This thesis focuses on two processes involved in fighting infections: metabolism and immune cell motility and navigation.

Parkinson's disease is the second most common neurodegenerative disease in the world. One of its symptoms is the loss of dopaminergic neurons in the substantia nigra pars compacta.

Filamentous Actinobacteria, such as Streptomyces, produce a plethora of chemically diverse bioactive metabolites that have found applications across medicine, agriculture and biotechnology.

The aim of this thesis is to expedite and ensure the systematic accuracy of clearance scaling from adults to paediatric patients, with a special focus on drugs undergoing hepatic metabolism.

What are the principles of genome folding and dynamics in bacteria?

This research group, headed by Miranda van Eck, focusses on understanding the molecular mechanisms underlying cardiovascular and metabolic diseases. Cardiovascular disease constitutes a major health issue worldwide. Although significant knowledge has been obtained on the development of atherosclerotic…

The research described in this thesis has, using the zebrafish as a model system, shed new light on the intricate relationship between TB and DM2, in particular on the role of leptin, SHP-1 and glucocorticoids.

The facility develops and applies advanced, high-throughput methods for genomic research.

The four possible segments A, T, C and G that link together to form DNA molecules, and with their ordering encode genetic information, are not only different in name, but also in their physical and chemical properties.

In this thesis we present several methods to automate tomographic reconstruction algorithms and several novel tomographic reconstruction algorithms with the focus on being easily applicable and efficient to use.

How did toxin genes evolve from harmless physiological genes?

The CIGR brings together a diverse range of unique expertise in genome research rooted in biology, chemistry and physics. Members of the CIGR investigate genome folding and genome transactions. An important aspect is direct as well as long term relevance for medicine.

Vitamin A or retinol is essential in embryonic development, the visual cycle and the immune system.

Promotor: G.P. van Wezel, Co-promotor: Y.H. Choi

Which metabolites of sponge are influenced by individual environmental or biological factors?

Metabolic diseases are a burden on the European population and health care system. It is increasingly recognised that individual differences with respect to history, lifestyle, and genetic make-up affect disease progression and treatment response. A Systems Medicine approach, based on computational…

Is translocation of nucleases possible via Agrobacterium T4SS and is it efficient enough for mutagenesis?

Cholesterol influences many pathways, including serving as precursor for adrenal steroidogenesis. Imbalance of cholesterol levels has been implicated in several diseases including cardiovascular diseases and its underlying pathology, atherosclerosis.

Cardiovascular disease is the leading cause of death worldwide.

Promotor: K.J. Batenburg

Streptomyces are filamentous bacteria that produce more than two-thirds of known antibiotics.

Increasing the efficiency of gene targeting (GT) as a genome editing tool in plants has been an important goal in plant biotechnology.

An experimental approach to organic palaeolithic technology

Promotores: T. Hankemeier; R. Berger, Co-promotor: R.J. Vreeken

With tomography it is possible to reconstruct the interior of an object without destroying.

The research in this thesis is focused on tomographic reconstruction based on two imaging modalities in electron microscopy.

Sphingolipids are important membrane compounds with a variety of functions. In mammalian cells, different enzymes are involved in the metabolism of sphingolipids, but interruption of this metabolism process leads to different diseases.

We live in a world of things and people in the past must have been as closely entangled with their material surroundings as we are now. In the Laboratory for Artefact Studies Van Gijn takes a close look at the biographies of objects: what kind of raw material an object is made off and what is its provenience,…

Cardiovascular disease is the number one cause of death worldwide. The most important risk factor for developing this disease is high cholesterol levels in the blood.

This dissertation presents a comprehensive description of Xinka, an indigenous language from southeastern Guatemala. The description is based on a missionary grammar that is titled Arte de la lengua szinca and was written by the priest Manuel Maldonado de Matos around 1773.

Dorothea Schulz & Martin Hense

Acute cardiovascular clinical events such as myocardial infarction and cerebral stroke represent the major cause of death in Western societies. These pathologies are primarily resulting from atherosclerosis, a progressive condition characterized by the accumulation of lipids, immune cells, and fibrous…

Atherosclerosis is the main underlying pathology of cardiovascular disease. Atherosclerosis is caused by an immune response which is directed against (modified) lipoproteins which accumulate in the vessel wall.

The research is aimed at development of an efficient gene targeting method that allows controlled integration of DNA at a preselected site in the Trichoderma genome.

Growth and development affect the metabolism of drugs administered to neonates, infants, and children.

Promotor: Prof.dr. K.J. Batenburg

The chaotic years after the Civil War are often seen as a time of uniquely American idealism—a revolutionary attempt to rebuild the nation that paved the way for the civil rights movement of the twentieth century. But Adam Fairclough rejects this prevailing view, challenging prominent historians such…

Promotor: J.N. Kok, Co-promotor: M.S. Lew

Promotor: H.J.A. Röttgering, Co-promotor: M. Haverkorn

An assessment of Libyan legislation

Leiden University archaeologists are reconstructing houses from the Stone Age, using only resources available during that period. This method offers surprising insights into the ingenuity of our distant ancestors, and challenges existing archaeological views.

Some examples of research questions include: What framework of circular strategies should local practitioners use? How should practitioners organize their monitoring of the circular economy activities in the region of interest? What are the main products that have the most circular potential in the…

Can the venom of snakes, scorpions and other animals be sources of new antibiotics?

This project intends to provide an integrated linguistic assessment of the hypothesised migration route of the Tocharians.

From descriptive to physiological models that can predict oral absorption and elimination of CYP3A substrates across the pediatric age range.

The power of personalized nutrition lies in being able to conduct clinical research on healthy people while capturing metabolic markers sensitive to the impact of environmental and metabolic stressors (e.g. diet, changing sex hormones and the menstrual cycle).

This thesis investigates the relations between metabolism and anatomy through the use of Magnetic Resonance Imaging (MRI).

How to strike a balance between the sometimes conflicting stakes of individual, public and private data-producers and data-processors?

Leiden evolutionary biologist Maurijn van der Zee has co-operated on an enormous international project to describe the genetic material of the pea aphid Acyrthosiphon pisum. The more than 100 authors involved in the project have published their findings in the online magazine PLoS Biology.

Galaxy intrinsic alignments induce a major astrophysical contamination to weak gravitational lensing measurements and need to be modelled and mitigated when extracting cosmological information from such measurements.