1800 search results for “gene targeting” in the Public website
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Efficient gene-targeting during meiosis
Efficient gene-targeting during meiosis
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Exploring Grainyhead-like 2 target genes in breast cancer
The objective of this study was to investigate the expression and function of GRHL2 in different breast cancer subtypes.
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Phyto-pharmaceuticals and gene targeting
We exploit secondary metabolites of plant to develop pharmaceutical compounds and we genetically modify plant genomes to improve crops.
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Gene Shev
Faculteit Archeologie
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Efficient targeting of the Trichoderma genome for industrial protein engineering
The research is aimed at development of an efficient gene targeting method that allows controlled integration of DNA at a preselected site in the Trichoderma genome.
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Cancer Drug Target Discovery
We focus on a better understanding of the mechanisms of cancer drug resistance and metastasis.
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Chemical genetic approaches for target validation
Drug development is a time- and resource-consuming process that starts with the discovery and validation of a (protein) target that contributes to pathogenesis or disease progression.
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Chromatin dynamics in single genes
John van Noort
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Discovery of metastasis promoting candidate drug targets
Discovery of metastasis promoting candidate drug targets
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Horizontal gene transfer and spreading of biosynthetic gene clusters and antimicrobial resistance
Biosynthetic gene clusters (BGCs) for natural products are widespread in microbial genomes, and they are rapidly exchanged. This research assesses the factors that control the spread of BGCs and resistance genes in nature. This includes risk assessment for the spread of engineered DNA in nature.
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mutagenesis to identify breast cancer drivers and therapy resistance genes in mice
In this thesis, we used genetically engineered mouse models to identify genes and pathways that are involved in ILC formation and in the development of resistance to FGFR-targeted therapy.
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Strategies for the improvement of genome editing in Arabidopsis thaliana
Increasing the efficiency of gene targeting (GT) as a genome editing tool in plants has been an important goal in plant biotechnology.
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Paul Hooykaas
Science
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Germline variants in the mismatch repair genes
PhD Defence
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Targeted Therapy for Triple-Negative Breast Cancer
The research described in this thesis focused on identifying novel drug targets and synergistic combinations for triple-negative breast cancer (TNBC), a virulent subtype of breast cancer with a dismal prognosis and limited therapeutic options.
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Image analysis for gene expression based phenotype characterization in yeast cells
Promotores: T.H.W. Bäck, A. Plaat, Co-promotor: F.J. Verbeek
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TARGETBIO: Transmission of Antimicrobial Resistance Genes and Engineered DNA from Transgenic Biosystems in Nature
This project aims to assess the risk of spread of antimicrobial resistance genes in the environment derived from currently used synthetic biology approaches in the field of drug discovery.
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Targeting the adenosinergic system
Adenosine is an endogenous ligand which exerts its action by activating adenosine receptors (ARs), while its circulating levels are controlled via a variety of mechanism and proteins, amongst others the equilibrative nucleoside transporters (ENTs).
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Identification and characterization of developmental genes in streptomyces
Promotor: Prof.dr. G.P. van Wezel
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Cancer Drug Target Discovery
In this research group, headed by Prof. Erik Danen, the aim is to unravel cellular signaling mechanisms in normal and diseased cells, with a long-standing interest in cell adhesion signaling. In complex multicellular organisms such as ourselves, a division of labor emerges where different tissues and…
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Intervention targets in cognitive development
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Proteochemometric modelling coupled to in silico target prediction
An integrated approach for the simultaneous prediction of polypharmacology and binding affinity/potency of small molecules.
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The role of the Arabidopsis AHL15/REJUVENATOR gene in developmental phase transitions
This thesis describes the functional analysis of the Arabidopsis AHL15 gene.
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Liver X receptor: therapeutic targeting of common pathways in Psoriasis and Cardiovascular co-morbidities
Both psoriasis and atheroslcerosis are diseases characterized by a chronic and systemic inflammatory profile with local lipid disturbances that alter the barrier function of the skin (psoriasis) and cause the development of atherosclerotic plaques (atherosclerosis).
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Tom van der Wel
Science
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analysis of Dof transcription factors controlling heading date and PPDK gene expression in rice
Promotor: P.J.J. Hooykaas, Co-Promotores: P.B.F. Ouwerkerk, M. Wang
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Unravelling the genes responsible for life history traits in the giant woody cabbage (Brassica oleracea)
Which genes are involved in woodiness and associated traits such as drought tolerance, flowering time, stem elongation, life span, and plant herbivory, and how do these gene regulatory pathways overlap?
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Targeted biomolecule production for therapeutic use
We aim to develop a drug-delivery method based on the production of biomolecules directly at the target site.
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Drug-target binding kinetics in vivo
A next, general pharmacological problem to be tackled is how drug-target binding kinetics in vivo, affects target occupancy as an important indicator of the time-course of drug effects.
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ZF-CANCER - Developing high-throughput bioassays for human cancers in zebrafish
How can zebrafish research help to understand and fight human cancer?
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Functional characterization and unraveling the Gene Regulatory Networks (GRNs) of HD-Zip transcription factor HB40 (and HB22) in Arabidopsis
In this Ph.D. thesis, we characterized two novel upstream regulators of JUB1, HB40, and HB22, in Arabidopsis thaliana. In Chapter 2, we described functions of HB40 in modulating growth and development via the regulation of JUB1 and GA inactivation genes GA2OXs.
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Targeting Human Proteasomes: Substrates, Inhibitors and Prodrugs
Large parts of the research described in this Thesis aims at the development of oligopeptide-masked toxins and their in situ immunoproteasome-mediated activation.
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T-DNA integration and DNA repair of DSBs in plants
Identification and characterization of components of DNA repair pathways and their role in Agrobacterium T-DNA integration and repair of CRISPR/Cas induced DSBs.
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Using zebrafish to target the Achilles’ heel of cancer
Exploiting metabolic vulnerabilities to identify anticancer compounds in zebrafish synthetic lethality screens.
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CRISPR Cas-induced targeted mutagenesis with Agrobacterium mediated protein delivery
The RNA guided endonuclease based on the CRISPR/Cas system of Streptococcus pyogenus is a potent new tool for genome engineering in plants.
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A time-space translation mechanism for patterning the vertebrate anteroposterior axis
My PhD project studies how the temporally sequential Hox gene expression is regulated during head-tail patterning of the frog embryo.
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Epilepsy and anxiety: targeting a vicious cycle
How effective is eye movement desensitization and reprocessing therapy in targeting epilepsy-related anxiety?
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Preclinical validation of putative targets in cardiovascular and metabolic disease
Cardiovascular disease is the number one cause of death worldwide. The most important risk factor for developing this disease is high cholesterol levels in the blood.
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Image-based phenotypic screening for breast cancer metastasis drug target discovery
The main aim of this thesis was to unravel the signaling and regulatory networks that drive tumor cell migration during breast cancer metastasis.
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Novel role of the AT-HOOK MOTIF NUCLEAR LOCALIZED 15 gene in Arabidopsis meristem activity and longevity
Plant architecture has distinct forms in different plant species, but also within a species the finalarchitecture of a plant is determined by its gradual development and changes therein induced by environmental conditions during the plant’s life cycle.
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Identification of novel targets in prostate cancer progression
Promotor: Prof.dr. B. van de Water, Co-promotor: E.H.J. Danen
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Leiden researchers discover genes that affect spread of breast cancer
Researchers at Leiden University have mapped genes that cause breast cancer cells to migrate. The Leiden toxicologists Professor Bob van de Water, Dr Wies van Roosmalen, Dr Sylvia E. Le Dévédec and colleagues studied 1500 individual genes. They found eight, including the SRPK1 gene, that regulate the…
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Novel chemical tools for target validation in neuroinflammation
In what way can new tools and techniques be used to develop drug candidates for the treatment of neurological disorders?
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Novel target engagement biomarkers for better drug candidates
Is is possible to better predict the validity of drug candidates?
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Targeting of antigen-presenting cells with mannosylated conjugates
This thesis describes the development of a variety of mannosylated conjugates. Antigen presenting cells bear mannoside recognizing receptors that actively transport antigen into the cell. This thesis exploits this feature for the development of improved vaccines.
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Project TARGET: gun violence and firearms trafficking in Europe”
Project TARGET aims to improve our understanding of gun violence in Europe and the relationship between gun violence and firearms trafficking.
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The Role of Target Binding Kinetics in Drug Discovery
Source: ChemMedChem (2015)
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CD40-targeted dendritic cell delivery of PLGA-nanoparticle vaccines induce potent anti-tumor responses
Dendritic cells (DC) play a prominent role in the priming of CD8(+) T cells. Vaccination is a promising treatment to boost tumor-specific CD8(+) T cells which is crucially dependent on adequate delivery of the vaccine to DC.
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Regulation of nanoparticle biodistribution by scavenger endothelial cells (SECs)
A very large part of intravenously administered nanoparticles are cleared through the liver. Within the liver, most nanoparticles are thought to be sequestered by macrophages (Kupffer cells). To achieve effective cell-specific targeting of drugs and non-viral gene delivery vectors, improved mechanistic…
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Biophysicists unravel exact folding of a single gene
The way genes are folded determines their activity in our body. Leiden biophysicists are now the first to unravel this structure molecule-by-molecule. Publication on December 1st in Nature’s Scientific Reports.