As a virtually inexhaustible source, solar energy plays a major role in future global energy scenarios.

Promotores: Prof.dr. H. S. Overkleeft, Prof.dr. G. A. van der Marel

This thesis is a collection of experimental attempts to enhance photoluminescence of fluorescent molecules and quantum dots with single gold nanorods (GNRs) and relevant applications.

Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.

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Promotores: G.W. Canters, T.J. Aartsma

Promotor: Prof.dr. M.A.G.J. Orrit

PhD Defence

PURPOSE: To monitor the biodistribution of IgG1 aggregates upon subcutaneous (SC) and intravenous (IV) administration in mice and measure their propensity to stimulate an early immune response.

PhD Defence

A team of surgeons at Leiden University Medical Center and the Centre for Human Drug Research have removed a lung tumour that they pinpointed using fluorescence. This is the first time that this technique has been used in Europe in a patient with a lung tumour.

From molecule, to cell, to organism. The high-level drug research at the Cell Observatory aimes at reducing the spread of cancer and developing new medicines for tuberculosis.

How can novel bioimaging technologies and vertebrate model species be used to gain a better understanding of early cellular behaviours with the ultimate goal to increase our understanding of human development and disease processes?

PhD Defence

Promotor: T. Schmidt

The Microscopy Unit houses, maintains and coordinates most of the microscopy equipment of the IBL. The available equipment ranges from conventional light and fluorescence microscopes, to confocal laser scanning and electron microscopes. In addition, infrastructure is available for histology, including…

Can chemical safety assessment be improved with zebrafish embryo-based reporter assays?

Glycosidases are essential in fundamental biological processes and are responsible for the degradation of most (oligo)saccharides, glycolipids and glycoproteins.

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

Dutch researchers have developed a new tool to label DNA for studying chromosomes in live cells. The tool is non-invasive and can be applied in culture but also in living organisms, such as zebrafish embryos. The team published their findings in the journal Nucleic Acids Research.

To this day, all cyclophellitol-based inhibitors and ABPs have been close analogues of their natural substrate counterparts. As a result, these probes showed high selectivity towards their target glycosidases.

This is a yearly event where we move in one week from the physics of light through the composition of the microscope, fluorescence microscopy and end up with some image analysis All the lectures are backed by practicals to really put your obtained knowledge into practice

PhD Defence

PhD Defence

Control of Living Cells by Magnetic Fluorescent Nanoparticles

Deficiency of glucocerebrosidase (GBA) underlies Gaucher disease, a common lysosomal storage disorder. Carriership for Gaucher disease has recently been identified as major risk for parkinsonism. Presently, no method exists to visualize active GBA molecules in situ. We here report the design, synthesis…

Optical detection of a single electron using a single molecule has never been done. Leiden physicist Michel Orrit and his team have now identified a molecule that is sensitive enough to detect an electron at a distance of hundreds of nanometers. The results are published as a cover article in ChemPh…

PhD Defence

PhD Defence

This is a facility where research groups or small companies can apply for space if they need to culture human or animal cells.

Lecture

Single-molecule spectroscopy has become a powerful method for using organic fluorescent molecules in numerous applications.

Leiden physicists have managed to detect a single molecule called dibenzoterrylene in a new crystal, and found that it is a candidate component for a quantum network. Future quantum computers will need such a network to work together while maintaining their advantages. Publication in ChemPhysChem jo…

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Activity-based protein profiling (ABPP) is a functional proteomics technique for directly monitoring the expression of active enzymes in cell extracts and living cells. The technique relies on irreversible inhibitors equipped with reactive groups (warheads) that covalently attach to the active site…

Chapter 2 describes a post-synthetic methodology to introduce a fluorescent label in highly lipophilic, Pam3Cys based conjugates.

Dr. at the Department of organic chemistry, Wageningen University - the Nederlands

Painstaking research in Dutch and Portuguese archive materials, so far poorly assessed on the topic of social relations, reveals intense and intricate associations between different European individuals both in terms of ethnicity and social strata.

Spatially and Tempoprally Defined Microfluidics to Control Chemotactic Cells

How do macrophages and neutrophils contribute to metastatic onset?

Our facilities cover:

PhD Defence

How can you use robots and automatic recognition of microscopic images to test the effect of drugs exceptionally quickly?

Promotor: Prof.dr. T. Schmidt, Co-Promotor: Dr. ir. S.J.T. van Noort

Formulation of biopharmaceuticals

Can we build a biosensor to detect neuroblastoma markers in urine using the chemotaxis system in E. coli?

Establishing human induced pluripotent stem cell (iPSC) CRISPR-Cas9-based fluorescent reporter cell lines for NF-kB signalling. We will use this to enable studying the dynamic activation and downstream signalling of Toll-like receptor and cytokine signalling in iPSC-derived differentiated cells in a…

PhD Defence