 (2015)

Epithelial-mesenchymal plasticity (EMP) and tumor cell migration play an important role in cancer progression, and an improved understanding of the mechanisms underlying these concepts is essential for developing new targeted approaches.

A new therapy for the serious Graft-versus-Host disease

Visualization of biomolecules in living cells by tagging with gold nanoparticles.

Promotores: Prof.dr. M.K. Richardson, Prof.dr. H.P. Spaink

In this research project, we aimed to obtain more insight in the role of mast cells in the immune driven disorders rheumatoid arthritis and atherosclerosis.

What is the role of cell wall-deficiency in the persistence of tuberculosis?

A new type of tripodal squaramide-based supramolecular hydrogels is developed and studied.

During embryonic growth, cells proliferate, differentiate, and collectively migrate to form different tissues at the right position and time in the body.

Bacteria have the ability to alter their morphology in order to adapt to changing environments.

This functional genomics project aims at understanding the biology of the underlying mycelium differentiation and autolysis processes in much more detail.

Promotor: Huub J.M. de Groot, Co-promotor: Francesco Buda

Aspergillus niger is an important industrial producer of organic acids and enzymes producing large amounts of spent fungal biomass.