462 search results for “fluorescence microscopy” in the Public website
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Microscopy Unit
The Microscopy Unit houses, maintains and coordinates most of the microscopy equipment of the IBL. The available equipment ranges from conventional light and fluorescence microscopes, to confocal laser scanning and electron microscopes. In addition, infrastructure is available for histology, including…
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Quantitative Super-Resolution Microscopy
Promotor: T. Schmidt
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Joost Willemse
Science
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enhancement of one-photon- and two-photon-excited single-molecule fluorescence by single gold nanorods
This thesis is a collection of experimental attempts to enhance photoluminescence of fluorescent molecules and quantum dots with single gold nanorods (GNRs) and relevant applications.
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From non-labeled to fluorescent
This page is under construction.
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Fluorescence Polarization Activity-Based Protein Profiling on Retaining Glycosidases
Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.
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eV-TEM: Transmission Electron Microscopy with few-eV Electrons
Electron microscopy has become an extremely important techniquein a wide variety of elds.
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Light and Electron Microscopy Facility
The aim of this facility is to accelerate biomedical research by providing access to advanced light and electron microscopy and by sharing expertise about sample preparation, imaging modalities, data storage and image analysis.
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Probing quantum materials with novel scanning tunneling microscopy techniques
This thesis described the development of novel scanning tunneling microscopy techniques to investigate strongly correlated electronic states in quantum matter.
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Cryo-electron microscopy scientist
Science
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Systems microscopy-based drug target discovery in pathogen-meidated inflammatory respons signalling
Establishing human induced pluripotent stem cell (iPSC) CRISPR-Cas9-based fluorescent reporter cell lines for NF-kB signalling. We will use this to enable studying the dynamic activation and downstream signalling of Toll-like receptor and cytokine signalling in iPSC-derived differentiated cells in a…
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Low-Energy Electron Microscopy on Two-Dimensional Systems: Growth, Potentiometry and Band Structure Mapping
Promotor: Prof.dr. J.M. van Ruitenbeek, Prof.dr. R.M. Tromp
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Two-photon multifocal microscopy for in vivo single-molecule and single-particle imaging
In this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms.
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Advances in SQUID-detected Magnetic Resonance Force Microscopy
In this thesis, we describe the latest advances in SQUID-detected Magnetic Resonance Force Microscopy (MRFM).
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Magnetic resonance force microscopy for condensed matter
In this thesis, we show how MRFM can usefully contribute to the field of condensed-matter.
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Single molecules show promise to optically detect single electrons
Optical detection of a single electron using a single molecule has never been done. Leiden physicist Michel Orrit and his team have now identified a molecule that is sensitive enough to detect an electron at a distance of hundreds of nanometers. The results are published as a cover article in ChemPh…
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Transmission electron microscopy on live catalysts
The dissertation describes TEM experiments on heterogeneous catalysts.
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Facilities
Our facilities cover:
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mycobacterial infection: analysis by a combination of light and electron microscopy
Promotores: Prof.dr. H.P. Spaink & Prof.dr. P.C.W. Hogendoorn, Co-promotor: Dr. M.J.M. Schaaf
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Lasers, lenses and light curves: adaptive optics microscopy and peculiar transiting exoplanets
Promotores: Prof.dr. C.U. Keller, Prof.dr. H.C. Gerritsen
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Nuclear magnetic resonance force microscopy at millikelvin temperatures
Promotor: T.H. Oosterkamp
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Stable single molecules for quantum optics and all-optical switches
Promotor: Prof.dr. M.A.G.J. Orrit
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Fluorescence Correlation Spectroscopy on Electron Transfer Reaction: Probing Inter- and Intramolecular Redox Processes
Promotores: G.W. Canters, T.J. Aartsma
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Single Molecule Detected for Use in Quantum Network
Leiden physicists have managed to detect a single molecule called dibenzoterrylene in a new crystal, and found that it is a candidate component for a quantum network. Future quantum computers will need such a network to work together while maintaining their advantages. Publication in ChemPhysChem jo…
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In vivo fluorescence imaging of IgG1 aggregates after subcutaneous and intravenous injection in mice
PURPOSE: To monitor the biodistribution of IgG1 aggregates upon subcutaneous (SC) and intravenous (IV) administration in mice and measure their propensity to stimulate an early immune response.
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Ultrasensitive in situ visualization of active glucocerebrosidase molecules
Deficiency of glucocerebrosidase (GBA) underlies Gaucher disease, a common lysosomal storage disorder. Carriership for Gaucher disease has recently been identified as major risk for parkinsonism. Presently, no method exists to visualize active GBA molecules in situ. We here report the design, synthesis…
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High throughput microscopy for cellular adaptive stress response pathways in drug adversity
High throughput microscopy
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formation and growth by nanoparticle tracking analysis and flow imaging microscopy
The purpose of this study was to investigate the formation and growth kinetics of complexes between proteins and oppositely charged polyelectrolytes.
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European first: Leiden specialists use fluorescence in removal of lung tumour
A team of surgeons at Leiden University Medical Center and the Centre for Human Drug Research have removed a lung tumour that they pinpointed using fluorescence. This is the first time that this technique has been used in Europe in a patient with a lung tumour.
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High throughput microscopy of mechanism-based reporters in druginduced liver injury
Promotor: B. van de Water
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Systems microscopy to unravel cellular stress response signalling in drug induced liver injury
Promotor: B. van de Water
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17 mln subsidy to develop electron microscopy in the Netherlands
The Netherlands Organisation for Scientific Research (NWO) has made a subsidy of 17 million euros available to further develop a Netherlands network for electron microscopy (NEMI). The network comprises five UMCs and eight universities, with Utrecht in the coordinating role. From Leiden, the Institute…
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ImageInLife: Training European experts in multilevel bioimaging, analysis and modelling of vertebrate development
How can novel bioimaging technologies and vertebrate model species be used to gain a better understanding of early cellular behaviours with the ultimate goal to increase our understanding of human development and disease processes?
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Development of an Adverse Outcome Pathway (AOP) for metallic nanoparticles
What is the mechanism of action preceding a physiological effects induced by metallic nanoparticles?
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A bioorthogonal chemistry approach to the study of biomolecules in their ultrastructural cellular context
In this thesis the combinatorial use of bioorthogonal labelling and Electron Microscopy (EM)-based imaging techniques is explored to enable observations of specific molecular targets in their ultrastructural context within the cell.
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Our approach
Our group operates at the cross-roads of different disciplines: molecular and cellular microbiology, biochemistry, structural biology and biophysics. The group harbors expertise in a multitude of molecular and cellular approaches including:
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Imaging complex model catalysts in action
From surface science towards industrial practice using high-pressure scanning tunneling microscopy.
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Redmar Vlieg
Science
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Imaging & Intervention (MSc)
This specialisation focuses on two important trends in health care: early diagnosis and developing the most targeted patient-specific treatment possible with the shortest possible recovery time and minimal side effects.
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Learning together about electron microscopy
Chinese and Leiden scientists came together in Leiden to study the intricacies of modern visual techniques.
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Formulation of biopharmaceuticals
Formulation of biopharmaceuticals
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Movements of steroid receptors inside the cell nucleus unraveled
Advanced fluorescence microscopy techniques have revealed how steroid receptors move inside the nucleus. The results were published by a team from Leiden University and the Erasmus Medical Center in Rotterdam, led by IBL-researcher Marcel Schaaf.
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Novel detectors and algorithms for electron nano-crystallography
Promotor: Prof.dr. J.P. Abrahams, Prof.dr. M. van Heel
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Irene Groot
Science
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Digital dissection and remote microscopy lessons
Due to the corona crisis, they had to switch to online education halfway during their course: associate professor Marcel Schaaf and PhD candidate Michiel Hooykaas of the Institute of Biology Leiden talk about digital practicals, online lectures and their biggest obstacle: exams.
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Two-photon microscope captures plant cells
Leiden physicists are helping Wageningen plant researchers to study unpredictable plant embryos. For this, they are using a novel two-photon fluorescence microscope, aided by a 30 thousand euro ZonMW grant.
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Michel Orrit wins Physica Prize 2016
Michel Orrit was awarded the Physica prize 2016 for his groundbreaking work on single molecule spectroscopy.
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NWO grant for super fast analysis of disease progression
New funding allows IBL-researcher Herman Spaink to purchase a measurement device for more studies on human diseases such as cancer and tuberculosis using zebrafish as the model system.
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Visualizing strongly-correlated electrons with a novel scanning tunneling microscope
Materials with strongly correlated electrons show some of the most mysterious and exotic phases of quantum matter, such as unconventional superconductivity, quantum criticality and strange metal phase.
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Core Facilities
Core Facilities are shared resources that provide access to instruments, technologies and services to researchers across the Institute of Biology. They are staffed by scientists with vast experience in their field, ensuring that all researchers can count not only on the latest available technology…