The Microscopy Unit houses, maintains and coordinates most of the microscopy equipment of the IBL. The available equipment ranges from conventional light and fluorescence microscopes, to confocal laser scanning and electron microscopes. In addition, infrastructure is available for histology, including…

This is a yearly event where we move in one week from the physics of light through the composition of the microscope, fluorescence microscopy and end up with some image analysis All the lectures are backed by practicals to really put your obtained knowledge into practice

Promotor: T. Schmidt

This thesis is a collection of experimental attempts to enhance photoluminescence of fluorescent molecules and quantum dots with single gold nanorods (GNRs) and relevant applications.

Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.

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Electron microscopy has become an extremely important techniquein a wide variety of elds.

The aim of this facility is to accelerate biomedical research by providing access to advanced light and electron microscopy and by sharing expertise about sample preparation, imaging modalities, data storage and image analysis.

Science

This thesis described the development of novel scanning tunneling microscopy techniques to investigate strongly correlated electronic states in quantum matter.

Establishing human induced pluripotent stem cell (iPSC) CRISPR-Cas9-based fluorescent reporter cell lines for NF-kB signalling. We will use this to enable studying the dynamic activation and downstream signalling of Toll-like receptor and cytokine signalling in iPSC-derived differentiated cells in a…

Promotor: Prof.dr. J.M. van Ruitenbeek, Prof.dr. R.M. Tromp

In this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms.

In this thesis, we describe the latest advances in SQUID-detected Magnetic Resonance Force Microscopy (MRFM).

In this thesis, we show how MRFM can usefully contribute to the field of condensed-matter.

Intrigued by the way cells autonomously regulate their fate, we strive to understand and visualize cellular processes as the basis of Cell Signaling.

The dissertation describes TEM experiments on heterogeneous catalysts.

Optical detection of a single electron using a single molecule has never been done. Leiden physicist Michel Orrit and his team have now identified a molecule that is sensitive enough to detect an electron at a distance of hundreds of nanometers. The results are published as a cover article in ChemPh…

Our facilities cover:

Promotores: Prof.dr. H.P. Spaink & Prof.dr. P.C.W. Hogendoorn, Co-promotor: Dr. M.J.M. Schaaf

Promotores: Prof.dr. C.U. Keller, Prof.dr. H.C. Gerritsen

Promotor: T.H. Oosterkamp

Promotor: Prof.dr. M.A.G.J. Orrit

How can you use robots and automatic recognition of microscopic images to test the effect of drugs exceptionally quickly?

We explore the possibilities to combine magnetic resonance techniques with atomic force microscopy together in a single microscope: the MRI-AFM, also called Magnetic Resonance Force Microscopy (MRFM).

Promotores: G.W. Canters, T.J. Aartsma

Leiden physicists have managed to detect a single molecule called dibenzoterrylene in a new crystal, and found that it is a candidate component for a quantum network. Future quantum computers will need such a network to work together while maintaining their advantages. Publication in ChemPhysChem jo…

Deficiency of glucocerebrosidase (GBA) underlies Gaucher disease, a common lysosomal storage disorder. Carriership for Gaucher disease has recently been identified as major risk for parkinsonism. Presently, no method exists to visualize active GBA molecules in situ. We here report the design, synthesis…

PURPOSE: To monitor the biodistribution of IgG1 aggregates upon subcutaneous (SC) and intravenous (IV) administration in mice and measure their propensity to stimulate an early immune response.

High throughput microscopy

The purpose of this study was to investigate the formation and growth kinetics of complexes between proteins and oppositely charged polyelectrolytes.

The CIGR brings together a diverse range of unique expertise in genome research rooted in biology, chemistry and physics. Members of the CIGR investigate genome folding and genome transactions. An important aspect is direct as well as long term relevance for medicine. The available expertise extends…

Nucleoid associated proteins maintain the architecture of the bacterial chromosome and regulate gene expression, hinting that their role as transcription factors may involve local three-dimensional chromosome re-modelling.

Promotor: B. van de Water

Promotor: B. van de Water

From molecule, to cell, to organism. The high-level drug research at the Cell Observatory aimes at reducing the spread of cancer and developing new medicines for tuberculosis.

The Netherlands Organisation for Scientific Research (NWO) has made a subsidy of 17 million euros available to further develop a Netherlands network for electron microscopy (NEMI). The network comprises five UMCs and eight universities, with Utrecht in the coordinating role. From Leiden, the Institute…

A team of surgeons at Leiden University Medical Center and the Centre for Human Drug Research have removed a lung tumour that they pinpointed using fluorescence. This is the first time that this technique has been used in Europe in a patient with a lung tumour.

How can novel bioimaging technologies and vertebrate model species be used to gain a better understanding of early cellular behaviours with the ultimate goal to increase our understanding of human development and disease processes?

What is the mechanism of action preceding a physiological effects induced by metallic nanoparticles?

Our group operates at the cross-roads of different disciplines: molecular and cellular microbiology, biochemistry, structural biology and biophysics. The group harbors expertise in a multitude of molecular and cellular approaches including:

In this thesis the combinatorial use of bioorthogonal labelling and Electron Microscopy (EM)-based imaging techniques is explored to enable observations of specific molecular targets in their ultrastructural context within the cell.

The CIGR comprises research groups from the Institute of Biology Leiden (IBL), the Leiden Institute of Chemistry (LIC), the Leiden Academic Centre for Drug Research (LACDR) and the Leiden Institue of Physics (LION).

This specialisation focuses on two important trends in health care: early diagnosis and developing the most targeted patient-specific treatment possible with the shortest possible recovery time and minimal side effects.

From surface science towards industrial practice using high-pressure scanning tunneling microscopy.

Chinese and Leiden scientists came together in Leiden to study the intricacies of modern visual techniques.

Formulation of biopharmaceuticals

Advanced fluorescence microscopy techniques have revealed how steroid receptors move inside the nucleus. The results were published by a team from Leiden University and the Erasmus Medical Center in Rotterdam, led by IBL-researcher Marcel Schaaf.