197 search results for “fluorescent dyes” in the Public website
-
Accelerating the Photocatalytic Water Splitting in Catalyst−Dye Complexes
As a virtually inexhaustible source, solar energy plays a major role in future global energy scenarios.
-
A chemical biology approach for targeting of ligand-drug conjugates
Promotores: Prof.dr. H. S. Overkleeft, Prof.dr. G. A. van der Marel
-
enhancement of one-photon- and two-photon-excited single-molecule fluorescence by single gold nanorods
This thesis is a collection of experimental attempts to enhance photoluminescence of fluorescent molecules and quantum dots with single gold nanorods (GNRs) and relevant applications.
-
From non-labeled to fluorescent
This page is under construction.
-
Fluorescence Polarization Activity-Based Protein Profiling on Retaining Glycosidases
Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.
-
Fluorescence Correlation Spectroscopy on Electron Transfer Reaction: Probing Inter- and Intramolecular Redox Processes
Promotores: G.W. Canters, T.J. Aartsma
-
Stable single molecules for quantum optics and all-optical switches
Promotor: Prof.dr. M.A.G.J. Orrit
-
In vivo fluorescence imaging of IgG1 aggregates after subcutaneous and intravenous injection in mice
PURPOSE: To monitor the biodistribution of IgG1 aggregates upon subcutaneous (SC) and intravenous (IV) administration in mice and measure their propensity to stimulate an early immune response.
-
European first: Leiden specialists use fluorescence in removal of lung tumour
A team of surgeons at Leiden University Medical Center and the Centre for Human Drug Research have removed a lung tumour that they pinpointed using fluorescence. This is the first time that this technique has been used in Europe in a patient with a lung tumour.
-
Microscopy Unit
The Microscopy Unit houses, maintains and coordinates most of the microscopy equipment of the IBL. The available equipment ranges from conventional light and fluorescence microscopes, to confocal laser scanning and electron microscopes. In addition, infrastructure is available for histology, including…
-
Yang Shao
Science
-
ImageInLife: Training European experts in multilevel bioimaging, analysis and modelling of vertebrate development
How can novel bioimaging technologies and vertebrate model species be used to gain a better understanding of early cellular behaviours with the ultimate goal to increase our understanding of human development and disease processes?
-
Fluorescence-guided surgery: Clinical translation of targeted and non- targeted dyes
PhD Defence
-
Quantitative Super-Resolution Microscopy
Promotor: T. Schmidt
-
Joost Willemse
Science
-
High-throughput screening for developmental and reproductive toxicity
Can chemical safety assessment be improved with zebrafish embryo-based reporter assays?
-
Design and development of conformational inhibitors and activity-based probes for retaining glycosidases
Glycosidases are essential in fundamental biological processes and are responsible for the degradation of most (oligo)saccharides, glycolipids and glycoproteins.
-
Direct and two-step activity-based profiling of proteases and glycosidases
Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel
-
Cyclophellitol analogues for profiling of exo- and endo-glycosidases
To this day, all cyclophellitol-based inhibitors and ABPs have been close analogues of their natural substrate counterparts. As a result, these probes showed high selectivity towards their target glycosidases.
-
Intraoperative fluorescence imaging
PhD Defence
-
Accelerating the Photocatalytic Water Splitting in Catalyst−Dye Complexes
PhD Defence
-
Magnetic Microrheology
Control of Living Cells by Magnetic Fluorescent Nanoparticles
-
Ultrasensitive in situ visualization of active glucocerebrosidase molecules
Deficiency of glucocerebrosidase (GBA) underlies Gaucher disease, a common lysosomal storage disorder. Carriership for Gaucher disease has recently been identified as major risk for parkinsonism. Presently, no method exists to visualize active GBA molecules in situ. We here report the design, synthesis…
-
Fluorescence-enhanced surgical navigation
PhD Defence
-
Single molecules show promise to optically detect single electrons
Optical detection of a single electron using a single molecule has never been done. Leiden physicist Michel Orrit and his team have now identified a molecule that is sensitive enough to detect an electron at a distance of hundreds of nanometers. The results are published as a cover article in ChemPh…
-
Fluorescent Chemical Probes for Clinical Imaging Applications
Lecture
-
Towards Optical Detection of a Single Electron
Single-molecule spectroscopy has become a powerful method for using organic fluorescent molecules in numerous applications.
-
Single Molecule Detected for Use in Quantum Network
Leiden physicists have managed to detect a single molecule called dibenzoterrylene in a new crystal, and found that it is a candidate component for a quantum network. Future quantum computers will need such a network to work together while maintaining their advantages. Publication in ChemPhysChem jo…
-
Relative quantification of proteasome activity by activity-based protein profiling and LC-MS/MS
Activity-based protein profiling (ABPP) is a functional proteomics technique for directly monitoring the expression of active enzymes in cell extracts and living cells. The technique relies on irreversible inhibitors equipped with reactive groups (warheads) that covalently attach to the active site…
-
Self adjuvanting immunopeptides: Design and synthesis
Chapter 2 describes a post-synthetic methodology to introduce a fluorescent label in highly lipophilic, Pam3Cys based conjugates.
-
Controlled Chemotaxis
Spatially and Tempoprally Defined Microfluidics to Control Chemotactic Cells
-
Role of leukocytes in metastasis formation in a zebrafish
How do macrophages and neutrophils contribute to metastatic onset?
-
Sociabilidade do Brasil Neerlandês (1630 - 1654)
Painstaking research in Dutch and Portuguese archive materials, so far poorly assessed on the topic of social relations, reveals intense and intricate associations between different European individuals both in terms of ethnicity and social strata.
-
Teris van Beek
Dr. at the Department of organic chemistry, Wageningen University - the Nederlands
-
Facilities
Our facilities cover:
-
Fluorescence Polarization Activity-Based Protein Profiling on Retaining Glycosidases
PhD Defence
-
Fluorescent gene reporters in human pluripotent stem cells
PhD Defence
-
spFRET studies of nucleosome dynamics modulated by histone modifications, histone variants and neighboring nucleosomes
Promotor: Prof.dr. T. Schmidt, Co-Promotor: Dr. ir. S.J.T. van Noort
-
Vertebrate automated screening technology (VAST)
How can you use robots and automatic recognition of microscopic images to test the effect of drugs exceptionally quickly?
-
RINSE- development of a RapId Neuroblastoma Sensor that utilizes native microbe interactions
Can we build a biosensor to detect neuroblastoma markers in urine using the chemotaxis system in E. coli?
-
Formulation of biopharmaceuticals
Formulation of biopharmaceuticals
-
Systems microscopy-based drug target discovery in pathogen-meidated inflammatory respons signalling
Establishing human induced pluripotent stem cell (iPSC) CRISPR-Cas9-based fluorescent reporter cell lines for NF-kB signalling. We will use this to enable studying the dynamic activation and downstream signalling of Toll-like receptor and cytokine signalling in iPSC-derived differentiated cells in a…
-
Arabic in context: celebrating 400 years of Arabic at Leiden university
The edited volume 'Arabic in context: celebrating 400 years of Arabic at Leiden University' edited by Ahmad Al-Jallad is out. The volume contains the contributions to the 2013 conference with the same title, held at Leiden University and was published as part of Brill's the Studies in Semitics Language…
-
Lipid bilayers decorated with photosensitive ruthenium complexes
Promotor: E. Bouwman, Co-promotor: S. Bonnet
-
ZF-CANCER - Developing high-throughput bioassays for human cancers in zebrafish
How can zebrafish research help to understand and fight human cancer?
-
Mathematical modeling of cellular stress pathways for mechanistic understanding of chemical-induced liver injury
Cellular stress response pathways are activated upon exposure to chemicals and help organisms deal with various molecular dangers (e.g. oxidative stress). When these pathways become hyper-activated an adverse response takes place and cells die, which may lead to organism-level toxicity such as drug-induced…
-
Our approach
Our group operates at the cross-roads of different disciplines: molecular and cellular microbiology, biochemistry, structural biology and biophysics. The group harbors expertise in a multitude of molecular and cellular approaches including:
-
Expertise
The CIGR brings together a diverse range of unique expertise in genome research rooted in biology, chemistry and physics. Members of the CIGR investigate genome folding and genome transactions. An important aspect is direct as well as long term relevance for medicine. The available expertise extends…
-
Identification of novel targets in prostate cancer progression
Promotor: Prof.dr. B. van de Water, Co-promotor: E.H.J. Danen
-
Cationic liposomes loaded with a synthetic long Peptide and poly(i:C): a defined adjuvanted vaccine for induction of antigen-specific T cell
For effective cancer immunotherapy by vaccination, co-delivery of tumour antigens and adjuvants to dendritic cells and subsequent activation of antigen-specific cytotoxic T cells (CTLs) is crucial. In this study, a synthetic long peptide (SLP) harbouring the model CTL epitope SIINFEKL was encapsulated…